Qiagen QIAcuity 911000 User Manual page 147

1D Scatterplot for the reference channel.
Lasso mode for partition assignment
Alternative of changing threshold values the user can assign partitions using a free hand selection
in the 2D Scatterplot view (Lasso mode). When the user marked points by lasso for two channels
or targets all thresholds on 1D Scatterplot for them disappear and can't be modified before
switching back to threshold mode using the auto threshold button. When the user clicks recalculate
button, changes in threshold selection affects 2D Scatterplot results as well.
Channels which are not used in 2D Scatterplot with lasso mode do still show the threshold mode
in the 1D Scatterplot view.
Refer to 2D Scatterplot tab for absolute quantification for more details regarding the assignment of
partitions using the lasso mode.
2D Scatterplot tab for absolute quantification
The 2D Scatterplot tab shows a 2D Scatterplot view that displays the fluorescence intensity from two
selected channels or targets, allowing for a comparison and evaluation of the fluorescence from
two sources.
To create a 2D Scatterplot, assign targets or channels to the x-axis and y-axis using the drop down
menus When 2 of the selected sources for analysis are assigned, the 2D Scatterplot view is
displayed. The axes represent the value of fluorescence intensity. The ranges of the axis are aligned
QIAcuity User Manual 07/2020 147