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Qiagen QIAcube ht User Manual

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July 2013
®
QIAcube
HT User Manual
®
For QIAcube HT and QIAxtractor
Sample & Assay Technologies

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Summary of Contents for Qiagen QIAcube ht

  • Page 1 July 2013 ® QIAcube HT User Manual ® For QIAcube HT and QIAxtractor Sample & Assay Technologies...
  • Page 2 , Windows ® (Microsoft Corporation); Gigasept ® , Mikrozid ® , Lysetol ® (Schülke & Mayr GmbH); MagNA Pure ® , Roche ® (Hoffman-La Roche, Inc.); Teflon ® (E.I. du Pont de Nemours and Company). © 2009–2013 QIAGEN, all rights reserved.

  • Page 3: Table Of Contents

    2.2.2 Policy statement Intended use of the QIAcube HT 2.3.1 Requirements for QIAcube HT users 2.3.2 Requirements for using dedicated QIAcube HT kits on the QIAcube HT 2.3.3 Requirements for using dedicated QIAcube HT kits on the QIAxtractor 2.3.4 QIAcube HT Software...
  • Page 4 3.1.8 Worktable Principles of operation 3.2.1 Vacuum chamber and components for use with QIAcube HT kits on QIAcube HT or QIAxtractor instruments 3.2.2 Vacuum chamber and components for use with Capture Plate VX or Capture Plate DX on the QIAxtractor instrument 3-10 3.2.3...
  • Page 5 Configuring the COM port 4-18 Important notes before first use 4-19 QIAcube HT Software Mode 5.1.1 “Normal” mode 5.1.2 “Virtual” mode Starting the QIAcube HT Software The software workspace 5.3.1 “Virtual” mode indicator 5.3.2 Menu bar 5.3.3 Toolbar 5.3.4 Workspace items 5.3.5...
  • Page 6 5.14 Run files 5-89 5.15 Working with run files 5-90 5.15.1 Template protocol run files 5-90 5.15.2 Obtaining run files 5-91 5.15.3 Opening new or existing run files 5-91 5.15.4 Saving run files 5-93 Contents-4 QIAcube HT User Manual 07/2013...
  • Page 7 5.17.12 Generating filenames for reaction data export 5-126 5.18 Controlling communication and accessories 5-126 5.18.1 Selecting a COM port 5-126 5.18.2 Using the service register 5-127 5.18.3 Using the UV lamp 5-130 5.18.4 Using the HEPA filter 5-131 QIAcube HT User Manual 07/2013 Contents-5...
  • Page 8 Contents Operating Procedures Switching on the QIAcube HT Assembling the vacuum chamber 6.2.1 For plates from QIAcube HT kits 6.2.2 For Capture Plate VX or Capture Plate DX QIAcube HT protocols 6.3.1 Performing a protocol 6.3.2 Pre-run report 6-11 6.3.3...
  • Page 9 Safety Information (French, FR) Informations de sécurité Utilisation appropriée Sécurité électrique Sécurité biologique 1.3.1 Échantillons Produits chimiques 1.4.1 Vapeurs toxiques Elimination des déchets Dangers mécaniques Sécurité relative à la maintenance Symboles sur le QIAcube HT C-11 QIAcube HT User Manual 07/2013 Contents-7...
  • Page 10 Proben Chemikalien 1.4.1 Giftige Dämpfe Entsorgen von Abfällen Gefahren durch mechanische Teile Sicherheitshinweise – Wartungsarbeiten Symbole auf dem QIAcube HT D-11 Appendix E QIAcube HT and QIAxtractor accessories Appendix F Advanced settings Appendix G Sample and reaction functions for non-wizard protocols...

  • Page 11: Safety Information

    The QIAcube HT must only be operated by qualified personnel who have been appropriately trained. Servicing of the QIAcube HT must only be performed by QIAGEN Field Service Specialists. QIAcube HT User Manual 07/2013...
  • Page 12 WARNING/ Risk of personal injury and material damage [W3] CAUTION The QIAcube HT is too heavy to be lifted by one person. To avoid personal injury or damage to the instrument, do not lift the instrument alone. WARNING/ Risk of personal injury and material damage...

  • Page 13: Electrical Safety

    Never click any buttons while parts of your body are within the instrument workspace. In case of emergency, switch off the QIAcube HT at the power switch at the rear of the instrument and unplug the power cord from the power outlet.

  • Page 14: Biological Safety

    Safety Information To ensure satisfactory and safe operation of the QIAcube HT, follow the guidelines below: The line power cord must be connected to a line power  outlet that has a protective conductor (earth/ground). Do not adjust or replace internal parts of the instrument.

  • Page 15: Chemicals

    OSHA: Occupational Safety and Health Administration (United States of America). † ACGIH: American Conference of Government Industrial Hygienists (United States of America). ‡ COSHH: Control of Substances Hazardous to Health (United Kingdom). QIAcube HT User Manual 07/2013...

  • Page 16: Toxic Fumes

    For information on use and disposal of the tip disposal box, please see Section 3.2.6, page 3-17. For information about how to dispose of the QIAcube HT, see Appendix A. Mechanical hazards WARNING...

  • Page 17: Maintenance Safety

    Safety Information To ensure satisfactory and safe operation of the QIAcube HT, follow the guidelines below: Use only recommended tips and tip racks.  Keep the instrument lid closed whenever possible.  Do not override the lid sensor under any circumstances.
  • Page 18 Safety Information CAUTION Damage to the instrument [C4] Do not use solvents, or reagents containing acids, alkalis, or abrasives to clean the QIAcube HT. CAUTION Damage to the QIAcube HT lid [C5] Do not use alcohol, solvents, reagents containing acids, alkalis, or abrasives to clean the instrument lid.

  • Page 19: Symbols On The Qiacube Ht

    Safety Information Symbols on the QIAcube HT Symbol Location Description Worktable and near the Warning symbol type plate Worktable and near the Biohazard symbol tip chute outlet Worktable Squeezing warning symbol Type plate on the back CE mark for European Conformity...
  • Page 20 Symbol Location Description Type plate on the back RoHS mark for China (the restriction of the instrument of the use of certain hazardous substances in electrical and electronic equipment) Worktable Consult instructions for use 1-10 QIAcube HT User Manual 07/2013...

  • Page 21: Introduction

    Thank you for choosing the QIAcube HT. We are confident it will become an integral part of your laboratory. Before using the QIAcube HT, it is essential that you read this user manual carefully and pay particular attention to the safety information.

  • Page 22: General Information

    ® products. If you have any questions or experience any difficulties regarding the QIAcube HT or QIAGEN products in general, please do not hesitate to contact us. QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN.

  • Page 23: Requirements For Qiacube Ht Users

    Introduction The QIAcube HT is intended for use with QIAGEN kits indicated for use with the QIAcube HT for the applications described in the respective QIAGEN Kit handbooks. The QIAcube HT is intended for use by professional users, trained in molecular biological techniques and the operation of the QIAcube HT.

  • Page 24: Requirements For Using Dedicated Qiacube Ht Kits On The Qiacube Ht

    2.3.3 Requirements for using dedicated QIAcube HT kits on the QIAxtractor To process dedicated QIAcube HT kits on the QIAxtractor instrument, QIAcube HT Software version 4.17.1 or higher is needed together with the Accessories Pack, QXT (see Appendix E for ordering information).

  • Page 25: General Description

    8 samples. Innovative instrument features ensure reliable purification and minimize the risk of contamination. The QIAcube HT is a fully integrated solution and provides reagents, plasticware, and optimized protocols for nucleic acid purification from virtually any type of sample.

  • Page 26: The Qiacube Ht

    The QIAcube HT The QIAcube HT. Lysis plate UV lamp Filter plate and vacuum station Pipetting head Elution tubes (robotic arm) Tip chute HEPA filter Tip racks Accessory panel SBS tube (base plate) Feet Reagent troughs QIAcube HT User Manual 07/2013...

  • Page 27: Instrument Lid

    To operate the UV lamp: 1. Make sure that the UV lamp bulb is connected to the UV plug. 2. Make sure that the QIAcube HT instrument lid is securely closed. QIAcube HT User Manual 07/2013...

  • Page 28: Pipetting Head And Robotic Arm

    Under no circumstances should the user apply any force to the Y-arm. This may misalign the arm and cause pipetting errors. Note: To prevent accidental damage, the wooden Y-arm support should always be fitted before the QIAcube HT is moved. QIAcube HT User Manual 07/2013...

  • Page 29: Tip Chute

    Tip chute Tip chute. Tips used on the worktable are discarded through the tip chute. Located at the rear-left of the QIAcube HT, the tip chute is held in place by a locating block, allowing easy placement and removal for cleaning.

  • Page 30: Accessory Panel

    Worktable layout is specified for a protocol with a run file and is represented in the QIAcube HT Software as the workspace. For more details, see Section 5.3, page 5-6. QIAcube HT User Manual 07/2013...

  • Page 31: Principles Of Operation

    Riser block EMTR Vacuum chamber, channeling adapter, and riser block EMTR (unassembled). The QIAcube HT vacuum chamber is composed of two separate compartments for waste (left) and elution (right). This configuration is one of many measures that minimize sample cross-contamination during purification.
  • Page 32 The vacuum chamber hoses and the Y-hose connector are fitted to the instrument before delivery. For information about installation, see Section 4.4.3, page 4-4. Vacuum hoses must not be modified (e.g., shortened or lengthened). QIAcube HT User Manual 07/2013...
  • Page 33 The transfer carriage provides a platform for transporting the capture plate between the waste and elution compartments of the vacuum chamber. Transfer carriage for use with QIAGEN plates (e.g., QIAamp plate) other than Capture Plate VX or Capture Plate DX. QIAcube HT User Manual 07/2013...

  • Page 34: Vacuum Chamber And Components For Use With Capture Plate Vx Or Capture Plate Dx On The Qiaxtractor Instrument

    Vacuum chamber and components for use with Capture Plate VX or Capture Plate DX on the QIAxtractor instrument Note: This description is for QIAxtractor instruments only. All QIAcube HT instruments are delivered with the vacuum chamber components for dedicated QIAcube HT kits. Elution chamber Waste...
  • Page 35 The vacuum chamber hoses and the Y-hose connector are fitted to the instrument before delivery. For information about installation, see Section 4.4.3, page 4-4. Vacuum hoses must not be modified (e.g., shortened or lengthened). QIAcube HT User Manual 07/2013 3-11...
  • Page 36 Transfer carriage for use with Capture Plate VX or Capture Plate DX. CAUTION Damage to transfer carriage [C7] Under no circumstances should the transfer carriage for use with Capture Plate VX or Capture Plate DX be autoclaved. 3-12 QIAcube HT User Manual 07/2013...

  • Page 37: Vacuum Control Station And Waste Bottle

    Vacuum control station and waste bottle Vacuum control station. The vacuum control station, an integral component of the QIAcube HT, provides vacuum to the vacuum chamber for processing. The vacuum is precisely controlled by the user using the QIAcube HT Software.

  • Page 38: Pinch Valve

    General Description Vacuum bottle cap. 3.2.4 Pinch valve Pinch valve (base) and silicone hoses. Silicone hoses Pinch bar 3-14 QIAcube HT User Manual 07/2013...
  • Page 39 15-pin connector port on the front of the pinch valve. Pressing either button moves the pinch valve in the direction that the button is pushed, opening the vacuum to the opposite station. Pressing both buttons simultaneously QIAcube HT User Manual 07/2013 3-15...

  • Page 40: Reagent Trough Adapters And Reagent Troughs

    IMPORTANT: In case of protocol failure, do not push override during processing. 3.2.5 Reagent trough adapters and reagent troughs The QIAcube HT is delivered with 2 reagent trough adapters for securely positioning reagent troughs on the instrument worktable: The QIAcube HT specific reagent trough adapter is ...

  • Page 41: Tip Disposal Box

    Alignment of tip disposal box holder to the instrument and the tip disposal box. Tip disposal boxes are supplied with the QIAcube HT and can be reordered from QIAGEN. (See Appendix E for ordering information.)

  • Page 42: Qiacube Ht Laptop, Qiacube Ht Software Cd, And User Manual

    QIAcube HT and QIAcube HT Software is supplied as part of the QIAcube HT System. However, if a different computer is used to operate the QIAcube HT or to run the QIAcube HT Software, then the following desktop or laptop hosting systems are recommended.
  • Page 43 Note: The laptop that is supplied with the QIAcube HT supports RS-232 and USB connections. We recommend that the QIAcube HT be connected to the laptop via the RS-232 serial port. From time to time, QIAcube HT Software updates are released.
  • Page 44 General Description This page intentionally left blank 3-20 QIAcube HT User Manual 07/2013...

  • Page 45: Installation Procedures

    (earthed) AC power outlets. The power lines to the instrument should be voltage regulated and surge protected. The QIAcube HT can be installed on any level and sturdy  laboratory bench or table. There should be space below QIAcube HT User Manual 07/2013...

  • Page 46: Ac Power Connection

    The QIAcube HT operates at: 100–240 V, 250 VA, 50–60 Hz, 5 A fuse  Make sure the voltage rating of the QIAcube HT and laptop are compatible with the AC voltage available at the installation site. Main supply voltage fluctuations must not exceed 10% of nominal supply voltages.

  • Page 47: Hardware Installation

    Connect one end of the AC power cord (standard 3-wire IEC mains cable) to the power connector on the accessory panel located at the rear of the QIAcube HT, and the other end to the AC power outlet. The AC line fuse (slow blow) compartment is located on the accessory panel at the rear of the instrument between the power switch and the mains power input socket.

  • Page 48: Hepa Filter System

    (Section 3.1.6, page 3-6) and the female end into the accessory port on the vacuum control station. Side view of vacuum control station. 15-pin cable connector Restart Green LED (running) Accessory power port Red LED (standby) Power switch QIAcube HT User Manual 07/2013...

  • Page 49: Pinch Valve

    While pulling the hose down, use the other hand to reach through the pinch valve opening and push the hoses down through the gap between the rear cover and the pinch valve. QIAcube HT User Manual 07/2013...
  • Page 50 Y-hose connector. Note: If the instrument needs to be repacked for any reason, the Y-hose connector and vacuum chamber hoses should be stowed behind the rear cover to prevent damage. QIAcube HT User Manual 07/2013...

  • Page 51: Software Installation

    Note: Default settings are recommended where appropriate for all installation steps. 1. Turn on the laptop and close all open applications. 2. Insert the QIAcube HT Software CD into the DVD drive of the laptop. 3. The start page appears automatically after a few seconds.
  • Page 52 Make sure that Acrobat Reader is installed when using a computer other than the laptop delivered with the instrument. Adobe Acrobat Reader is required to allow viewing of the user manual (this document). QIAcube HT User Manual 07/2013...
  • Page 53 Adobe Reader. 6. Click “Install Operating Software” to begin the installation process. The “Welcome to the QIAcube HT Setup Wizard” window appears, informing the user of the software version and recommending that the user close all other programs before installing the software.
  • Page 54 Installation Procedures The “License Agreement” window displays the license agreement for the QIAcube HT Software. Return to the previous window by clicking “Back” in any window. 8. Read the license agreement, select “I accept the agreement”, and click “Next” to continue. Otherwise select “Cancel”...
  • Page 55 “Next” to continue. Click “Browse” to browse to the installation folder. Note: If a previous version of the software is installed, the “Select Destination Location” screen does not appear and the following warning is displayed. QIAcube HT User Manual 07/2013 4-11...
  • Page 56 This window is shown only on initial installation, not during installation of upgrades. After the region is selected the “Next” button becomes active. 11. Click “Next” to continue. 4-12 QIAcube HT User Manual 07/2013...
  • Page 57 Installation Procedures 12. In the “Select Additional Tasks” window, specify which additional tasks should be performed while the program is installing the software. 13. Click “Next” to continue. QIAcube HT User Manual 07/2013 4-13...
  • Page 58 14. Review the installation parameters in the “Ready to Install” window, and click “Install” to continue. The release notes for the software version are displayed. 15. Review the displayed release notes, and click “Next” to continue. 4-14 QIAcube HT User Manual 07/2013...

  • Page 59: Connecting The Instrument To The Laptop

     QIAcube HT Software has been installed (Section 4.5, page 4-7). The QIAcube HT has been properly set up and is in its  final location (Section 4.2, page 4-1). The laptop is in its final location near the instrument.

  • Page 60: Hardware Wizard

    QIAcube HT as a USB device even when the instrument is switched off. Switch on the instrument. Hardware Wizard The first time the QIAcube HT is connected to the laptop, the Windows “Found New Hardware” wizard appears. 1. Select “No, not this time”, and click “Next”. 4-16...
  • Page 61 2. Select “Install the software automatically (Recommended)”, and click “Next”. 3. Driver installation begins. Once installation is complete, the “Completing the Found New Hardware” window appears. 4. Click “Finish” to complete connection of the laptop to the instrument. QIAcube HT User Manual 07/2013 4-17...

  • Page 62: Configuring The Com Port

    Configuring the COM port The COM port used to connect the computer to the QIAcube HT must be correctly configured in the software to enable the software to communicate with the QIAcube HT instrument. By default, the software is configured to use COM1, and this corresponds to the first RS-232 port on the system.

  • Page 63: Important Notes Before First Use

    (see Section 5.18.1, page 5-126). Important notes before first use The QIAcube HT has a variety of moving parts which present a number of significant pinch points. For your own safety and to prevent damage to the instrument, please observe the...
  • Page 64 NEVER apply any force (by hand or otherwise) to the Y-arm.  Use “Stop/Pause” located in the toolbar of the  QIAcube HT Software to stop or pause the instrument before removing items from the worktable. 4-20 QIAcube HT User Manual 07/2013...

  • Page 65: Qiacube Ht Software

    QIAcube HT Software QIAcube HT Software The QIAcube HT Software provides a user-friendly, easy-to- use interface for configuring and running protocols on the QIAcube HT instrument. When started, the software automatically detects if the instrument is switched on and connected properly. The software displays a layout that corresponds to the setup of the instrument worktable.

  • Page 66: Mode

    QIAcube HT Software Mode The QIAcube HT Software can be run in either the “Normal” mode or the “Virtual” mode. 5.1.1 “Normal” mode When communication between the laptop and instrument has been correctly specified, the QIAcube HT Software opens automatically in “Normal” mode. This mode is used to operate the instrument.

  • Page 67: Starting The Qiacube Ht Software

    Section 6.1, page 6-1), and allow the instrument to initialize. 2. Switch on the laptop and wait for the Windows operating system to start up. 3. To start the software, double-click the QIAcube HT Software icon on the desktop. QIAcube HT QIAcube HT Software icon.
  • Page 68 Since the communication process takes several seconds, it is possible to start in “Virtual” mode by clicking “Virtual Mode” in the QIAcube HT window. After beginning operation in either mode, the software displays options for creating a new run or opening an existing run.
  • Page 69 When template protocols are installed, the form  offers a “Protocols” tab. To open a template run file, open the “Protocols” tab and select a file from the list of template run files (see Section 5.15.1, page 5-90). QIAcube HT User Manual 07/2013...

  • Page 70: The Software Workspace

    For more information about available options, see Section 5.15.3, page 5-91. The software workspace The software workspace is the main user interface providing control of QIAcube HT Software functions. The workspace is displayed: When a new project is initiated. ...
  • Page 71 QIAcube HT Software The software workspace displays positions that correspond to slots on the QIAcube HT worktable. Items specified in positions in the software workspace, referred to as “plates”, correspond to actual items placed on the worktable, and include the following: Multiwell plates (e.g., 96-well plates)

  • Page 72: Virtual" Mode Indicator

    5.3.2 Menu bar The menu bar provides access to the menus from which most software functions can be controlled. For more information about the options available from the menu bar, see Section 5.4, page 5-11. QIAcube HT User Manual 07/2013...

  • Page 73: Toolbar

    These buttons appear when the mouse pointer is hovering over a plate. The warning button is always visible for plates that have not yet been calibrated. For more information about calibration, see Section 5.12.5, page 5-66. QIAcube HT User Manual 07/2013...

  • Page 74: Right-Hand Pane

    Liquid-level tracking mode; double-click to toggle  Instrument model and COM port (when instrument is  connected) QIAcube HT Software version number; double-click to  review software and instrument details Individual operations being performed and error  conditions; double-click to review the diagnostic log Active user;...

  • Page 75: Menus

    – 1–8 List the last 8 run files that have been used. A run file can be selected from the list to open. – Exit (Ctrl+Q) Exit the QIAcube HT Software. QIAcube HT User Manual 07/2013 5-11...

  • Page 76: Control" Menu

    “Continue Run” or canceled by clicking “Cancel Run” in the window that appears. Extreme care should always be taken when pausing and opening the instrument lid during a run. 5-12 QIAcube HT User Manual 07/2013...

  • Page 77: Wizards" Menu

    (see Section 6.3.2, page 6-11). 5.4.4 “Options” menu The “Options” menu controls most instrument setup options, including configuration of run settings, calibration settings, and pipetting behavior. The following table describes the “Options” menu options. QIAcube HT User Manual 07/2013 5-13...
  • Page 78 Change Change the accessory assigned to the accessory selected plate. For more details, see Appendix G. Change plate Change the function of the selected plate. function For more details, see Section 5.8.4, page 5-28. 5-14 QIAcube HT User Manual 07/2013...

  • Page 79: Help" Menu

    “Help” menu The “Help” menu provides access to different documentation and diagnostic facilities which are listed in the following table. This item can help you to solve problems, sometimes under the guidance of a QIAGEN Technical Service Specialist. Menu item Description...
  • Page 80 QIAcube HT Software Identifying the instrument serial number Each QIAcube HT is identified by a unique serial number, which is displayed on the serial number badge at the rear of the instrument. The serial number is also stored electronically in the instrument, allowing the instrument to be identified by the software.

  • Page 81: Toolbar

    “About QIAcube HT…”. The version number is displayed above the tabs. This software version number is also displayed in the lower left region of the status bar in the QIAcube HT Software window (Section 5.3.6, page 5-10). Identifying the firmware version number All QIAcube HT instruments are identified with a firmware version number.
  • Page 82 The report can be exported, printed, or saved (see Section 6.3.2, page 6-11). Show help Display the QIAcube HT User Manual in PDF format. Adobe Acrobat Reader is necessary to view this document. 5-18...
  • Page 83 Enter comments that are saved with notebook… the run file. The comments are displayed when the run file is subsequently opened. This feature can be used as a type of laboratory notebook to document the purpose of the run. QIAcube HT User Manual 07/2013 5-19...
  • Page 84 5-131. Clean Display the “Instrument Cleaning vacuum Procedure” window. For more station… details, see Section 7.1, page 7-2. Open Starts the “Vacuum extraction” wizard… wizard. For more details, see Section 6.3.1, page 6-9. 5-20 QIAcube HT User Manual 07/2013...
  • Page 85 Section 5.15.1, page 5-90. Displays Opens a browser window to protocol display optional read-only information protocol details (when the loaded run is a template). For more details, see Section 5.15.1, page 5-90. QIAcube HT User Manual 07/2013 5-21...

  • Page 86: Right-Hand Pane

    The right-hand pane is at the right side of the software workspace. When the software is launched, the right-hand pane is typically gray and empty. Selecting a position in the software workspace displays information about the selected plate in the right-hand pane. 5-22 QIAcube HT User Manual 07/2013...

  • Page 87: Workspace Items

    5-23. Workspace items Software workspace items are software definitions that correspond to actual items placed on the QIAcube HT worktable (for more information about the workspace, see Section 5.3, page 5-6). Actual worktable items must be calibrated for use in protocols. To ensure proper functioning of the QIAcube HT, the correct workspace items matching the actual worktable items must be selected for each layout.

  • Page 88: Plates

    A “plate” is a workspace item (Section 5.7, page 5-23) that corresponds to an object placed on the QIAcube HT worktable in a slot or in the vacuum chamber. The following types of plates are used on the QIAcube HT: 96-well containers (e.g., sample, lysis, capture, and ...
  • Page 89 Plates with special functions, such as tip racks and reagent troughs, have additional properties, which are described in the following section and in Appendix G. QIAcube HT User Manual 07/2013 5-25...

  • Page 90: Choosing The Plate Type

    1. Open the software workspace (see Section 5.3, page 5-6). 2. Right-click the position of the plate to be chosen. 3. From the menu that appears, select “Change plate type”. The “Plate Configuration” window appears. 5-26 QIAcube HT User Manual 07/2013...

  • Page 91: Defining Favorite Plate Types

    QIAcube HT Software 4. To display plates that are not tip racks, select “QIAGEN” and “Other” in the “Filter to” panel. For more information about tip racks, see Section 5.9, page 5-29. 5. From the “Select Plate Type” drop-down list, select type of plates to be displayed in the “Plate”...

  • Page 92: Changing Plate Function

    “Remove Favorite”. 6. To add a new plate to the favorites list, click another “Filter to” option (such as “QIAGEN” or “Other”), choose the desired plate, and click “Add Favorite”. 7. After setting the “Filter to” options as desired and selecting a plate for the current position, click “OK”...

  • Page 93: Tips

    Accordingly, the software treats tips as special tubes that are placed in 96-well tip-rack plates. Tip workspace items are configured in the software using the same mechanisms that apply to conventional plates. A typical workspace representation of a tip-rack plate. QIAcube HT User Manual 07/2013 5-29...

  • Page 94: Tip Properties

    (Section 5.6, page 5-22). Right-hand pane information for a tip-rack plate. In addition to the properties that all plates have (see Section 5.8.1, page 5-24), tips have the properties listed in the following table. 5-30 QIAcube HT User Manual 07/2013...

  • Page 95: Tip Availability

    If the information is incorrect, the instrument may attempt to pick up a tip from a location where no tip is available and the run will not proceed correctly. QIAcube HT User Manual 07/2013 5-31...
  • Page 96 Used (dirty) tips — red (considered unavailable for  software calculations) Tip availability options are set with the tip operations menu, which is accessed by right-clicking a tip rack on the software workspace. Tip operations menu. 5-32 QIAcube HT User Manual 07/2013...
  • Page 97 “Set all tips on current plate to 'Unavailable'” “Set all tips on all plates to 'Available'” “Set all tips on all plates to 'Unavailable'” The color of the tip positions changes to indicate the change in status. QIAcube HT User Manual 07/2013 5-33...

  • Page 98: Reagents

    Each reagent normally performs a specific function in the process of sample purification, such as digestion, binding, or washing. In typical QIAcube HT protocols, reagents are defined in the following plate types (Section 5.8, page 5-24): Adapter, Reagent Trough SBS Plate ...
  • Page 99 Typically 5 µl for 20 µl PCR tubes and 0.35–7 ml for reagent troughs. As volume loss due to pipetting and evaporation can be substantial, this value should be reduced with care. QIAcube HT User Manual 07/2013 5-35...

  • Page 100: Configuring Reagents

    Reagents can be configured by completing the following steps: 1. Open the software workspace (see Section 5.3, page 5-6). 2. Right-click the position of the reagent to be specified. Initially, the reagent position in the software workspace appears gray. 5-36 QIAcube HT User Manual 07/2013...

  • Page 101: Required Reagent Volume

    The required reagent volume includes the volume of reagent dispensed, as well as any additional volume necessary for accurate liquid transfer (specified as “Extra Vol.”; also see Section 5.10.1, page 5-34). QIAcube HT User Manual 07/2013 5-37...

  • Page 102: Samples And Reactions

    When treated as a reaction, raw materials are configured as “preloaded” (for example, in a lysis plate) and other reagents may be added before further processing. 5-38 QIAcube HT User Manual 07/2013...

  • Page 103: Sample Properties

    (for example, the capture plate) during liquid transfer. 5.11.1 Sample properties Information about a selected sample segment on a plate (Appendix G) is displayed in the right-hand pane. QIAcube HT User Manual 07/2013 5-39...
  • Page 104 A sample bank is a selection of wells in a plate segment Name that is grouped and treated as a single unit for liquid- handling operations. In the QIAcube HT Software, sample banks are always aligned to the 8-channel pipettor.

  • Page 105: Reaction Properties

    Select a sample bank from the drop-down bank menu. Members of the selected bank are indicated in the grid view. 5.11.2 Reaction properties Information about a selected reaction segment on a plate (Appendix G) is displayed in the right-hand pane. QIAcube HT User Manual 07/2013 5-41...
  • Page 106 QIAcube HT Software 5-42 QIAcube HT User Manual 07/2013...
  • Page 107 Bar code See “ID” entry above. QIAcube HT User Manual 07/2013 5-43...
  • Page 108 Click o the new reaction plate to select it, click a reaction in the “Reaction List”, and click “Retarget”. Click “Yes” in the message that appears to continue. Displays a summary of the selected instruction. 5-44 QIAcube HT User Manual 07/2013...

  • Page 109: Common Properties And Operations

    Click the icon again to remove the highlight. 5.11.3 Common properties and operations In addition to identifiers for individual wells and concentration properties, the following properties and operations are common to sample and reaction segments: QIAcube HT User Manual 07/2013 5-45...
  • Page 110 This function is useful, for example, when it is convenient to distribute liquids by column (vertical pipet order), but to export the data by row (horizontal label order). 5-46 QIAcube HT User Manual 07/2013...

  • Page 111: Entering Sample Bar Codes

    (cursor) on the data table to the next input cell each time a bar code is read. 1. Click a “Sample” or “Reaction” plate to view the sample or reaction right-hand pane. 2. Click the icon. The “Bar code Entry” window opens. QIAcube HT User Manual 07/2013 5-47...

  • Page 112: Data Transfer Formats

    In the export mode where the data can be defined by the user, the user must specify the data content by writing an export string. The following table lists the data designators for export strings. 5-48 QIAcube HT User Manual 07/2013...
  • Page 113 – Sample name, excluding volume – Reagent name, excluding volume – Sample volume – Final concentration (if known) Plate identifier Destination plate identifier – Source plate identifier Table continued on next page. QIAcube HT User Manual 07/2013 5-49...

  • Page 114: Importing Sample And Reaction Data

    The copied entries are pasted to the QIAcube HT Software by right-clicking a cell and selecting the “Paste to current cell” item from the menu that appears. Sample names are copied to the target cells.
  • Page 115 Note: The software will automatically limit the number of rows imported to prevent overrunning the end of the import file and to prevent exceeding the available wells on the plate. QIAcube HT User Manual 07/2013 5-51...
  • Page 116 During the import, the wells to which data are imported are added to the selected sample bank. Existing members of the sample bank prior to import are retained unchanged. 5-52 QIAcube HT User Manual 07/2013...
  • Page 117 During import, only filtered rows displayed in the “Import Preview” pane are included in the imported data set. Two filters may be used. When used together, only rows matching both filtering criteria are included in the selected data set. QIAcube HT User Manual 07/2013 5-53...
  • Page 118 “Banks named from membership columns”. Note: Sample bank operations are disabled if the run has been set up using the “Vacuum extraction” wizard (see Appendix G). 5-54 QIAcube HT User Manual 07/2013...

  • Page 119: Exporting Sample And Reaction Data

    Exporting sample and reaction data Sample and reaction data, which is listed in the sample and reaction right-hand pane, can be exported in a number of formats. This can be used to transfer data to the QIAGEN QIAgility or Rotor-Gene ®...
  • Page 120 QIAcube HT Software To export sample or reaction data: 1. Click a sample or reaction plate to view the right-hand pane. 2. Click “Export”. The “Export Samples” or “Export Reaction Info” window appears. 5-56 QIAcube HT User Manual 07/2013...
  • Page 121 RotorGene A *.sam sample file which is compatible v4.4 with the Rotor-Gene 2000/3000 software version 4.6–5.x. The data are fixed and contains location, sample name, and sample type. QIAcube HT User Manual 07/2013 5-57...
  • Page 122 “sample identifier” (using the copy/paste substitution pattern %I as described in import file Section 5.11.5, page 5-48). This describes the contents of each well to facilitate export to the Roche MagNA Pure LC instrument. 5-58 QIAcube HT User Manual 07/2013...
  • Page 123 Determines the concentration value to be Option associated with the reaction for import into the Rotor-Gene Q software. Page Option Determines how the reaction data are to be grouped for analysis purposes after import into the Rotor-Gene Q software. QIAcube HT User Manual 07/2013 5-59...
  • Page 124 (user-assigned label) associated with the well. For each reaction identifier, a page is created and named according to the identifier. None All reactions are placed into a single page named “Page 1”. 5-60 QIAcube HT User Manual 07/2013...

  • Page 125: User Management

    7. Click “Save” to continue. 5.12 User management User management allows the definition of different access rights to various data and functions within the QIAcube HT Software. User management functions are accessed from the menu bar (“Options/User management”). 5.12.1...

  • Page 126: User Classes

    If required, standard Windows file system write-protection mechanisms may be used to protect run-file data. 5.12.2 User classes The QIAcube HT Software supports the following user groups: QIAcube HT Operator  QIAcube HT Designer ...
  • Page 127 “Manager”, “Designer” and “Operator” groups can operate the software. When attempting to start the QIAcube HT Software, users that are not assigned to a user groups are denied access to the software. QIAcube HT User Manual 07/2013...

  • Page 128: Enabling Access Control

    Upgrading the software does not affect user access control. 5.12.3 Enabling access control Upon installation, the QIAcube HT Software user access control features are disabled and all users have “Manager” access (see the previous section for user group descriptions). A Manager enables the user access control with “Options/User management/Enable access control”.
  • Page 129 QIAcube HT Software 4. Select the group to which the selected user should be assigned (i.e., “Administrators”, “QIAcube HT Manager”, “QIAcube HT Designer”, or “QIAcube HT Operator”) in the “Possible Groups” panel. 5. Click the button to move the selected group into the “Guest’s Groups:”...

  • Page 130: Change Current User

    Windows and restart the software. Alternatively, select “Options/User management/Change Current user”. The “Change User” window appears. The new user must enter their username and password, and click “OK” to log on. 5-66 QIAcube HT User Manual 07/2013...

  • Page 131: Revert To First User

    “calibration”. Volumes can also be calibrated to ensure the QIAcube HT distributes liquids accurately. The following calibrations are available: Plate position ...
  • Page 132 Care must be taken with some plates to ensure that correct orientation is maintained. Once the QIAcube HT has been correctly installed and calibrated, there is generally no need for recalibration. However, if the instrument is moved or serviced, we recommend that calibrations be checked and re-calibration performed if needed.

  • Page 133: Calibrating Plate Positions

    Calibrating plate positions Plate position calibration defines the positions of wells or tubes in a plate to be used on the QIAcube HT worktable. Calibration ensures that the robotic arm moves to the correct location for every well on the plate.
  • Page 134 The “Position Calibration” window appears. 6. If not calibrating a tip rack position, click “Get New Tip” to load tips onto the pipettor. If calibrating a tip rack position, use the pipettor hubs only without tips attached. 5-70 QIAcube HT User Manual 07/2013...
  • Page 135 (or contained consumable) indicated by the red well. Look at the robotic arm directly from both the front and the side to ensure correct alignment. The pipetting head may be lowered further to facilitate easier alignment. QIAcube HT User Manual 07/2013 5-71...

  • Page 136: Calibrating Plate Heights

    Calibrating plate heights Plate height calibration defines the base position of the wells of a plate to be used on the QIAcube HT worktable. Calibration allows software to correctly calculate the height at which tips must be placed for optimal aspirate and eject pipetting operations.
  • Page 137 Plate height warning Clicking the warning icon ( ) at the top, right corner of a plate that requires height calibration opens the “Configure Plate Heights” wizard, and the following warning. QIAcube HT User Manual 07/2013 5-73...
  • Page 138 (i.e., approximately 5 mm above the true base of the plate). Note: Height calibration data for QIAGEN capture plates are available within the QIAcube HT Software and normally do not require adjustment by the user. However, if height calibration is required, only the manual calibration method...
  • Page 139 The “Height Calibration” window appears. 7. From the “Plate Selection” list, select the type of plate to be calibrated by checking the corresponding boxes. Alternatively, you can select all plates by clicking “Select All”. QIAcube HT User Manual 07/2013 5-75...
  • Page 140 The instrument collects required tips and proceeds with automatic detection of heights in the wells or tubes to be measured. On completion, a notification about the height values determined is displayed. 11. Accept or reject the reported average. 5-76 QIAcube HT User Manual 07/2013...
  • Page 141 “Yes”. The “Height Calibration” window appears (see figure in the previous section). 7. From the “Plate Selection” list, select the type of plate to be calibrated manually by highlighting the corresponding text. QIAcube HT User Manual 07/2013 5-77...
  • Page 142 8. Click “Manual Setup”. A notification appears indicating which plate is to be manually calibrated. Click “Yes” to confirm. The instrument automatically collects tips and places the pipetting head above the plate to be calibrated. 5-78 QIAcube HT User Manual 07/2013...
  • Page 143 This step ensures greater accuracy. As it is not possible to see the distance between the tip and the base in most cases, the distance must be “felt” by manually lifting the well from its support. QIAcube HT User Manual 07/2013 5-79...

  • Page 144: Calibrating Volumes

    “Option 1” from the “Volume calibration” wizard. Before starting the first run on a newly installed QIAcube HT volume calibration has to be done. If no volume calibration has been done an error is shown at start of a run.
  • Page 145 Option 1 allows manual adjustment of the P-axis unit value for specific volumes. This method is used to enter the values supplied in the volume calibration report provided by QIAGEN. 1. Select “Option 1” from the “Volume calibration” wizard. The “Volume Calibration Wizard — Manual Calibration”...
  • Page 146 7. To update the default volume calibration with the displayed calibration settings, click “Use displayed settings as new calibration”. 8. If desired, click “Generate Report” to display the current calibration settings in table format. 5-82 QIAcube HT User Manual 07/2013...
  • Page 147 1. Select “Option 2” from the “Volume calibration” wizard. The “Volume Calibration Wizard — History of Calibration Data” window appears. 2. Proceed with step 6 of “Option 1” (page 5-82). QIAcube HT User Manual 07/2013 5-83...

  • Page 148: Calibration Management

    It is not necessary to use a setup disk when the software is updated to a new version. Note: QIAcube HT run files (e.g., “runfile.QSP”) are not instrument-specific and can be transferred between laptops connected to different instruments.
  • Page 149 1. From the “Options” menu in the menu bar, select “Calibration Management/Save calibration on setup disk...”. The “Calibration Settings” dialog box appears. Ensure that the displayed serial number matches the serial number of the connected instrument. 2. Click “Save” to continue. QIAcube HT User Manual 07/2013 5-85...

  • Page 150: Reinstating Calibration Data

    2. Specify the file name and location for the setup disk to be reinstated. 3. Click “OK” to reinstate the specified setup disk. 4. If calibration settings are found, the following window appears. Click “Yes” to overwrite the existing calibration settings. 5-86 QIAcube HT User Manual 07/2013...
  • Page 151 Click “Yes” (recommended). 6. If necessary, indicate whether the current run file should be saved. The calibration data will be reinstated when the QIAcube HT Software is restarted. QIAcube HT User Manual 07/2013 5-87...

  • Page 152: Deleting Calibration Data

    1. From the “Options” menu in the menu bar, select “Calibration Management/Delete multiple calibrations …”. The “Delete Multiple Calibrations” dialog box appears. 2. Select the type of data to be deleted. 3. Delete the data by clicking “Delete”. 5-88 QIAcube HT User Manual 07/2013...

  • Page 153: Run Files

    QIAcube HT Software 5.14 Run files QIAcube HT run files (file extension *.QSP) contain all the information required to perform a protocol on the QIAcube HT instrument. The run file specifies protocol settings and instrument-control parameters, including: Liquid transfer ...

  • Page 154: Working With Run Files

     “User Developed” template run files (  QProtocol run files are validated by QIAGEN, and optionally include read-only protocol information which is displayed when the run is loaded. The protocol information typically describes the processing of the run, kit details, and references to other material relevant for the protocol.

  • Page 155: Obtaining Run Files

    Opening new or existing run files Create a new run file or open an existing run file as follows: Select the “File/New” menu item or click the “New File…” toolbar icon to open a window for choosing options. QIAcube HT User Manual 07/2013 5-91...
  • Page 156 Use the “File/Open…” menu item or “Open file…”  toolbar item to open an existing run file. Browse to the desired file. Double-click or choose “Open” to open the chosen run file. 5-92 QIAcube HT User Manual 07/2013...

  • Page 157: Saving Run Files

    “File/Save As…” menu item or clicking the “Save file…” toolbar icon. Click “No” to discard the changes and proceed with the requested operation. Click “Cancel” to cancel the requested operation and return to working with the current modified run file. QIAcube HT User Manual 07/2013 5-93...

  • Page 158: Creating A Run File With The "Vacuum Extraction" Wizard

    All reaction steps existing prior to opening the wizard are discarded. 5-94 QIAcube HT User Manual 07/2013...

  • Page 159: Starting The "Vacuum Extraction" Wizard

    Using the “Open wizard…” toolbar item.  The first step of the “Vacuum extraction” wizard appears. 5.16.2 “Vacuum extractor configuration wizard” step The “Vacuum extractor configuration wizard” window allows configuration of parameters used in subsequent wizard steps and selections. QIAcube HT User Manual 07/2013 5-95...
  • Page 160 This option specifies the number of lysis reagents to be dispensed onto the sample by the instrument, prior to the loading the lysate onto the capture plate. The entered value determines the number of “Load Pre-Capture Reagent Steps” windows (Section 5.16.6, 5-96 QIAcube HT User Manual 07/2013...
  • Page 161 5. Select “Wet capture plate before loading sample?” to include a step that wets the capture plate before the lysed samples are loaded. This step is not usually required for standard nucleic acid purification. 6. Click “Next” to continue. QIAcube HT User Manual 07/2013 5-97...

  • Page 162: Table Setup" Step: Configuring The Layout

    (Section 5.3, page 5-6), including the items (reagent troughs, sample and lysis plates, capture plate, and tips) and their position on the instrument worktable. 1. After completing the “Vacuum extractor configuration wizard” step (previous section), the “Table setup” window appears. 5-98 QIAcube HT User Manual 07/2013...
  • Page 163 Note: Plate type must be correctly specified before its function can be determined. 5. Specify the plate function by completing the window that appears as described in Section 5.8.4, page 5-28. 6. Click “Next” to continue. QIAcube HT User Manual 07/2013 5-99...

  • Page 164: Configuration [1]" Step

    3. To track the plasticware pack, enter a value in the “Consumable lot number” field. Note: This is only necessary when “Tracking required for consumables and reagent pack lot numbers?” is activated (e.g., from an existing protocol template). 5-100 QIAcube HT User Manual 07/2013...

  • Page 165: Configuration [2]" Step

    “Configuration [2]” step appears. 2. For each specified reagent trough, select a size that is appropriate for processing 96 samples, and click the position to open a window for specifying reagent details. QIAcube HT User Manual 07/2013 5-101...
  • Page 166 70 ml reagent trough (cat. no. 990554) with the improved design. (The improved design has individual wells on the bottom of the trough to reduce the dead-volume). 5-102 QIAcube HT User Manual 07/2013...

  • Page 167: Load Pre-Capture Reagent 1" Step

    1. After completing the “Configuration [2]” step (previous section), the “Load Pre-Capture Reagent 1” window appears. Information in the “Load lysis” panel reflects the selections made in the “Vacuum Extractor Configuration Wizard” window (Section 5.16.2, page 5-95). QIAcube HT User Manual 07/2013 5-103...
  • Page 168 The working volume of a given plate is determined dynamically and not known until the wizard is completed. If the working volume is exceeded, a notification appears requiring the volumes to be adjusted accordingly before the protocol configuration can be finalized. 5-104 QIAcube HT User Manual 07/2013...

  • Page 169: Load Capture Plate" Step

    This step also allows vacuum processing to be specified. 1. After completing the “Load Pre-Capture Reagent” steps (previous section), the “Load capture plate” window appears. QIAcube HT User Manual 07/2013 5-105...

  • Page 170: Wash Step 1 To Wash Step N" Step

    “Vacuum extractor configuration wizard” step (Section 5.16.2, page 5-95). A similar window is shown for each wash reagent. Consecutive applications of the same wash reagent (iterations) are configured in the step for the wash. 5-106 QIAcube HT User Manual 07/2013...
  • Page 171 (15–25°C) on the capture plate before continuing, enter the incubation time. 4. Specify vacuum processing. For details, see Section 5.16.12, page 5-112. 5. Select the number of times the wash reagent should be applied (iterations). 6. Click “Next” to continue. QIAcube HT User Manual 07/2013 5-107...

  • Page 172: Dry Sample" Step

    The default is set at approximately 25 kPa for 5 minutes. The settings shown are for the DNA and virus protocols and are adequate for the DNA and virus capture plates. 3. Click “Next” to continue. 5-108 QIAcube HT User Manual 07/2013...

  • Page 173: Elution Steps" Step

    QIAcube HT Software 5.16.10 “Elution steps” step Before the elution steps are processed, the QIAcube HT moves the transfer carriage with the capture plate from the waste side of the vacuum chamber to the elution side of the vacuum chamber.
  • Page 174 TopElute fluid is added to the elution buffer then vacuum is applied. TopElute fluid only partially passes the membrane thereby preventing the flow of air through empty wells. 8. Click “Next” to continue. 5-110 QIAcube HT User Manual 07/2013...

  • Page 175: Wizard Summary

    The following options are available for the report. “Print”: Print the report  “Save”: Save the report in HTML format  “Email”: Save the report as an e-mail attachment  “MSWord”: Save the report as a Microsoft Word  document QIAcube HT User Manual 07/2013 5-111...

  • Page 176: Specifying Vacuum Processing

    Target vacuum pressure is specified in kilopascal (kPa) in the “Vacuum pressure” field. The optimal vacuum pressure depends on a number of factors. The viscosity and nucleic acid concentration of the sample as well as the amount of protein and other non- 5-112 QIAcube HT User Manual 07/2013...
  • Page 177 This is useful only during drying operations, and is only displayed for contexts where drying is appropriate. QIAcube HT User Manual 07/2013 5-113...

  • Page 178: Setting Run Parameters

    We recommend 0 µl as the air intake volume for QIAcube HT applications. Configure air intake volumes as follows: 1. From the “Options” menu in the menu bar, select “Run Settings/Configure tip air intake…”. The “Configure tip air intake” dialog box appears. 5-114 QIAcube HT User Manual 07/2013...

  • Page 179: Configuring Pipet Speed

    Slower speeds can be chosen for pipetting viscous liquids. When specifying reagent troughs for a layout, the reagent can be defined as “normal” or “viscous” (see Section 5.9, page 5-29). QIAcube HT User Manual 07/2013 5-115...

  • Page 180: Using Liquid-Level Tracking

    Settings/Use liquid level when pipetting?”. 2. Select an option from the menu that appears: Do not use liquid level.  Tip is placed at the bottom of the plate or reagent trough for aspirating and dispensing tasks. 5-116 QIAcube HT User Manual 07/2013...

  • Page 181: Configuring Error Reporting

    QIAcube HT Software Detect liquid level using sensor.  Function is not available on the QIAcube HT and selection is inactive. Use liquid level estimate.  Default. Software tracks liquid transfer and calculates volumes present in plates or reagent troughs,...

  • Page 182: Setting Tip Use Options

    This option uses a simple optimization strategy to reuse tips where possible. IMPORTANT: When using this option, ensure that plates containing liquid are assigned a non-zero preload volume (right-click the plate to select and set 5-118 QIAcube HT User Manual 07/2013...

  • Page 183: Setting The Default File Location

    Set file location to the default as follows: 1. From the “Options” menu in the menu bar, select “Run Settings/Set default file location…?”. A dialog box for browsing to the folder appears. QIAcube HT User Manual 07/2013 5-119...

  • Page 184: Resetting To Default Values

    “Options” menu to reset to the original preinstalled default. Reset to original defaults as follows: 1. From the “Options” menu in the menu bar, select “Run Settings/Reset settings to factory defaults?”. 5-120 QIAcube HT User Manual 07/2013...

  • Page 185: Saving Current Settings As New Defaults

    To save current settings as new defaults, ensure that run settings in the “Options” menu are set as desired, and select the “Run Settings/Save current settings as new defaults?” option. To restore original preinstalled defaults for all settings, see Section 5.17.7, page 5-120. QIAcube HT User Manual 07/2013 5-121...

  • Page 186: Setting System Color Configuration

    The “System color configuration” option allows system color configuration to be modified according to preference. From the “Options” menu in the menu bar, select “Run Settings/System color configuration”. The “Sample Color Configuration” dialog box appears. 5-122 QIAcube HT User Manual 07/2013...
  • Page 187 Refresh the colors displayed on the software worktable according to the current settings. Open color theme. Save color theme. Apply changes and close. Open the “Sample Colors” tab, and select colors used for samples. QIAcube HT User Manual 07/2013 5-123...
  • Page 188 Open the “Liquid Colors” tab, and select colors used for reagents and other liquids. Open the “Selection Colors” tab and select colors used for liquid functions and for representation of members of a sample bank, as well as for selection and highlighting. 5-124 QIAcube HT User Manual 07/2013...

  • Page 189: Setting Shutdown Beep

    By default, the software inhibits the operation of screensavers to ensure that the user interface is always available while the QIAcube HT Software is running. From the “Options” menu in the menu bar, select “Run Settings/Allow screensaver?” to enable and disable the screen saver.

  • Page 190: Generating Filenames For Reaction Data Export

    “Virtual” mode (see Section 5.1.2, page 5-2). Change the COM port manually as follows: 1. Run the QIAcube HT Software in the “Virtual” mode. 2. From the “Options” menu in the menu bar, select “Robot Setup/COM port”.

  • Page 191: Using The Service Register

    1. From the “Options” menu in the menu bar, select “Robot Setup/Service register”. The “Service Register” dialog box appears. The component that requires service most urgently is selected (i.e., “HEPA Filter” in the figure above). QIAcube HT User Manual 07/2013 5-127...
  • Page 192 Clicking “Exit” closes the dialog box. 2. To acknowledge a service, select the component that has been serviced. 3. Enter your user name in the “Acknowledged By” field. 5-128 QIAcube HT User Manual 07/2013...
  • Page 193 Register” dialog box. Note: Volume calibration must be done before starting the first run after acknowledging a pipettor service. If no volume calibration has been done, an error is shown at the start of a run. QIAcube HT User Manual 07/2013 5-129...

  • Page 194: Using The Uv Lamp

    4. Specify the irradiation. For manual control: Click “Enable Lamp” to start  irradiation. To end irradiation, click “Disable Lamp”. Note: Under manual control, the robotic arm does not move and shadowing results. 5-130 QIAcube HT User Manual 07/2013...

  • Page 195: Using The Hepa Filter

    For more information about the HEPA filter see Section 4.4.2, page 4-3. Turn the HEPA filter on or off as follows: 1. Ensure the HEPA filter is connected to the QIAcube HT with the accessory cable before switching on the instrument and initializing the software.
  • Page 196 QIAcube HT Software This page intentionally left blank 5-132 QIAcube HT User Manual 07/2013...

  • Page 197: Operating Procedures

    3. Switch on the connected laptop and wait for the operating system to complete startup. 4. Start the QIAcube HT Software (see Section 5.2, page 5- 5. If the software opens in the “Normal” mode (see Section 5.1, page 5-1), the QIAcube HT can be used.

  • Page 198: Assembling The Vacuum Chamber

    Assembling the vacuum chamber 6.2.1 For plates from QIAcube HT kits All QIAcube HT instruments are delivered with the vacuum chamber components for dedicated QIAcube HT kits. To upgrade your QIAxtractor instrument to process the dedicated QIAcube HT kits, QIAcube HT Software version 4.17.1 or higher and the Accessories Pack, QXT are required...
  • Page 199 7. Place the riser block EMTR in the right (elution) side of the vacuum chamber with the pin of the riser block EMTR in the right top position. 8. Load an elution microtubes rack (EMTR) into the elution chamber. QIAcube HT User Manual 07/2013...

  • Page 200: For Capture Plate Vx Or Capture Plate Dx

    For Capture Plate VX or Capture Plate DX Note: This description is for QIAxtractor instruments only. All QIAcube HT instruments are delivered with the vacuum chamber components for dedicated QIAcube HT kits. The following figure illustrates the assembly of the vacuum chamber.
  • Page 201 6. Check the alignment by looking through the hole in the waste sink/mat support to the silicone mat. When properly aligned, 4 mat holes should be visible. QIAcube HT User Manual 07/2013...
  • Page 202 11. Check of the sealing lip of the capture plate to be sure there are no chips or cracks. Chips, cracks, and anything else that interferes with a good seal will reduce the vacuum efficiency. QIAcube HT User Manual 07/2013...
  • Page 203 15. Ensure the elution riser block is in the right (elution) side of the vacuum chamber. 16. Load a covered elution plate into the elution chamber. Squeeze the locking tabs on the elution plate lid and remove the lid. QIAcube HT User Manual 07/2013...

  • Page 204: Qiacube Ht Protocols

    For more information about run files, see Section 5.14, page 5-89. To process dedicated QIAcube HT kits on the QIAxtractor instrument, QIAcube HT Software version 4.17.1 or higher is needed together with the Accessories Pack, QXT (see Appendix E for ordering information).

  • Page 205: Performing A Protocol

    (e.g., ensure that the UV cover has been removed). 2. Switch on the QIAcube HT instrument and the connected laptop. We recommend leaving the vacuum station switched on at all times.
  • Page 206 14. Ensure that the vacuum station liquid waste bottle is no more than half full to prevent the risk of filter blockage during the run. For more information, see Section 7.1.1, page 7-2. 6-10 QIAcube HT User Manual 07/2013...

  • Page 207: Pre-Run Report

    When performing a protocol, a pre-run report can be prepared from the pre-run check (see Section 6.3.1, page 6-9) from the “Wizards/Generate Report...” menu item, or by using the “Generate pre-run report…” toolbar item. QIAcube HT User Manual 07/2013 6-11...
  • Page 208 Operating Procedures 6-12 QIAcube HT User Manual 07/2013...
  • Page 209 The pre-run report contains the following information: Name of the run file  Name of the user performing the protocol  Version of QIAcube HT Software  Mode (“Normal” or “Virtual”) in which the instrument is  running The instrument type and serial number, when operating ...

  • Page 210: Pre-Run Check Errors And Warnings

    “Operation Impossible” — a task (or combination of  tasks) in the reaction list cannot be performed “Bad Plate Config” — plate configuration can not be  used For system error messages and codes, see Section 8.2, page 8-1. 6-14 QIAcube HT User Manual 07/2013...

  • Page 211: In-Run Open System/Open Circuit Test

    For every protocol run that uses the vacuum pump, a “Plate Detection” test is performed as the second step of the run. It follows immediately after the “Open Circuit” test. This step is implicit in the run and cannot be removed or skipped. The QIAcube HT User Manual 07/2013 6-15...

  • Page 212: In-Run Interactive Vacuum Steps

    (where possible). Buttons in the dialog box allow the run to be canceled (“Cancel run”) or the operation to be 6-16 QIAcube HT User Manual 07/2013...
  • Page 213 Where user confirmation is required for the step to complete, the dialog box changes its presentation. A “Continue” button is shown instead of an “Advance” button, and a different visual alert appears. An audible alert QIAcube HT User Manual 07/2013 6-17...
  • Page 214 If “Continue” is selected, a new window appears with the choice either to resume the vacuum or advance to proceed with the protocol. If “Resume” is selected, the vacuum turns on until the “Continue” button is clicked. 6-18 QIAcube HT User Manual 07/2013...

  • Page 215: Modifying A Protocol Reaction List

    (e.g., capture plate) is selected. This can be useful to assist in recovering an interrupted run that has not fully completed because of an error. QIAcube HT User Manual 07/2013 6-19...

  • Page 216: Canceling A Protocol

    To pause a run, click in the toolbar. The instrument will complete its current operation (e.g., it will never pause when the pipetting head is at a position below the resting position and pause. 6-20 QIAcube HT User Manual 07/2013...

  • Page 217: Post-Run Report

    Note: Once canceled, a run cannot be restarted from the canceled step. Post-run report At the completion of a run, a post-run report is generated by the software. The report is similar in presentation to the pre-run report (Section 6.3.2, page 6-11). QIAcube HT User Manual 07/2013 6-21...
  • Page 218  Name of the user who performed the protocol  Name of the report generated  Version of QIAcube HT Software  Mode (“Normal” or “Virtual”) in which the instrument  was running The instrument type and serial number, when operating ...

  • Page 219: Maintenance Procedures

    Support Agreements from QIAGEN. Annual preventive maintenance QIAGEN recommends that an annual preventive maintenance service is carried out on the QIAcube HT. The service should be performed by a trained QIAGEN Field Service Specialist. Contact your local QIAGEN Field Service Specialist or your local distributor for more information.

  • Page 220: Regular Maintenance Procedures

    “bottle full” alert. It is possible to empty the waste bottle at other times without waiting for a warning. To reset the indicator, click in the toolbar, and click “Yes” in the window that appears. QIAcube HT User Manual 07/2013...

  • Page 221: Cleaning The Qiacube Ht

    Cleaning the QIAcube HT CAUTION Damage to the instrument [C4] Do not use solvents, or reagents containing acids, alkalis, or abrasives to clean the QIAcube HT. CAUTION Damage to the QIAcube HT lid [C5] Do not use alcohol, solvents, reagents containing acids, alkalis, or abrasives to clean the instrument lid.
  • Page 222 Only perform maintenance that is specifically described in this user manual. WARNING Risk of electric shock [W14] Do not open any panels on the QIAcube HT. Risk of personal injury and material damage Only perform maintenance that is specifically described in this user manual. Cleaning agents The following disinfectants and detergents are recommended for cleaning the QIAcube HT.
  • Page 223 If solvents or saline, acidic, or alkaline solutions are spilt  on the QIAcube HT or if QIAGEN buffers splash the instrument lid, wipe the spilt liquid away immediately. Follow manufacturer's safety instruction for handling ...

  • Page 224: Cleaning The Instrument After Completing A Run

    Maintenance Procedures Do not use alcohol or alcohol-based disinfectants to  clean the QIAcube HT lid. Exposing the QIAcube HT lid to alcohol or alcohol-based disinfectants will cause surface cracking. Clean the QIAcube HT lid with distilled water only. Wipe the instrument with a damp cloth only.

  • Page 225: Cleaning The Instrument After Shutting Down

    6. Remove the carriage and discard the capture plate. Clean carriage according to instructions in Section 7.1.5, page 7-8. 7. If using plates from dedicated QIAcube HT kits, leave the channeling adapter in position until the waste chamber has been flushed.

  • Page 226: Cleaning Instrument Worktable Accessories

    The instrument lid should be cleaned only with water and a clean, soft cloth. Alternatively, a cleaning product specifically designed to clean acrylics can be used. Any cleaning product QIAcube HT User Manual 07/2013...
  • Page 227 Greasing the O-ring If using plates from dedicated QIAcube HT kits, apply a thin film of vacuum grease to the O-ring, as required, on the channeling block holder indicated with a red arrow in the following figure).
  • Page 228 Maintenance Procedures Waste sink/mat support O-ring. Cleaning air bleed holes The QIAcube HT and QIAxtractor instruments have air bleed holes positioned at strategic points on the vacuum chamber and the waste sink/mat support, as shown in the following figures. Each of these air bleeds has a small stainless steel aperture inserted in the hole to specifically control the rate of air flow.

  • Page 229: Weekly Maintenance Procedure

    The linear bearings incorporate seals to keep the bearings free of dust or grit. The rails and bearings have been tested on the QIAcube HT in excess of 2,000,000 operations without any sign of degradation. Weekly maintenance procedure...
  • Page 230 9. Rinse the workspace with 70% ethanol, and allow to air dry. 10. Replace all components on the worktable. 11. Close instrument lid. 12. Switch on the UV lamp for 15 min. For more details, see Section 5.18, page 5-126. 7-12 QIAcube HT User Manual 07/2013...

  • Page 231: Pipetting Head Service Kit

    3 months for 3 or more plates per day. Replacing HEPA filters HEPA filters should be replaced on a regular basis. The QIAcube HT Software reminds you to replace the HEPA filters at 6 monthly intervals; replacement should take place then. Replace the HEPA filters as follows: 1.

  • Page 232: Replacing Filters On The Vacuum Station

    Replacing filters on the vacuum station Vacuum filters should be replaced on a regular basis. The QIAcube HT Software reminds you to replace these filters at 6 monthly intervals; replacement should take place then. Replace vacuum filters as follows: 1.

  • Page 233: Shutting Down The Qiacube Ht

    Shutting down the QIAcube HT The QIAcube HT Software can be shut down by clicking the top right hand corner of the QIAcube HT window or by selecting “Exit” in the “File” menu. During shut down, the user is prompted to clean the instrument.
  • Page 234 Maintenance Procedures This page intentionally left blank 7-16 QIAcube HT User Manual 07/2013...

  • Page 235: Troubleshooting

    QIAGEN Technical Services. If the support package cannot be saved because of a communication problem, start the software with the instrument powered down, save the support package immediately, and then contact QIAGEN Technical Services.

  • Page 236: Software Errors

    Instrument error numbers begin with 40000. Specific error codes from the instrument are reported as the sum of this base value (40000) and the code listed in the following table. The following table lists selected instrument errors. QIAcube HT User Manual 07/2013...

  • Page 237: Error Codes

    Error reading accessory EEPROM. Contact QIAGEN Technical Services. An invalid axis was specified. Turn the power off/on and restart. If the problem persists, contact QIAGEN Technical Services. The instrument did not respond with the first reply. Contact QIAGEN Technical Services Table continued on next page.
  • Page 238 More data was received while processing. Turn the power off/on and restart. If the problem persists, contact QIAGEN Technical Services. If any of these errors are encountered, contact QIAGEN Technical Services with a support package that contains the event where the error occurred.

  • Page 239: Technical Data

    Appendix A Appendix A Technical data QIAGEN reserves the right to change specifications at any time. This technical data applies to QIAxtractor and QIAcube HT instruments and vacuum stations. All instruments are marked on the type plate as QIAxtractor. Environmental conditions...

  • Page 240: Mechanical Data And Hardware Features

    QIAcube HT vacuum station Width: 495 mm (19 in.) Height: 520 mm (20 in.) Depth: 400 mm (16 in.) Weight QIAcube HT instrument: 44 kg (97 lb) QIAcube HT vacuum control station: 19 kg (42 lb.) QIAcube HT User Manual 07/2013...
  • Page 241 Instrument lid Material Acrylic (SMMA)/Polyester (UP) Work surface UV lamp attachment sterilization The UV light is connected by the QIAGEN Field Service Engineer when the instrument is installed (see Section 4.4.1 for details) HEPA filter unit Positive Preinstalled HEPA filter unit...

  • Page 242: Hardware And Software Features

    Appendix A Hardware and software features Laptop The QIAcube HT is operated with a connected laptop.  and software A laptop appropriate for the correct operation of the QIAcube HT Software is supplied as part of the QIAcube HT system.
  • Page 243 Appendix A Uninstalling the The QIAcube HT Software can be uninstalled from the QIAcube HT “Settings” or the “Programs” menu. Software To uninstall the software from the “Settings” menu: From the Windows “Start” menu, select  “Settings/Control Panel”. The “Control Panel” opens.

  • Page 244: Waste Electrical And Electronic Equipment (Weee

    Recycling can be provided by QIAGEN upon request at additional cost. In the European Union, in accordance with specific WEEE recycling requirements, and where a...

  • Page 245: Fcc Declaration

    QIAGEN GmbH Germany is not responsible for any radio television interference caused by unauthorized modifications of this equipment or the substitution or attachment of connection cables and equipment other than those specified by QIAGEN GmbH, Germany.

  • Page 246: Declaration Of Conformity

    Electromagnetic Compatibility 2004/108/EC Directive (EMC) and the relevant harmonized standards IEC 61010-1 (Ed.2) IEC 61010-2-081 (Ed.1) IEC 61326-1 Accessories Vacuum Station Pinch Valve Hombrechtikon, 3 August 2009 Pit Muggli Director Business Excellence QIAGEN Instruments AG QIAcube HT User Manual 07/2013...

  • Page 247: Liability Clause

    Appendix B Appendix B Liability clause QIAGEN shall be released from all obligations under its warranty in the event repairs or modifications are made by persons other than its own personnel, except in cases where the Company has given its written consent to perform such repairs or modifications.
  • Page 248 Appendix B This page intentionally left blank QIAcube HT User Manual 07/2013...

  • Page 249: Safety Information (French, Fr

    Appendix C Safety Information (French, FR) Informations de sécurité Avant d'utiliser le QIAcube HT, il est impératif de lire attentivement ce manuel et de porter une attention particulière aux informations de sécurité. Afin de garantir un fonctionnement de l'appareil en toute sécurité et de maintenir l'appareil en bon état de marche, il est impératif de suivre les...

  • Page 250: Utilisation Appropriée

    AVERTISSEMENT/ Risque d’accident corporel et de détérioration ATTENTION du matériel [W3] Le QIAcube HT est trop lourd pour être soulevé par une seule personne. Afin d'éviter tout accident corporel et toute détérioration du matériel, ne pas soulever l'appareil seul. AVERTISSEMENT/ Risque d’accident corporel et de détérioration...
  • Page 251 ATTENTION Détérioration de l’appareil [C1] La lumière directe du soleil peut décolorer les pièces de l'appareil et abîmer les éléments en plastique. Tenir le QIAcube HT à l'abri de la lumière directe du soleil. ATTENTION Détérioration de l’appareil [C2] Éviter de renverser de l’eau ou des produits chimiques sur le QIAcube HT.

  • Page 252: Sécurité Électrique

    éléments sous tension. AVERTISSEMENT Danger d'irradiation UV [W9] Un dispositif électronique de verrouillage empêche la lampe UV de fonctionner tant que le capot de l'appareil est ouvert. Ce dispositif ne doit en aucun cas être désactivé. QIAcube HT User Manual 07/2013...

  • Page 253: Sécurité Biologique

    Appendix C Afin que le QIAcube HT fonctionne de manière satisfaisante et en toute sécurité, suivre les conseils suivants : Le cordon d'alimentation de l'appareil doit être branché  sur une prise d'alimentation munie d’un conducteur de protection (terre/masse). Ne pas effectuer de réglage, ni remplacer les pièces ...
  • Page 254 † ACGIH : American Conference of Government Industrial Hygienists (États-Unis d’Amérique) (Conférence américaine des hygiénistes industriels gouvernementaux). ‡ COSHH : Control of Substances Hazardous to Health (Royaume-Uni) (Contrôle des substances dangereuses pour la santé). QIAcube HT User Manual 07/2013...

  • Page 255: Produits Chimiques

    † ACGIH : American Conference of Government Industrial Hygienists (États-Unis d’Amérique) (Conférence américaine des hygiénistes industriels gouvernementaux). ‡ COSHH : Control of Substances Hazardous to Health (Royaume-Uni) (Contrôle des substances dangereuses pour la santé). QIAcube HT User Manual 07/2013...

  • Page 256: Vapeurs Toxiques

    AVERTISSEMENT Pièces mobiles [W12] Éviter le contact avec les pièces en mouvement lorsque le QIAcube HT est en fonctionnement. Ne jamais placer les mains sous le bras robotisé lorsque celui-ci s'abaisse. Ne pas tenter de déplacer des plaques ou des tubes lorsque le bras robotisé est en marche.

  • Page 257: Sécurité Relative À La Maintenance

    Appendix C Afin que le QIAcube HT fonctionne de manière satisfaisante et en toute sécurité, suivre les conseils suivants : Utiliser uniquement les cônes et les supports de cônes  recommandés. Dans la mesure du possible, maintenir le capot de ...
  • Page 258 Ne pas utiliser de solvants ni de réactifs contenant des acides, des bases ou des composés abrasifs pour nettoyer le QIAcube HT. ATTENTION Détérioration du capot du QIAcube HT [C5] Ne pas utiliser de solvants ni de réactifs contenant des acides, des bases ou des composés abrasifs pour nettoyer le capot de l'appareil.

  • Page 259: Symboles Sur Le Qiacube Ht

    Appendix C Symboles sur le QIAcube HT Symbole Emplacement Description Table de travail et près Symbole de mise en garde de la plaque signalétique Table de travail et près Symbole de danger biologique de la sortie pour l’éjection des cônes Table de travail Symbole de risque d'écrasement...
  • Page 260 Appendix C Symbole Emplacement Description Plaque signalétique à Label RoHS pour la Chine l’arrière de l’appareil (restriction de l’utilisation de certaines substances dangereuses dans l’équipement électrique et électronique) Table de travail Lire le manuel d’utilisation. C-12 QIAcube HT User Manual 07/2013...

  • Page 261: Safety Information (German, De

    Safety Information (German, DE) Sicherheitshinweise Lesen Sie dieses Handbuch sorgfältig durch, bevor Sie den QIAcube HT benutzen. Beachten Sie dabei insbesondere die Sicherheitshinweise. Die Gebrauchsanweisungen und Sicherheitshinweise im Handbuch müssen befolgt werden, um einen sicheren Betrieb des Geräts zu gewährleisten und das Gerät in einem sicheren Zustand zu erhalten.

  • Page 262: Sachgemäße Handhabung

    WARNUNG/ Verletzungsgefahr und Beschädigung des Geräts [W3] ACHTUNG Der QIAcube HT ist zu schwer, um von einer einzelnen Person gehoben zu werden. Zur Vermeidung einer Verletzung oder Beschädigung des Gerätes heben sie das Gerät nicht alleine an. WARNUNG/ Verletzungsgefahr und Beschädigung des Geräts...

  • Page 263: Schutz Vor Stromschlag

    Maus und drücken Sie nicht auf eine Taste der Tastatur, während sich Ihre Hände oder andere Körperteile im Arbeitsbereich des Roboterarms befinden. Schalten Sie im Notfall den QIAcube HT aus (der Netzschalter befindet sich auf der Geräterückseite), und ziehen Sie den Netzstecker aus der Steckdose.
  • Page 264 Gerätehaube geschlossen sein muss, bevor die UV-Lampe eingeschaltet werden kann. Der Hauben Sensor darf unter keinen Umständen ausser Kraft gesetzt werden. Um einen zufriedenstellenden und sicheren Betrieb des QIAcube HT zu gewährleisten, befolgenden Sie bitte die nachstehenden Richtlinien: Das Netzkabel muss an eine Wechselstrom-Steckdose ...

  • Page 265: Biologische Sicherheit

     Geräts nicht mehr gewährleistet werden kann, muss das Gerät gegen unbefugte oder unabsichtliche Benutzung gesichert werden. Kontaktieren Sie anschließend den Technischen Service von QIAGEN. Die elektrische Sicherheit des Geräts ist nicht mehr gegeben, wenn: Das Netzkabel beschädigt ist. ...
  • Page 266 übernationaler, nationaler und regionaler Ebene eingehalten werden. OSHA: Occupational Safety and Health Administration (Vereinigte Staaten von Amerika) † ACGIH: American Conference of Government Industrial Hygienists (Vereinigte Staaten von Amerika) ‡ COSHH: Control of Substances Hazardous to Health (Vereinigtes Königreich) QIAcube HT User Manual 07/2013...

  • Page 267: Chemikalien

    Substanzen müssen unter einem funktionierenden Laborabzugssystem durchgeführt werden, damit die möglicherweise entstehenden Dämpfe abziehen können. Entsorgen von Abfällen Benutzte Kunststoff-Laborartikel können gefährliche Chemikalien enthalten. Derartige Abfälle müssen gemäß den geltenden regionalen Sicherheitsbestimmungen gesammelt und entsorgt werden. QIAcube HT User Manual 07/2013...

  • Page 268: Gefahren Durch Mechanische Teile

    Appendix D Beachten Sie bei der Entsorgung des QIAcube HT die gesetzlichen Bestimmungen zur Entsorgung von Elektro- und Elektronik-Altgeräten (siehe Anhang A). Gefahren durch mechanische Teile WARNUNG Sich bewegende Geräteteile [W12] Vermeiden Sie jeglichen Kontakt mit sich bewegenden Geräteteilen, während der QIAcube HT in Betrieb ist. Auf gar keinen Fall dürfen sich Ihre Hände unter dem...

  • Page 269: Sicherheitshinweise - Wartungsarbeiten

    Stellen Sie keine Gegenstände auf den Roboterarm.  Wählen Sie die Option “Pause” in der Werkzeugleiste  der QIAcube HT Software, um das Gerät anzuhalten, bevor Sie Reaktionsgefäße oder Platten von der Arbeitsplattform entfernen. Warten Sie, bis der Roboterarm die gerade ablaufende Bewegung beendet hat –...
  • Page 270 Appendix D ACHTUNG Beschädigung der QIAcube HT Gerätehaube [C5] Verwenden Sie weder Alkohol noch Lösungsmittel oder Reagenzien, die Säuren, Laugen oder Abrasivstoffe enthalten, um die Gerätehaube zu reinigen. Alkohol schädigt die Gerätehaube. Verwenden Sie zum Reinigen der Gerätehaube ausschließlich destilliertes Wasser.

  • Page 271: Symbole Auf Dem Qiacube Ht

    Appendix D Symbole auf dem QIAcube HT Symbol Beschreibung Arbeitsplattform und Symbol für Warnhinweise Geräterückseite („Warnung“ oder „Achtung“) Arbeitsplattform und am Warnung for Biogefährdung Ausgang der Pipettenspitzen-Rutsche Arbeitsplattform und Warnung vor Handverletzung Roboter Arm Typenschild auf der CE-Kennzeichen (Zertifizierung Geräterückseite gemäß...
  • Page 272 Appendix D Symbol Beschreibung Typenschild auf der Markierung gemäß RoHS-Richtlinie Geräterückseite für China (Beschränkung der Verwendung bestimmter gefähr- licher Stoffe in Elektro- und Elektronikgeräten) Arbeitsplattform Lesen Sie die Gebrauchshinweise im Handbuch D-12 QIAcube HT User Manual 07/2013...

  • Page 273: Qiacube Ht And Qiaxtractor Accessories

    Plasticware and accessories Accessories Upgrade kit for QIAxtractor instrument; 9022649 Pack, QXT Adapter set to use dedicated QIAcube HT kits on the QIAxtractor Contains: Transfer Carriage (9022654), Riser Block EMTR (9022655), and Channeling Adapter (9022656) Gasket for...
  • Page 274 990550 tip ejector chute For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.

  • Page 275: Advanced Settings

    Appendix F Advanced settings Advanced run settings are available to adjust specific functions on the QIAcube HT. These functions should be modified only under direct instruction from QIAGEN. Tip pickup mode This defines the strategy used for collecting tips from the Tip Rack Holder.
  • Page 276 The recommended range is 0 to 3 mm. The default value is 3. Note: Losses for reagent liquids are typically greater than those for non-reagents. QIAcube HT User Manual 07/2013...
  • Page 277 “Reaction” right-hand pane, work only with volumes up to the largest capacity tip supported on the QIAcube HT (i.e., 200 µl). Activating this option allows those functions to work with volumes larger than 200 µl. This function is activated by default.
  • Page 278 In short, for each reaction plate the software determines the highest index reaction well required by a “Preserve source well indices” liquid handling operation and treats the next well as the first available for all other QIAcube HT User Manual 07/2013...
  • Page 279 This reduces the likelihood of droplet formation on the outer walls of the tips during transport and helps to manage drips. QIAcube HT User Manual 07/2013...
  • Page 280 The slower, smoother movement reduces the likelihood of drips from the tips. Although there is no QIAcube HT User Manual 07/2013...
  • Page 281 The recommended range of values is 10 to 100 (%). For viscous liquids a value in the range 30 to 60 is suggested. The default value is 100. QIAcube HT User Manual 07/2013...
  • Page 282 The transfer usually uses default pipetting speed, air intake, and blowout. Select this function to set the pipetting speed for lysates in a range from 60 to 450 µl/sec. In addition, the air intake after a lysate ejection can be QIAcube HT User Manual 07/2013...
  • Page 283 The slower, smoother movement reduces the likelihood of drips from the tips and avoids risks of cross contamination. This function is activated by default. QIAcube HT User Manual 07/2013...
  • Page 284 This 2-step operation is more robust than using a single movement to raise the carriage to the transfer height. This function is activated by default. F-10 QIAcube HT User Manual 07/2013...

  • Page 285: Sample And Reaction Functions For Non-Wizard Protocols

    The default is “No accessory”. 5. When the correct accessory has been selected, click “OK” to apply the selection and close the window. When an accessory is chosen, the background color of the position changes in the workspace. QIAcube HT User Manual 07/2013...
  • Page 286 Specify the default tip rack configuration as follows: 1. Open the software workspace (see Section 5.3, page 5-6). 2. Right-click the plate that is to be changed. 3. From the menu that appears, select “Change plate type“. The “Plate Configuration” window appears. QIAcube HT User Manual 07/2013...
  • Page 287 “Plate” list. Only the options that are appropriate for the selected position are displayed. 6. From the “Plate” list, select “96 QIAGEN OnCor C 200 µl Tips”. 7. Select the following “Plate Usage Options”: Pipet Order: “Vertical”...
  • Page 288 Add list of wells Opens the “Update Sample Bank” window on plate to and enables a list of wells to be defined sample bank and added to a new or existing sample bank. QIAcube HT User Manual 07/2013...
  • Page 289 Enables the user to enter the volume of volume liquids which have been preloaded into reaction tubes. This option is only available when a reaction plate is selected. Click “OK” to continue. “Update sample bank” window. QIAcube HT User Manual 07/2013...
  • Page 290 “INSIDE” or “OUTSIDE” the defined concentration range. Apply filter to Add or remove samples from a sample selected wells bank, based on a defined concentration range. The filter is immediately applied to the current selection. QIAcube HT User Manual 07/2013...
  • Page 291 “Modifying an existing sample bank” (below). Modifying an existing sample bank 1. To select all of the wells within a plate, click a sample or reagent plate. To select a group of wells within a plate, QIAcube HT User Manual 07/2013...
  • Page 292 These facilities extend the use of QIAcube HT beyond its primary purpose as a wizard-driven nucleic acid purification system. Two buttons on the reaction right-hand pane are used to update the reaction list: Add —...
  • Page 293 3. Click “Add...” in right-hand pane. The “Reaction Configuration” window appears. When the option “Allow multiple reagent volumes” is activated in the “Run Settings” menu (see page F-3) the “Reaction Configuration” dialog box is displayed with the advanced reagents view: QIAcube HT User Manual 07/2013...
  • Page 294 “Target reaction plate” drop-down list. To display all available reaction plates in a new window, click the button. 7. Enter the total volume of the final mixture in the “Made up to this volume:” field. G-10 QIAcube HT User Manual 07/2013...
  • Page 295 4. Select the desired operation (see the following sections for descriptions). 5. Set any required parameters in the operation configuration window that appears, and click “OK” to save the newly set up operation or “Cancel” to discard the operation. QIAcube HT User Manual 07/2013 G-11...
  • Page 296 A1. No additional configuration is needed. Special operation: Add “pause until confirmation” This function displays an optional user-defined message and pauses the instrument until a specified user action is performed and “OK” is clicked. G-12 QIAcube HT User Manual 07/2013...
  • Page 297 The “Wait for confirmation before proceeding?” option pauses the instrument until the user clicks “OK”. Alternatively, the fixed duration of vacuum application can be specified. QIAcube HT User Manual 07/2013 G-13...
  • Page 298 This option allows the user to control the position of the pinch valve for application of vacuum to the specified chambers. Special operation: Add “fan on/off” This function instructs the instrument to turn the HEPA filter on or off during a run. G-14 QIAcube HT User Manual 07/2013...
  • Page 299 8 samples is replicated 5 times using standard “general liquid handling” reaction steps (i.e., prepared using “Add…”), 40 tips are required. When the “Add sample bank duplication step…” option is used, only 8 tips are required for this operation. QIAcube HT User Manual 07/2013 G-15...
  • Page 300 Use new tips Instructs the instrument to use a new tip to transfer each replicate. To configure multi-eject options, click the “Multi-Eject Settings”. G-16 QIAcube HT User Manual 07/2013...
  • Page 301 Choose a starting well and define the transfer volume for a channel. First well Defines the first well of the reaction plate into which the sample bank will be replicated. Volume Defines the sample volume that will be used for replication. QIAcube HT User Manual 07/2013 G-17...
  • Page 302 Special operation: Add “discard tips” For runs configured without the “Vacuum extraction” wizard, this function allows used tips remaining on the worktable to be discarded explicitly. This function is typically used at the end of a run. G-18 QIAcube HT User Manual 07/2013...

  • Page 303: Description Of The Generic Xml Format

    <Wells> <Well> <Label>A1</Label> <WellID/> <WellNo>1</WellNo> <StandardWellName>A:1</StandardWellName> <Color>5775359</Color> <TubeType>BioSprint_250</TubeType> <Concentration>37</Concentration> <FinalWellVol>80</FinalWellVol> <EventOccurred>0</EventOccurred> <Contents> <Diluent> <SocketName>M1</SocketName> <WellLabel>A</WellLabel> <Vol>40</Vol> </Diluent> <Mix> <SocketName>M1</SocketName> <WellLabel>B</WellLabel> <Vol>10</Vol> <Name>Mix B</Name> </Mix> <Reagent> <SocketName>R1</SocketName> <WellLabel>I</WellLabel> <Vol>2.5</Vol> <Name>10X Buffer</Name> </Reagent> <Sample> <SocketName>C1</SocketName> <PlateID/> <WellLabel>A1</WellLabel> QIAcube HT User Manual 07/2013...
  • Page 304 To make the user’s selection for empty wells and sample labels evaluable, two parameters are passed to the XSL transformation: • SampleLabelType Possible values: SL_FullID = 0 SL_SampleID = 1 SL_ReactionID = 2 SL_SampleName = 3 QIAcube HT User Manual 07/2013...
  • Page 305 Appendix H • EmptyLabelType Possible values: EL_Empty = 0 EL_WellID = 1 EL_WellOrder = 2 EL_WellNumber = 3 They can be evaluated during the transformation. QIAcube HT User Manual 07/2013...
  • Page 306 Appendix H This page intentionally left blank QIAcube HT User Manual 07/2013...
  • Page 307 5-72 assembling, 6-3, 6-7 calibrating position, 5-69 Elution riser block favorite, 5-27 assembling, 6-7 function, 5-28 use, 3-11 plate buttons, 5-9 File location, 5-119 properties, 5-24 Firmware version number, 5-17 type, 5-26 QIAcube HT User Manual 07/2013 Index-1...
  • Page 308 Run file use, 6-8 use, 5-89 QIAcube HT Run file:, 5-94 intended use, 2-2 Run modes, 5-2 QIAcube HT kits, 2-4, 3-7 Run settings software, 2-4 advanced, 1 vacuum chamber, 3-7 reset default, 5-120 QIAcube HT instrument save as new default, 5-121...
  • Page 309 Waste sink/mat support other QIAGEN plates, 3-9 assembling, 6-5 Troubleshooting, 8-1 use, 3-10 important error messages, 8-1 Workspace run setup errors, 8-1 items, 5-23 USB interface, 4-16 use, 5-6 User management, 5-61 Worktable, 3-6 UV lamp QIAcube HT User Manual 07/2013 Index-3...
  • Page 311 Australia techservice-au@qiagen.com  Austria techservice-at@qiagen.com  Belgium techservice-bnl@qiagen.com  Brazil suportetecnico.brasil@qiagen.com  Canada techservice-ca@qiagen.com  China techservice-cn@qiagen.com  Denmark techservice-nordic@qiagen.com  Finland techservice-nordic@qiagen.com  France techservice-fr@qiagen.com  Germany techservice-de@qiagen.com  Hong Kong techservice-hk@qiagen.com  India techservice-india@qiagen.com  Ireland techservice-uk@qiagen.com ...

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