Qiagen QIAcuity 911000 User Manual page 152

Setting up a mutation detection analysis
1. Click the relevant wells in the Select wells pane. For more information, see Selecting wells for
analysis.
2. Select the applicable wild type target from the Wild type target list.
3. Select the applicable mutant target from the Mutant target list.
4. To view the results of the analysis, click Show results.
The results are divided into several tabs. To view the contents of the tab, click the tab title.
List tab for mutation detection
The List tab contains a table with an overview of the analyzed wells. The following columns are
available in the table. The following columns are available in the table:
Well identifier which represents the well position on the plate layout

Sample/NTC/Control – This column shows the sample, NTC, or control name with its

corresponding icon that identifies the sample or indicates whether the entry is an NTC or
control.
Reaction mix – This column contains the icon and the name of the Reaction mix..

Target – This column shows all target names with its corresponding target type. Targets which

were selected as wild type or mutant are marked
Concentration (copies/ul) – This column shows the concentration assigned to each target.
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QIAcuity User Manual 07/2020
accordingly.
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