Qiagen QIAcuity 911000 User Manual page 164

2. Select the applicable reference sample from the Reference sample list.
3. Enter the number of copies per genome in the Copies/genome field. The value should be
between 1 and 99.
4. Select the applicable target that you want to investigate from the Target of interest list.
5. Select one or more reference targets from the Reference target(s) list.
Note: You can select more than one reference target.
6. To view the results of the analysis, click Show results.
The results are divided into several tabs. To view the contents of the tab, click the tab title.
List tab for copy number variation
The List tab contains a table with an overview of the analyzed wells. The following columns are
available in the table:
Well ID – This column represents the well position in the plate layout.

Sample/NTC/Control – This column shows the sample, NTC, or control name with its

corresponding icon that identifies the sample or indicates whether the entry is an NTC or
control. Reference samples are marked with the word Ref.
Reaction mix – This column contains the icon and the name of the Reaction mix

Target – This column shows all target names with its corresponding target type. Targets which

were selected as target of interest (TOI) or reference target (Ref) are marked accordingly.
Concentration (copies/ul) – This column shows the concentration assigned to each target or

channel.
CI (95%) – This column shows the value of the confidence interval at a 95% confidence level.

Copies/genome – This column shows the number of copies per genome in each of the targets

of interest.
QIAcuity User Manual 07/2020 164