The partitions heatmap of a selected target.
To add any of the heatmaps to the report, click Add to report next to the relevant diagram. For
more information on reports, see section .
Histogram tab for absolute quantification
The Histogram tab displays graphs that visualize the fluorescence values of selected wells for the
selected target or channel. One histogram view is created for each selected target or channel. The
histogram views are sorted by channel position in imaging ("Green", "Yellow", "Orange", "Red",
"Crimson"), separated by a horizontal line.
Each histogram has two axes. The x-axis represents the relative fluorescence intensity. The y-axis
represents the number of partitions having that fluorescence intensity. The values on the y-axis have
two available scales – linear and logarithmic.
To view an additional toolbar that enables actions related to the diagram, such as downloading
the plot, hold the pointer over the diagram. For more details about the toolbar, refer to section
Diagram options.
QIAcuity User Manual 07/2020
140