Qiagen QIAcuity 911000 User Manual page 168

In case that targets are in different reaction mixes each combination of target of interest and
reference targets in one sample throughout the selected wells is shown in the diagram with a point
for the result. To view detailed information, hold the mouse pointer over its corresponding point. A
tooltip with detailed information opens.
To view the mean values for replicates, click Show mean values for replicates. If the user clicks into
the checkbox to select the mean representation for replicates, the points of individual replicates
disappear and only one point is shown at the sample label that represents the mean copies per
genome value of the replicates. When there are no replicates within selected wells the points don't
change. The corresponding well IDs of the replicates are shown on the x axis. This is also applied
in same way by default to the view where targets are in different reaction mixes. In this case
individual replicate results are not available. Mean values are calculated and shown over all
replicates that are included in the well selection only. If there are further replicates of the same
sample which are not included in well selection, they are not considered in the calculated mean
value and show their individual results values.
Note: If the selected analysis source contains replicates of the reference sample, your results can be
calculated only by using mean results for replicates. In this case the "Show mean values for
replicates" check box is marked and can't be changed. In this case there is a warning message
above the diagram title and when the user hover on the checkbox, a tooltip appears to inform the
user that selected analysis source contains replicates of the reference sample and result is calculated
only by using mean results for replicates.
To add the point diagram to the report, click Add to report. For more information on reports, see
section Reports.
QIAcuity User Manual 07/2020 168