Signal map of the dust-corrected image
In case the images still show dust particles or other particles after the correction, the
recommendation is to unload the plate, wipe the plate with a lint-free tissue and to re-image the
plate.
Low amplification areas
In case of low amplification areas, the fluorescence signal in the target channels is less pronounced
or not detectable in certain areas of the well. The signal in the reference channel is not affected. If
an equal amplification did not take place in the well, the area of low amplification does not meet
the requirements for Poisson distribution. Therefore, the partitions in those areas are blacked out in
the image and are not included in the analysis.
QIAcuity User Manual 07/2020
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