Qiagen QIAcuity 911000 User Manual page 143

Lasso mode for partition assignment
Alternative of changing threshold values the user can assign partitions using a free hand selection
in the 2D Scatterplot view (Lasso mode). When the user marked points by lasso for two channels
or targets all thresholds on histogram for them disappear and can't be modified before switching
back to threshold mode using the auto threshold button. When the user clicks recalculate
button, changes in threshold selection affects 2D Scatterplot results as well.
Channels which are not used in 2D Scatterplot with lasso mode do still show the threshold mode in
the histogram view.
Selected channel with active lasso mode
Refer to 2D Scatterplot tab for absolute quantification for more details regarding the assignment of
partitions using the lasso mode.
1D Scatterplot tab for absolute quantification
The 1D Scatterplot tab shows one 1D Scatterplot view for each analyzed target or channel. If
there are more than one scatterplot views, they are separated with a horizontal line. The 1D
Scatterplot views are sorted by channel position in imaging ("Green", "Yellow", "Orange",
"Red", "Crimson").
The title of a 1D Scatterplot view shows the related channel name including the dot channel color
indicator, and if defined also the target name. If more than one well is selected also the number of
wells is shown.
QIAcuity User Manual 07/2020 143