Qiagen QIAcuity 911000 User Manual page 165

CI (95%) – This column shows the value of the confidence interval for the target of interest at a

95% confidence level.
Replicates are treated different for multiplex and simplex test setup:
Multiplex test (configured target of interest and reference targets are part of the same reaction

mix):
On the right side above the table there is checkbox which allows the user to show mean
values for replicates in addition. By default, the button is unchecked, and results are displayed
without mean values. When the button is checked the list view still shows independent rows
for each selected well, but replicates are grouped together. Replicates from the same group
are next to each other in the list view. The list view is extended by 4 columns indicating
following mean values:
Mean concentration value

CI (95%) - CI of mean concentration as percentage

Mean copies/genome

CI (95%) - CI of mean mutation fraction as percentage

Simplex test (configured target of interest and reference targets are part of different reaction

mixes):
For tests having the configured targets in different wells/reaction mixes, the mean result is
calculated and shown for replicates in the selection. The list view is extended by 4 columns
indicating following mean values:
Mean concentration value

CI (95%) - CI of mean concentration as percentage

Mean copies/genome

CI (95%) - CI of mean mutation fraction as percentage

QIAcuity User Manual 07/2020 165