Qiagen QIAcuity 911000 User Manual page 159

Concentration (copies/ul) – This column shows the concentration assigned to each target or

channel.
CI (95%) – This column shows the value of the confidence interval at a 95% confidence level.

Edited fraction – This column shows the edited fraction for the edited target as percentage.

CI (95%) – This column shows the value of the confidence interval for the edited target at a

95% confidence level.
Replicates are treated different for multiplex and simplex test setup:
Multiplex test (configured wildtype and edited targets are part of the same reactionmix):

Replicates are analyzed as separate wells but in addition the mean concentration and mean
edited fraction values together with the corresponding mean CI are provided on demand.
On the right side above the table there is checkbox which allows the user to show mean
values for replicates in addition. By default, the button is unchecked, and results are displayed
without mean values. When the button is checked the list view still shows independent rows
for each selected well, but replicates are grouped together. Replicates from the same group
are next to each other in the list view. The list view is extended by 4 columns indicating
following mean values:
Mean concentration value

CI (95%) - CI of mean concentration as percentage

Mean edited fraction as percentage

CI (95%) - CI of mean edited fraction as percentage

Simplex test (configured wildtype and edited targets are part of different reaction mixes):

For tests having the configured targets in different wells/reaction mixes, the mean result is
calculated and shown for replicates in the selection. The list view is extended by 4 columns
indicating following mean values:
Mean concentration value

CI (95%) - CI of mean concentration as percentage

Mean edited fraction as percentage

CI (95%) - CI of mean edited fraction as percentage

QIAcuity User Manual 07/2020 159