Qiagen QIAcuity 911000 User Manual page 156

mean mutant fraction value of the replicates. When there are no replicates within selected wells for
mutant target the points don't change. The corresponding well IDs of the replicates are shown on
the x axis. This is also applied in same way by default to the view where targets are in different
reaction mixes. In this case individual replicate results are not available. Mean values are calculated
and shown over all replicates that are included in the well selection only. If there are further
replicates of the same sample which are not included in well selection, they are not considered in
the calculated mean value and show their individual results values.
To add the point diagram to the report, click Add to report. For more information about reports,
see section Reports.
Concentration diagram for mutation detection
The Concentration diagram tab shows a diagram that displays the distribution of concentration
values in the wells together with their confidence intervals. A concentration diagram has two axes.
The x-axis shows the analyzed wells and samples, and the y-axis represents the concentration
values. The y-axis scale can be modified using the buttons located on the left below each graph.
The buttons are visible when you hold the pointer over a graph. To view the values on a linear
scale, click lin. To view the values on a logarithmic scale, click log10.
To view an additional toolbar that enables actions related to the diagram, such as downloading
the plot, hold the pointer over the diagram. For more details about the toolbar, refer to section
Diagram options.
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