Qiagen QIAcuity 911000 User Manual page 157

Each combination of wild type target and mutant target in one sample throughout the selected wells
is represented in the diagram with one bar showing the concentration value together with the
confidence interval. To view detailed information, hold the mouse pointer over its corresponding
bar. A tooltip with detailed information opens.
To view the mean values for replicates on the concentration diagram, click Show mean values for
replicates. If the user clicks into the checkbox to select the mean representation for replicates, the
bars of individual replicates disappear and only one bar is shown at the sample label that represents
the mean mutant fraction value of the replicates. When there are no replicates within selected wells
for mutant target the bars don't change. The corresponding well IDs of the replicates are shown on
the x axis. This is also applied in same way by default to the view where targets are in different
reaction mixes. In this case individual replicate results are not available. Mean values are
calculated and shown over all replicates that are included in the well selection only. If there are
further replicates of the same sample which are not included in well selection, they are not
considered in the calculated mean value and show their individual results values.
To add the concentration diagram to the report, click Add to report. For more information about
reports, see Reports.
QIAcuity User Manual 07/2020 157