Qiagen QIAcuity 911000 User Manual page 144

A 1D Scatterplot view has two axes. The x-axis represents the analyzed partitions, while the y-axis
represents the relative fluorescence intensity of each partition.
A 1D Scatterplot view concentrates the diagrams for each selected well in a horizontal way
separated by a vertical line (column indicator). For each well diagram a header is shown
indicating the well coordination on the plate. A red line represents the current threshold
fluorescence intensity value (decimal value) that is used to distinguish between positive and
negative partitions. Fluorescence values below the threshold are shown in grey, above the
threshold in blue color.
In one 1D Scatterplot at maximum seven well diagrams are shown on the page. The well diagrams
are scaled according to the number of wells shown on one page. If there are more than seven
selected wells, a horizontal scrollbar below the 1D Scatterplot is shown to the user to view the other
wells.
To view an additional toolbar that enables you to perform actions related to the diagram, such as
downloading the plot, zoom-in, zoom-out, hold the pointer over the diagram. For more details about
the toolbar, refer to Diagram options
The user can change the range of the y-axis by modifying the upper value of the fluorescence
intensity on the y-axis. For this the user clicks on top of the y-axis which opens a text box. This text
box is used to insert the new upper range of the fluorescence intensity. The view of the histogram
is adapted accordingly. Resetting the x-axis to the default range is provided by using the related
Reset Axes icon of the tool bar.
Changing the threshold individually per well
1. To change the threshold individually per well, click on the appropriate header of the well in
the 1D Scatterplot view. A window opens and the threshold can be changed by pointing over
the chart, which triggers the appearance of a dotted line.
2. Once the dotted line is in the appropriate spot, click the chart. The line becomes solid, and
the threshold value is updated and shown in the Threshold field.
3. To change the value again using this method, click the red line, and drag it to the appropriate
spot. Alternatively, you can also directly edit the value in the Threshold field Use the auto-
threshold button to set the threshold to the value, which is calculated by the analysis
algorithm.
Note: The Threshold field and the auto Threshold button become only visible by moving the
pointer in the range of the well diagram.
QIAcuity User Manual 07/2020 144