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Summary of Contents for Qiagen TissueLyser II
- Page 1 May 2016 TissueLyser II User Manual For disruption of up to 48 or 192 biological samples Sample to Insight__...
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Page 2: Table Of Contents
Translations of warnings and cautions ................ 9 Introduction ........................15 About this user manual ...................15 Intended use of the TissueLyser II ................15 Damage to the clamps of the TissueLyser II ............16 Requirements for TissueLyser II users ..............16 General Description ......................17 TissueLyser II principle .....................17 Features of the TissueLyser II system ................18... - Page 3 Maintenance Procedures ....................35 Servicing ......................35 Regular maintenance ....................35 Cleaning agents ....................36 Regular safety checks .....................37 Troubleshooting Guide ......................39 Error codes displayed by the TissueLyser II ..............39 Appendix A ........................40 Technical data .......................40 Environmental conditions ..................40 Operating conditions ..................40 Transportation conditions ...................40 Storage conditions ....................40...
- Page 4 Appendix B ........................44 Liability clause .......................44 Notes ......................45 Notes ......................46 TissueLyser II User Manual 05/2016...
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Page 5: Safety Information
The TissueLyser II should only be used for the applications described in the TissueLyser Handbook. The TissueLyser II should only be used in combination with accessories approved by QIAGEN (see “Features of the TissueLyser II system,” page 18). Do not continue to use the TissueLyser II or TissueLyser accessory if it is damaged. -
Page 6: Electrical Safety
Avoid spilling water or chemicals onto the TissueLyser II. Damage caused by water or chemical spillage will void your warranty. In case of emergency, switch off the TissueLyser II at the power switch and unplug the power cord from the power outlet. -
Page 7: Environment
It has been subjected to severe transport stresses. If the TissueLyser II is not in use, switch it off at the power switch. If the TissueLyser II will not be used for a prolonged period of time, unplug the power cord from the power outlet. -
Page 8: Chemicals
The responsible body (e.g., laboratory manager) must take the necessary precautions to ensure that the surrounding workplace is safe, and that TissueLyser II operators are suitably trained and not exposed to hazardous levels of infectious agents as defined in the applicable Safety Data Sheets (SDSs) or OSHA,* ACGIH, †... -
Page 9: Waste Disposal
Used labware, such as sample tubes, may contain hazardous chemicals or infectious agents from the sample disruption process. Such wastes must be collected and disposed of properly according to local safety regulations. For information on how to dispose of the TissueLyser II, see Appendix A (page 41). - Page 10 Risk of personal injury and material damage (W1) Improper use of the TissueLyser II may cause personal injuries or damage to the instrument. Do not attempt to move the TissueLyser II during operation. The TissueLyser II should only be operated by qualified personnel who have been appropriately trained.
- Page 11 Intentional interruption is prohibited. Lethal voltages inside the TissueLyser II When the TissueLyser II is connected to line power, terminals may be live, and opening the TissueLyser II or removing parts is likely to expose live parts. Gefahr durch Stromschlag Jede Unterbrechung des Schutzleiters (Erdungs- bzw.
- Page 12 Risque d’explosion Le TissueLyser II est conçu pour être utilisé avec les réactifs et substances fournis avec les kits QIAGEN. L’utilisation d’autres réactifs et substances présente des risques d’incendie ou d’explosion. WARNING Samples containing infectious agents (W5) Some samples used with the TissueLyser II may contain infectious agents. Handle such samples with the greatest of care and in accordance with the required safety regulations.
- Page 13 WARNING Hazardous chemicals (W6) Some chemicals used with the TissueLyser II may be hazardous or may become hazardous after completion of the sample disruption process. Always wear safety glasses, gloves, and a lab coat. The responsible body (e.g., laboratory manager) must take the necessary precautions to...
- Page 14 Grinding Jar Set peut détériorer le matériel ou provoquer des accidents corporels. S’assurer que les deux adaptateurs ou les deux cylindres de broyage ont un poids similaire. Ne pas faire fonctionner le TissueLyser II avec un seul adaptateur ou un seul cylindre de broyage.
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Page 15: Introduction
Introduction Thank you for choosing the TissueLyser II system. We are confident it will become an integral part of your laboratory. Before using the TissueLyser II, it is essential you read this user manual carefully and pay particular attention to the safety information. The instructions and safety information in the user manual must be followed to ensure safe operation of the TissueLyser II and to maintain the TissueLyser II in a safe condition. -
Page 16: Damage To The Clamps Of The Tissuelyser Ii
(service technicians) *Use of the TissueLyser II with non-QIAGEN adapters has been tested by other suppliers (e.g., Roche recommend use of the TissueLyser II with GS FLX Titanium emPCR Shaker Adapters, LV, which are accessories for the Genome Sequencer FLX Instrument). -
Page 17: General Description
General Description The TissueLyser II is a flexible bead mill designed for rapid and efficient disruption of up to 48 or 192 samples at the same time. A wide variety of biological samples can be processed, including human, animal, and plant tissues as well as yeast and bacteria. Each sample is simultaneously disrupted and homogenized by high-speed shaking with a bead in a sealed tube (bead milling). -
Page 18: Features Of The Tissuelyser Ii System
9 disruption parameters can be stored. Figure 1. TissueLyser II (closed). The transparent hood is lowered, and the clamps at the front of the instrument are empty. Note: The maximum oscillation frequency may drop down to 25 Hz, depending on local fluctuations in the mains voltage. - Page 19 Figure 2. TissueLyser II (open). The transparent hood is raised, and a TissueLyser Adapter Set is installed. TissueLyser II User Manual 05/2016...
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Page 20: Tissuelyser Adapter Set 2 X 24
“Time min/sec” display, with “–” and “+” keys for adjusting disruption time. “START” and “STOP” keys for starting and stopping operation of the TissueLyser II. TissueLyser Adapter Set 2 x 24 The TissueLyser Adapter Set 2 x 24 (cat. no. 69982) allows disruption of 48 samples in parallel using standard 2 ml microcentrifuge tubes (e.g., Eppendorf Safe-Lock micro test tubes... -
Page 21: Tissuelyser Adapter Set 2 X 96
96% ethanol. For more information, see the product sheet supplied with the adapter set. Figure 5. TissueLyser Adapter Set 2 x 96. Each adapter consists of a top plate and a bottom plate for holding a rack of capped collection microtubes. TissueLyser II User Manual 05/2016... -
Page 22: Grinding Jar Set, S. Steel
The Grinding Jar Set, Teflon (cat. no. 69986) allows disruption of 2 large samples in parallel using Teflon grinding balls (Figure 7). Sample disruption can be carried out at room temperature. For more information, see the product sheet supplied with the Grinding Jar Set. TissueLyser II User Manual 05/2016... -
Page 23: Tissuelyser Single-Bead Dispensers
The dispensers can be cleaned with water or detergent. For more information, see the product sheet supplied with the TissueLyser Single-Bead Dispenser. Figure 8. TissueLyser Single-Bead Dispenser. The dispenser dispenses individual beads into any sample container. TissueLyser II User Manual 05/2016... -
Page 24: Tissuelyser Bead Dispensers, 96-Well
The dispensers can be cleaned with water or detergent. For more information, see the product sheet supplied with the TissueLyser Bead Dispenser, 96-Well. Figure 9. TissueLyser Bead Dispenser, 96-Well. The dispenser dispenses 96 beads into a rack of collection microtubes. TissueLyser II User Manual 05/2016... -
Page 25: Beads
Beads The following beads for the TissueLyser II are available from QIAGEN: Stainless Steel Beads, 5 mm (cat. no. 69989) Stainless Steel Beads, 7 mm (cat. no. 69990) Tungsten Carbide Beads, 3 mm (cat. no. 69997) Applications The TissueLyser II enables disruption and homogenization of: ... -
Page 26: Installation Procedures
Make a note of how the TissueLyser II is packed. If returning the instrument to QIAGEN for repair, it needs to be repacked using the original packaging. When lifting the TissueLyser II, place both hands below the base of the instrument. -
Page 27: Site Requirements
Site requirements Place the TissueLyser II on a flat, stable surface, and ensure that there is sufficient space around and under the instrument for ventilation. The TissueLyser II is for indoor use only under the following conditions: ... -
Page 28: Power Requirements
The power line to the TissueLyser II should be voltage-regulated and surge-protected. Make sure that the voltage rating of the TissueLyser II (see the plate on the back of the instrument) is compatible with the AC voltage available at the installation site. -
Page 29: Installation Of A Tissuelyser Adapter Set Or Grinding Jar Set
Installation of a TissueLyser Adapter Set or Grinding Jar Set The TissueLyser II is not supplied with any accessories. TissueLyser Adapter Sets and Grinding Jar Sets need to be ordered separately (to order the TissueLyser II or its accessories, visit www.qiagen.com/Products/TissueLyserII.aspx). -
Page 30: Replacing The Fuses
Ensure that both adapters or grinding jars are of similar weight. Do not operate the TissueLyser II with only a single adapter or grinding jar installed. Replacing the fuses The fuses in the TissueLyser II can be replaced as follows: ... - Page 31 Figure 12. Backside of TissueLyser II. Power switch. Fuse compartment. Power cord socket. RS-232 port (for service personnel). Safety information plate. Voltage rating plate. Safety information plate. TissueLyser II User Manual 05/2016...
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Page 32: Operating Procedures
Operating Procedures This section describes how to operate the TissueLyser II system. Before proceeding, you should familiarize yourself with the features of the TissueLyser II (page 18) and have properly installed the instrument (page 26). Sample disruption The procedure below describes how to disrupt samples using the TissueLyser II. For detailed information about sample disruption and protocols, see the TissueLyser Handbook. -
Page 33: Storing Disruption Parameters
For the optimal disruption time for your application, see the TissueLyser Handbook. Press the “START” key on the keypad. The TissueLyser II will now operate at the frequency specified in step 5. In addition, the “Time min/sec” display will count down the disruption time (i.e., the display shows the time remaining). -
Page 34: Displaying The Accumulated Operating Time
TissueLyser II. Displaying the accumulated operating time To display the total amount of time that the TissueLyser II has been operated, follow the procedure below. When the TissueLyser II is in standby mode, simultaneously press the “SET” key and the “–”... -
Page 35: Maintenance Procedures
(see below). To remove residual cleaning agent and avoid instrument damage, the TissueLyser II should then be immediately wiped with a soft cloth moistened with water. Be sure to switch off the TissueLyser II at the power switch and unplug the power cord from the power socket before cleaning. -
Page 36: Cleaning Agents
QIAGEN Technical Services. Cleaning agents The following disinfectants and detergents are recommended for cleaning the TissueLyser II, TissueLyser Adapter Sets, and Grinding Jar Sets. Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described below. -
Page 37: Regular Safety Checks
TissueLyser II. Spray bottles should be used only for the TissueLyser Adapter Sets and Grinding Jar Sets. If solvents or saline, acidic, or alkaline solutions are spilt on the TissueLyser II, wipe the spilt liquid away immediately. Follow manufacturer's safety instructions for handling cleaning agents. - Page 38 TissueLyser Adapter Sets and Grinding Jar Sets may become worn, depending on the frequency of use and the samples disrupted. This may lead to damage to the clamps of the TissueLyser II. Check the TissueLyser Adapter Sets and Grinding Jar Sets regularly for wear and tear, and replace if necessary.
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Page 39: Troubleshooting Guide
“F04” displayed Transparent hood not lowered To protect the operator, the drive motor of the TissueLyser II will only start if the transparent hood has been lowered. If the “START” key is pressed while the hood is raised, “F04” is displayed and the drive motor does not start. If the hood is raised while the TissueLyser II is in operation, the drive motor switches itself off and “F04”... -
Page 40: Technical Data
Appendix A Technical data QIAGEN reserves the right to change specifications at any time. Environmental conditions Operating conditions Power 100–240 V AC, 50–60 Hz, 150 W Mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages Overvoltage... -
Page 41: Waste Electrical And Electronic Equipment (Weee)
Recycling can be provided by QIAGEN upon request at additional cost. In the European Union, in accordance with the specific WEEE recycling requirements and where a replacement product is being supplied by QIAGEN, free recycling of its WEEE-marked electronic equipment is provided. - Page 42 To recycle electronic equipment, contact your local QIAGEN sales office for the required return form. Once the form is submitted, you will be contacted by QIAGEN either to request follow-up information for scheduling collection of the electronic waste or to provide you with an individual quote.
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Page 43: Declaration Of Conformity
Declaration of Conformity Name and address of the company QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden Germany An up-to-date Declaration of Conformity can be requested from QIAGEN Technical Support. TissueLyser II User Manual 05/2016... -
Page 44: Appendix B
Representations and warranties made by any person, including representatives of QIAGEN, which are inconsistent or in conflict with the conditions in this warranty shall not be binding upon the Company unless produced in writing and approved by an officer of QIAGEN. -
Page 45: Notes
Notes TissueLyser II User Manual 05/2016... -
Page 46: Notes
Notes TissueLyser II User Manual 05/2016... - Page 47 (QIAGEN Group); DNA-ExitusPlus™ (AppliChem); Gigasept ® , Lysetol ® , Mikrozid ® (Schülke & Mayr GmbH); DECON-QUAT ® (Veltek Associates, Inc.); Teflon ® I. du Pont de Nemours and Company). HB-0262-004 © 2009–2016 QIAGEN, all rights reserved. TissueLyser II User Manual 05/2016...
- Page 48 Ordering www.qiagen.com/contact | Technical Support support.qiagen.com | Website www.qiagen.com TissueLyser II User Manual 05/2016 1102664...
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