Qiagen QIAcuity 911000 User Manual page 142

The Histogram tab.
To add any of the histograms to the report, click Add to report next to the relevant diagram. For
more information on reports, see section .Reference Channel
The Software Suite also provides a histogram for the reference channel, which is hidden by default.
To view the histogram for the reference channel in addition, click Show reference channel. The title
of this histogram indicates that the histogram is related to the reference channel. The graph for the
reference channel provides the possibility to set a common lower as well as upper threshold to
exclude partitions with too high/ too low RFU. Setting the upper threshold is analog to histograms
for target channels. Setting the lower threshold is only possible via chart. A dedicated text input
field for the lower threshold is not provided. If multiple source wells are selected, the values are
common for all wells. To apply the new threshold values for the analysis, click Recalculate to trigger
the recalculation of results.
QIAcuity User Manual 07/2020 142