Qiagen QIAcuity 911000 User Manual page 146

The 1D Scatterplot view with Common threshold.
To add any of the 1D Scatterplot views to the report, click Add to report next to the relevant view.
For more information on reports, see .
Reference channel
The Software Suite also provides a 1D Scatterplot view for the reference channel, which is hidden
by default. To view the 1D Scatterplot for the reference channel in addition, click Show reference
channel. The title of this 1D Scatterplot view indicates that the 1D Scatterplot is related to the
reference channel. The graph for the reference channel provides the possibility to set a lower as
well as an upper threshold to exclude partitions with too high/ too low RFU. Setting the thresholds
(individually per well or common lower and upper threshold for all selected wells) is analog to 1D
Scatterplots for target channels. To apply the new threshold values for the analysis, click Recalculate.
The 1D Scatterplot for the reference channel uses the same functions as the 1D Scatterplot views for
non-reference channels.
QIAcuity User Manual 07/2020 146