Qiagen QIAcuity 911000 User Manual page 124

Reasons for the invalidation of an image are:
Too less fluorescence signal, e.g. when the Nanoplate was re-imaged after a long storage

period (see Operating plates for information about the storage of plates)
Vibration during the imaging process can lead to blurry images. If the image of the reference

channel is affected, the number of valid partitions cannot be determined and the whole well is
invalidated for analysis. If a target channel is affected, only the image of the respective
channel is invalidated for analysis.
Incomplete filling of a well can lead to too less valid partitions in the reference channel

needed for analysis. In this case, the whole well is invalidated.
Image corrective measures
To ensure proper analysis based on valid partitions, artefacts that could influence the result analysis
are removed from the images. The corrections are done automatically by the QIAcuity Software
Suite and don't require any user action. The partitions that are affected by artefacts are blacked
out and are invalidated for further analysis. Artefacts can be:
Dust and other particles

Low amplification areas

Areas of bad filling

QIAcuity User Manual 07/2020 124