Qiagen EZ1 Advanced User Manual
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Qiagen EZ1 Advanced User Manual

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EZ1
®
Advanced User Manual
9001410, 9001411
1050261
QIAGEN GmbH, D-40724 Hilden
R2
Sample & Assay Technologies
May 2008

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  • Page 1 May 2008 ® Advanced User Manual 9001410, 9001411 1050261 QIAGEN GmbH, D-40724 Hilden Sample & Assay Technologies...
  • Page 2 , EZ1 ® (QIAGEN Group); DECON-QUAT ® (Veltek Associates, Inc.); Gigasept ® , Lysetol ® , Mikrozid ® (Schülke & Mayr GmbH); ® (International Business Machines Corporation); Microsoft ® , Windows ® (Microsoft Corporation). © 2008 QIAGEN, all rights reserved.

  • Page 3: Table Of Contents

    About this user manual General information 2.2.1 Technical assistance 2.2.2 Policy statement 2.2.3 Version management Intended use of the EZ1 Advanced 2.3.1 Requirements for EZ1 Advanced users General Description External features of the EZ1 Advanced 3.1.1 EZ1 Advanced door 3.1.2 Control panel 3.1.3...
  • Page 4 AC power connection Hardware installation 4.3.1 Unpacking the EZ1 Advanced 4.3.2 Installing the EZ1 Advanced 4.3.3 Configuring and setting up the EZ1 Advanced 4.3.4 Installing the printer (optional) 4-10 4.3.5 Installing the bar code reader 4-10 Installing the PC software 4-11 4.4.1...
  • Page 5 Contents 5.4.1 Opening the EZ1 Advanced door 5.4.2 Closing the EZ1 Advanced door Starting and stopping a protocol run 5.5.1 Starting and finishing a protocol run 5.5.2 Stopping a protocol run Setting up the worktable 5.6.1 Removing and replacing the tray 5.6.2...
  • Page 6 Contents 5.13 Generating a report file 5-32 5.14 Using the EZ1 Advanced Communicator software 5-36 5.14.1 User interface 5-36 5.15 Using multiple EZ1 Advanced instruments 5-40 5.16 Evaluating pipetting accuracy 5-40 Preventive Maintenance Regular maintenance procedure Daily maintenance procedure Weekly maintenance procedure...

  • Page 7: Safety Information

    The advice given in this manual is intended to supplement, not supersede, the normal safety requirements prevailing in the user’s country. EZ1 Advanced User Manual 05/2008...

  • Page 8: Proper Use

    Risk of personal injury [W2] Two people must carry the instrument. Use the handle attached to the box to lift the EZ1 Advanced. After the EZ1 Advanced has been unpacked, 2 people must lift the instrument. Lift the instrument by placing your hands underneath the bottom of the instrument.

  • Page 9: Electrical Safety

    CAUTION Damage to the instrument [C2] Ensure that the EZ1 Advanced is switched off before you manually move the mechanical components of the instrument. Electrical safety To ensure satisfactory and safe operation of the EZ1 Advanced, it is essential that the line power cord is connected to true electrical earth (ground).

  • Page 10: Environment

    Environment Operating conditions WARNING Explosive atmosphere [W6] The EZ1 Advanced is not designed for use in an explosive atmosphere. Biological safety Specimens and reagents containing materials from humans should be treated as potentially infectious. Use safe laboratory procedures as outlined in publications such as Biosafety in Microbiological and Biomedical Laboratories, HHS (www.cdc.gov/od/ohs/biosfty/biosfty.htm).
  • Page 11 OSHA: Occupational Safety and Health Administration (United States of America). ACGIH: American Conference of Government Industrial Hygienists (United States of America). COSHH: Control of Substances Hazardous to Health (United Kingdom). EZ1 Advanced User Manual 05/2008...

  • Page 12: Chemicals

    Such wastes must be collected and disposed of properly in accordance with the local safety regulations. For disposal of waste electrical and electronic equipment (WEEE), see page A-6. EZ1 Advanced User Manual 05/2008...

  • Page 13: Mechanical Hazards

    Safety Information Mechanical hazards The worktable of the EZ1 Advanced moves during operation of the instrument. WARNING Moving parts [W9] Never open the EZ1 Advanced door while the EZ1 Advanced is operating. WARNING Risk of overheating [W10] To ensure proper ventilation, maintain a minimum clearance of 15 cm and 30 cm at the rear and sides of the EZ1 Advanced, respectively.

  • Page 14: Symbols On The Ez1 Advanced

    Safety Information 1.10 Symbols on the EZ1 Advanced Symbol Location Description Heating Heat hazard — the temperature of the system heating system can reach up to 95ºC Near the Biohazard — the tip rack may be tip rack contaminated with biohazardous material...

  • Page 15: Additional Symbols In This User Manual

    Serial number on the back of the instrument Type plate Legal manufacturer on the back of the instrument 1.11 Additional symbols in this user manual Symbol Location Description Front Catalog number cover Front Handbook cover EZ1 Advanced User Manual 05/2008...
  • Page 16 Safety Information Symbol on the heating system. Symbol near the tip rack. Symbol on back of the EZ1 Advanced. 1-10 EZ1 Advanced User Manual 05/2008...

  • Page 17: Introduction

    Introduction Introduction Thank you for choosing the QIAGEN EZ1 Advanced. We are confident it will become an integral part of your laboratory. Before using the instrument, it is essential to read this manual carefully and to pay particular attention to any advice it contains concerning hazards that may arise from use of this instrument.

  • Page 18: Policy Statement

    (see back cover or visit www.qiagen.com ). 2.2.2 Policy statement It is the policy of QIAGEN to improve products as new techniques and components become available. QIAGEN reserves the right to change specifications at any time. In an effort to produce useful and appropriate documentation, we appreciate your comments on this publication.

  • Page 19: Requirements For Ez1 Advanced Users

    Introduction 2.3.1 Requirements for EZ1 Advanced users This table covers the general level of competence and training necessary for transportation, installation, use, maintenance, and servicing of the EZ1 Advanced. Type of task Personnel Training and experience Delivery No special No special requirements...
  • Page 20 Introduction This page intentionally left blank EZ1 Advanced User Manual 05/2008...

  • Page 21: General Description

    The user then closes the EZ1Advanced door and starts the protocol. The door locks automatically at the start of the protocol. The protocol provides the necessary instructions for the EZ1 Advanced to carry out automated nucleic acid purification. The aspiration and dispensing of samples and reagents and the separation of magnetic particles are performed by the 6-channel pipettor head.

  • Page 22: External Features Of The Ez1 Advanced

    Status LEDs 3.1.1 EZ1 Advanced door The EZ1 Advanced door can be manually opened by the user in order to gain access to the worktable when no protocol is running. A protocol cannot be started until the EZ1 Advanced door is closed.
  • Page 23 SHIFT + Used to enter the installation date. Up/Dn The other keys of the control panel are for service personnel only. Vacuum fluorescent display (VFD) The display consists of 4 lines with 20 characters per line. EZ1 Advanced User Manual 05/2008...

  • Page 24: Ez1 Advanced Card Slot

    Advanced. Other EZ1 Cards cannot be used with the EZ1 Advanced. Note: Do not insert the EZ1 Advanced Card while the EZ1 Advanced is switched on. EZ1 Advanced Cards must not be exchanged while the EZ1 Advanced is switched on. Take care not to expose the EZ1 Advanced Card to electric shock, water, or dirt.

  • Page 25: Status Leds

    General Description EZ1 Advanced Card inserted into EZ1 Advanced Card slot. 3.1.4 Status LEDs There are 2 light-emitting diodes (LEDs) at the front of the EZ1 Advanced: Green LED — indicates that the instrument is receiving power Red LED — indicates that an error has occurred 3.1.5...

  • Page 26: Internal Features Of The Ez1 Advanced

    RS232 connectors. The left connector is used to connect the bar code reader. The right connector is used to connect the EZ1 Advanced to a PC or an RS232 printer (optional). Internal features of the EZ1 Advanced The interior of the EZ1 Advanced contains the worktable and the pipettor head.
  • Page 27 (Note: Some protocols require only one row of filter- tips.) The remaining row holds up to 6 sample tubes. Sample tubes are 2 ml in volume, screw-capped, made of polypropylene, supplied by Sarstedt (cat. no. 72.693), and provided in EZ1 Kits. EZ1 Advanced User Manual 05/2008...
  • Page 28 General Description Tip rack. Both tip holders and filter-tips are made of polypropylene and are provided in EZ1 Kits. The filter-tips have a capacity of 50–1000 μl. EZ1 Advanced User Manual 05/2008...
  • Page 29 This rack is located behind the tip rack and holds up to 6 reagent cartridges. Insert the cartridge rack with the arrow pointing towards the instrument. Cartridge rack. Reagent cartridges are made of polypropylene, contain prefilled reagents, and are available from QIAGEN. EZ1 Advanced User Manual 05/2008...
  • Page 30 The heating system is located under the far end of the cartridge rack. It holds the heating positions of the reagent cartridges. Tray The tray is located under the racks and collects any drops of liquid that may fall. Tray. 3-10 EZ1 Advanced User Manual 05/2008...

  • Page 31: Pipettor Head

    6 metal spikes for puncturing the foil that covers the reagent cartridges. During operation, the piercing unit, automatically controlled by the EZ1 Advanced, first exposes the reagents in the reagent cartridges. The pipettor head then automatically picks up filter-tips from the tip rack and performs aspiration and dispensing operations at different locations on the worktable before ejecting the tips back into the tip rack.

  • Page 32: Uv Lamp

    3.2.3 UV lamp The EZ1 Advanced is equipped with a UV lamp, located under the blue EZ1 Advanced door. The UV lamp can be manually switched on and off in a separate menu, as described later in this manual. Do not touch the UV lamp with your fingers since it may reduce the lamp’s lifetime.
  • Page 33 General Description UV lamp Location of UV lamp under the EZ1 Advanced door. CAUTION Cover of UV lamp [C5] Be careful not to damage the cover of the UV lamp when loading and unloading the worktable. EZ1 Advanced User Manual 05/2008...
  • Page 34 General Description This page intentionally left blank 3-14 EZ1 Advanced User Manual 05/2008...

  • Page 35: Installation Procedures

    Requirements Site The EZ1 Advanced must be located out of direct sunlight, away from heat sources, and away from sources of vibration and electrical interference. Refer to Appendix A for the operating conditions (temperature and humidity).
  • Page 36 Installation of AC power cord Connect one end of the AC power cord to the socket located on the rear of the EZ1 Advanced and the other end to the AC power outlet. The fuse box of the EZ1 Advanced is located under the socket...

  • Page 37: Hardware Installation

    100–120 V line power supply. WARNING Electrical hazard [W13] Never install a fuse different from that specified in the user manual. Hardware installation The following items are delivered: EZ1 Advanced International power cord set (5 countries) EZ1 Advanced User Manual 05/2008...

  • Page 38: Unpacking The Ez1 Advanced

    Fuses (1 each: 6.3 A and 3.15 A) Bar code reader EZ1 Advanced User Manual EZ1 Advanced teaching data sheet CD with EZ1 Advanced Communicator software 4.3.1 Unpacking the EZ1 Advanced 1. Cut and remove the plastic straps. Plastic straps...
  • Page 39 4. Remove the cardboard tray. 5. Pull out the 4 corner shock absorbers at the top, and remove the outer cardboard box by pulling it up. 6. Take out the wrapped EZ1 Advanced, and remove the aluminum strap. EZ1 Advanced User Manual 05/2008...
  • Page 40 EZ1 Advanced. Removing the protectors for the Y- and Z-axes During transportation, protectors prevent the moveable parts of the EZ1 Advanced from moving along the Y- and Z-axes. Before using the instrument, these protectors must be removed.
  • Page 41 Installation Procedures Cable ties Bubble wrap Silica gel 4. Push the pipettor head upward. Pipettor head EZ1 Advanced User Manual 05/2008...

  • Page 42: Installing The Ez1 Advanced

    3. Connect the handheld bar code reader to the EZ1 Advanced (Section 4.3.5, page 4-10). 4. Connect the PC to the EZ1 Advanced. Use the RS232 cable and plug it into the R232 connector labeled “PC/Printer” at the rear of the instrument (Section 3.1.5, page 3-5).

  • Page 43: Configuring And Setting Up The Ez1 Advanced

    Configuring and setting up the EZ1 Advanced Setting the installation date The first time the EZ1 Advanced is switched on, it is necessary to enter the installation date. This date will be recorded in the report file described below. The installation date is also used to let the instrument know when to show the weekly and annual maintenance reminders.

  • Page 44: Installing The Printer (Optional)

    25-pin connector on the other end. Connect the printer’s data cable to the EZ1 Advanced. The connector is located at the rear of the instrument, labeled “PC/Printer” (Section 3.1.5, page 3-5). Make sure that the connector is secured with the 2 screws provided.

  • Page 45: Installing The Pc Software

    Installation Procedures Connect the bar code data cable to the EZ1 Advanced. The connector is located at the rear of the instrument, labeled “Barcode Reader” (Section 3.1.5, page 3-5). Make sure that the connector is secured with the 2 screws provided. Switch on the EZ1 Advanced.

  • Page 46: System Requirements

    System requirements ® compatible PC (e.g., from QIAGEN, cat. no. 9016319, and monitor, cat. no. 9016308) Note: To operate multiple (up to 4) EZ1 Advanced instruments with a single PC, only the QIAGEN PC (cat. no. 9016319) can be used. ®...
  • Page 47 Installation Procedures Mapping the COM port Click the “Start” button on your PC and select “Settings/Control Panel”. Double-click “System”. Click the “ Hardware” tab and select the “Device Manager” button. EZ1 Advanced User Manual 05/2008 4-13...
  • Page 48 Expand “Ports” to show all available COM ports. Connect the converter to an open USB port on the PC. The device manager should then automatically update the COM port list. Look for the COM port named “USB to Serial Bridge”. 4-14 EZ1 Advanced User Manual 05/2008...
  • Page 49 Click the “Port Setting” tab and select the “Advanced” button. Change the dialog field “COM Port Number” to “COM1”. The USB port is now mapped to COM port 1. Always use COM Port 1 to work with the EZ1 Advanced. EZ1 Advanced User Manual 05/2008 4-15...

  • Page 50: Installing The Ez1 Advanced Communicator Software

    The EZ1 Advanced Communicator software is now ready to receive data from the EZ1 Advanced. In order to check the connectivity between the PC and EZ1 Advanced, use the test described in Section 5.9.3. 4-16 EZ1 Advanced User Manual 05/2008...

  • Page 51: Installing Multiple Ez1 Advanced Instruments

    Installation Procedures Installing multiple EZ1 Advanced instruments Up to 4 EZ1 Advanced instruments can be connected to a single PC. For this application, only the QIAGEN PC (cat. no. 9016643) can be used. This PC is equipped with a 1 x 4 Port Serial board. To set up your PC, follow the directions given below.
  • Page 52 EZ1 Advanced. See Section 3.1.5 for more information. Use the connector for PC/printer. If you use multiple EZ1 Advanced instruments, you do not need to install the driver for the USB-RS232 converter. Click the “Start” button on your PC and select “Settings/Control Panel”.

  • Page 53: Transportation And Removal Of The Ez1 Advanced

    EZ1 Advanced, use the test described in Section 5.9.3. Transportation and removal of the EZ1 Advanced If the EZ1 Advanced needs to be transported, please follow your organization’s instructions and guidelines. In addition, follow the steps below. EZ1 Advanced User Manual 05/2008...
  • Page 54 Installation Procedures 1. Decontaminate the instrument. 2. Pack the EZ1 Advanced by following the steps of the unpacking procedure described in Section 4.3.1 in reverse order. Use original packaging material. 3. Appendix A gives information about transportation conditions. If the EZ1 Advanced needs to be sent to QIAGEN, please contact your local instrument service organization.

  • Page 55: General Operation

    For details, refer to the handbook supplied with the EZ1 Kit you are using. 4. If you want EZ1 Advanced to generate a report file, you will be asked to enter additional data. 5. Open the EZ1 Advanced door, and set up the worktable according to the messages shown in the display.

  • Page 56: Inserting The Ez1 Advanced Card

    Advanced Card CAUTION Damage to the instrument [C6] The EZ1 Advanced Card is an integrated part of the system. Ensure that the EZ1 Advanced is always switched off before you insert or remove the EZ1 Advanced Card. 5.2.1 Inserting the EZ1 Advanced Card 1.
  • Page 57 General Operation Orient the card so that the picture faces to the left and the triangle symbol points towards the EZ1 Advanced Card slot. Ensure that the card is completely inserted into the slot. EZ1 Advanced User Manual 05/2008...

  • Page 58: Removing The Ez1 Advanced Card

    Removing the EZ1 Advanced Card 1. Switch off the EZ1 Advanced (Section 5.3.2, page5-5). 2. Turn the cover of the EZ1 Advanced Card slot. 3. Press the button at the bottom of the EZ1 Advanced Card slot. The EZ1 Advanced Card will be ejected.

  • Page 59: Switching The Ez1 Advanced On And Off

    5.3.1 Switching on the EZ1 Advanced 1. Insert the EZ1 Advanced Card (Section 5.2.1, page 5-2). 2. Switch on the EZ1 Advanced at the rear power switch. 3. The display shows the following screen for a few seconds. This text indicates that the EZ1 Advanced is initializing.

  • Page 60: Closing The Ez1 Advanced Door

    General Operation 5.4.2 Closing the EZ1 Advanced door 1. Press the door down to disengage the door lock. 2. Gradually lower the door until it rests on the worktable. Take care that your fingers do not get caught between the door and worktable.

  • Page 61: Starting And Stopping A Protocol Run

    EZ1 Kit you are using. 3. If you want the EZ1 Advanced to generate a report file, you will be asked to enter additional data, such as user ID, bar code of the kit, and bar code of the samples.

  • Page 62: Stopping A Protocol Run

    2. Press “STOP” again to abort the protocol. The protocol run will be invalid and will be marked accordingly in the report file. The following screen appears. 01 Apr 2008 10:30 START:Run 1:UV 2:Man 3:Test 4:Setup Key:START,1,2,3,4 EZ1 Advanced User Manual 05/2008...

  • Page 63: Setting Up The Worktable

    Removing and replacing the tray The tray is located under the worktable and can be removed for cleaning. 1. Switch off the EZ1 Advanced. 2. Push the worktable toward the back of the EZ1 Advanced. 3. Remove the tray using its handle.

  • Page 64: Loading The Reagent Cartridges

    3. Remove the cartridge rack from the worktable. 4. Slide the reagent cartridges into the cartridge rack in the direction of the arrow, as shown below, until you feel resistance. Press down the cartridges until they click into place. 5-10 EZ1 Advanced User Manual 05/2008...

  • Page 65: Loading The Elution Tubes, Filter-Tips, And Sample Tubes

    EZ1 Kit handbook. Label the elution tubes before you load them into the rack. Make sure that the caps of the tubes are removed before you start the protocol. EZ1 Advanced User Manual 05/2008 5-11...
  • Page 66 General Operation Make sure that the caps of the sample tubes are removed before you start the protocol. 4. Return the tip rack to the worktable. 5-12 EZ1 Advanced User Manual 05/2008...

  • Page 67: External Tube Rack

    UV lamp on as described below. Note: UV decontamination helps to reduce possible pathogen contamination of the EZ1 Advanced worktable surfaces. The efficiency of inactivation has to be determined for each specific organism and depends, for example, on layer thickness and sample type.

  • Page 68: Switching On The Uv Lamp

    General Operation 5.7.1 Switching on the UV lamp 1. Make sure that the EZ1 Advanced door is closed. In the main menu, press “1” to select the UV light function. 01 Apr 2008 10:30 START:Run 1:UV 2:Man 3:Test 4:Setup Key:START,1,2,3,4 The following screen appears.

  • Page 69: Switching Off The Uv Lamp

    Decontamination>Run UV lamp cooling Please stand by For user safety, the UV lamp cools for approximately 3 minutes. The EZ1 Advanced door cannot be opened until after the cooling time has elapsed. After cooling, the main menu appears. 5.7.2 Switching off the UV lamp...

  • Page 70: Lifetime Reminder Of The Uv Lamp

    Contact QIAGEN Technical Services for replacement of the UV lamp. 5.7.4 UV lamp ignition error If the UV lamp fails to ignite, the EZ1 Advanced will retry 2 more times. If the retries do not ignite the lamp, the following error message appears. ERROR:UV Lamp...

  • Page 71: Manual Operation

    Y: The worktable moves to its park position. Z: The nozzle unit will move to its park position. P: The piercing unit will move to its park position. M: The magnet unit will move to its park position. EZ1 Advanced User Manual 05/2008 5-17...

  • Page 72: Manual Operation "Return Tip

    Key:START,ESC Press “START” to start the “return tip” operation or “ESC” to return to the previous screen. 5.8.3 Manual operation “clean” Use the “clean” operation to get access to the piercing unit for cleaning. 5-18 EZ1 Advanced User Manual 05/2008...

  • Page 73: Manual Operation "Resend

    General Operation The EZ1 Advanced door must initially be closed. Press ”3” in the manual operation screen to choose the “clean” operation. The following screen appears. MANUAL OPERATION clean piercing unit START:Run Key:START,ESC Press “START” to start the “clean” operation or “ESC” to return to the previous screen.

  • Page 74: Test

    Press “ESC”. Check the connection to the PC or printer. Make sure that the PC or printer is turned on. If using a PC, make sure that the EZ1 Advanced Communicator software is installed and running on the PC. If the transmission problem cannot be fixed, contact QIAGEN Technical Services.

  • Page 75: Test Axis

    Move the magnet unit to “narrow” position and then return to home. Aspirate and dispense to test the pipetting unit. Move to Proc 2–Proc 11 positions (see figure on next page). Move to elution position. Release tip 1. EZ1 Advanced User Manual 05/2008 5-21...

  • Page 76: Test Heating Block

    Eluate Proc positions tested by the EZ1 Advanced test procedure. 5.9.2 Test heating block This function tests if the heating block of the EZ1 Advanced is operating. Press ”2” in the test screen to choose the “Temp” operation. 5-22 EZ1 Advanced User Manual 05/2008...

  • Page 77: Test Serial Port

    “X” if the temperature is outside the given range. 5.9.3 Test serial port Press ”3” in the test screen to choose the “Serial” operation. TEST 1:Axis 2:Temp 3:Serial 4:Version Key:1,2,3,4,ESC EZ1 Advanced User Manual 05/2008 5-23...
  • Page 78 If the transmission is successful, the result will show “PASSED”; if not successful, it will show “FAILED”. Printer Press “START” to send a test string to the printer. The following screen appears after transmission is complete. 5-24 EZ1 Advanced User Manual 05/2008...
  • Page 79 Q-Card included in an EZ1 Kit). When a new bar code is read, the previous bar code will be overwritten. A beep sounds after a successful scan of the bar code. The following screen appears, showing up to 25 digits. EZ1 Advanced User Manual 05/2008 5-25...

  • Page 80: Test Version

    Press “ESC” to return to the test screen. 5.10 System setup 5.10.1 Setting the date Press “4” in the main menu to select system setup. 01 Apr 2008 10:30 START:Run 1:UV 2:Man 3:Test 4:Setup Key:START,1,2,3,4 5-26 EZ1 Advanced User Manual 05/2008...

  • Page 81: Setting The Time

    Alternatively, press “ESC” to leave the date unchanged. 5.10.2 Setting the time Press ”2” in the system setup menu to change the time. SYSTEM SETUP 1:Date 2:Time 3:Serial Port 4:PM Key:1,2,3,4,ESC The following screen appears. EZ1 Advanced User Manual 05/2008 5-27...

  • Page 82: Setting The Serial Port

    “CS” (current setting) indicates the current setting of the serial port as “PC”, “Printer”, or “Not Used”. Press the up arrow or the down arrow to change the “NS” (new setting) field to “PC”, “Printer”, or “Not Used”. 5-28 EZ1 Advanced User Manual 05/2008...

  • Page 83: Setting The Preventive Maintenance Reminder

    A red light appears. Hold the bar code reader in front of the bar code at a distance of approximately 20 mm. A beep will sound to EZ1 Advanced User Manual 05/2008 5-29...

  • Page 84: Using The External Printer (Optional)

    The bar code will be displayed on the screen. 5.12 Using the external printer (optional) The EZ1 Advanced allows you to confirm that a report was printed correctly before the report file is deleted. The following screen appears. SEND REPORT Print out o.k ?
  • Page 85 Note: If the printer is turned on before switching on the EZ1 Advanced, some additional characters will be printed before the report. To avoid this, switch on the EZ1 Advanced before switching on the printer. EZ1 Advanced User Manual 05/2008...

  • Page 86: Generating A Report File

    General Operation 5.13 Generating a report file The EZ1 Advanced will generate a report file if this option was selected during the protocol run. The report file contains the fields described on the next pages. 5-32 EZ1 Advanced User Manual 05/2008...
  • Page 87 Firmware version: V1.0.0 Current firmware version Installation date Apr 01, 2008 Installation date, set the first time of instr.: the EZ1 Advanced is powered Stored permanently on the EZ1 Advanced Weekly May 10, 2008 When you accept the weekly maintenance...
  • Page 88 Advanced Card and copied into report file Trace Additional protocol name (specifies the protocol if there are multiple protocols on the EZ1 Advanced Card) Date of run: May 14, 2008 Time stamp for date of run given through internal clock and...
  • Page 89 If the report file could not be transmitted (e.g., if the serial connection is broken), the report will remain stored on the EZ1 Advanced. After connection is reestablished, use the manual transmit function to resend the report file (see Section 5.8.4, page 5-19).

  • Page 90: Using The Ez1 Advanced Communicator Software

    5.14 Using the EZ1 Advanced Communicator software EZ1 Advanced Communicator is a software program that runs on a PC. The software receives the report file and stores it in a folder that you define. After the PC has received the...
  • Page 91 General Operation The report file name contains the following components. Title (e.g., EZ1_Report) Serial number of the EZ1 Advanced Date in format YYYY-MM-DD Time in format HH-MM-SS File extension *.csv (comma-separated value) The date and time are when the report file was received by the PC.
  • Page 92 General Operation COM1 should already be selected. You can check the serial settings by checking one of the boxes. The following window opens. 5-38 EZ1 Advanced User Manual 05/2008...
  • Page 93 Select “About” in the main window to show the version of the EZ1 Advanced Communicator software. Click the “Change path” button in the main window to change the folder where the selected report file is stored. The following window opens. Browse and select the folder. EZ1 Advanced User Manual 05/2008 5-39...

  • Page 94: Using Multiple Ez1 Advanced Instruments

    General Operation 5.15 Using multiple EZ1 Advanced instruments Up to 4 EZ1 Advanced instruments can be operated with a single PC. For this application, only the QIAGEN PC (cat. no. 9016643) can be used. The following combinations are available from QIAGEN.

  • Page 95: Preventive Maintenance

    Section 5.10.4, page 5-29) Maintenance reminders The EZ1 Advanced has a built-in clock and calendar device to remind you when the weekly or annual maintenance is due. The weekly maintenance reminder screen appears. Maintenance Reminder...
  • Page 96 If the weekly maintenance has not been completed, you can press “2” to indicate that the maintenance will be performed later. The next time the EZ1 Advanced is switched on, the reminder screen will appear again. The screen will continue to appear each time the EZ1 Advanced is switched on, until you press “1”...

  • Page 97: Regular Maintenance Procedure

    IQ/OQ, Warranty Extensions, Full Cover Support Agreements, and Preventive Maintenance Agreements. Service Support Agreements ensure high performance from your EZ1 Advanced. In addition, service histories are fully documented and all parts are certified and guaranteed. Contact your local QIAGEN Instrument Service representative, or your local distributor for more information about flexible Service Support Agreements from QIAGEN.
  • Page 98 MANUAL OPERATION Open door and clean piercing unit ENT:Done 6. Open the EZ1 Advanced door, and wipe the piercing unit using a soft tissue moistened with 70% ethanol. The piercing unit is sharp. Two pairs of gloves are recommended. EZ1 Advanced User Manual 05/2008...
  • Page 99 Piercing unit 7. Wipe the piercing unit using a soft tissue moistened with distilled water. 8. Close the EZ1 Advanced door and press “ENT”. The piercing unit returns to its original position. The manual operation screen appears. MANUAL OPERATION 1:Home 2:Return Tip...

  • Page 100: Daily Maintenance Procedure

    Preventive Maintenance Note: If you press “ENT” while the EZ1 Advanced door is open, an error message appears (see Section 7.1.1, page 7-1). 9. Press “ESC” to return to the main menu. 01 Apr 2008 10:30 START:Run 1:UV 2:Man 3:Test 4:Setup Key:START,1,2,3,4 10.

  • Page 101: Weekly Maintenance Procedure

    4. Clean the worktable and its racks with 70% ethanol, and then with distilled water. 5. Wipe the other surfaces of the EZ1 Advanced with diluted neutral soap solution, and then with water. 6. Wipe the O-rings of the tip adapters with a lint-free tissue.

  • Page 102: Reagents For Decontamination

    Note: The filter-tips should sit flush against the upper white plastic bar if the O-rings are properly greased. There should not be a gap. Excess or insufficient grease can affect the performance of the EZ1 Advanced. Reagents for decontamination The following disinfectants and detergents are compatible with metallic surfaces and removable components of the EZ1 Advanced system.
  • Page 103 100 (Veltek Associates, Inc.; www.sterile.com ).* Note: If you are not sure about the suitability of disinfectants or cleaning agents for use with the EZ1 Advanced, do not use them. Note: Do not spray cleaning or disinfectant liquids onto surfaces of the EZ1 Advanced. Spray bottles should be used only for items that have been removed from the EZ1 Advanced.
  • Page 104 Preventive Maintenance This page intentionally left blank 6-10 EZ1 Advanced User Manual 05/2008...

  • Page 105: Troubleshooting

    Troubleshooting Troubleshooting Detecting an error 7.1.1 EZ1 Advanced door errors If the EZ1 Advanced door is open when you start a protocol run, the following error screen will be displayed. ERROR:Door open! Close the door. Press ESC to retry. Key:ESC Close the door and press “ESC”...
  • Page 106 The second line indicates the line number of the protocol at which the error occurred. Record the error code and the line number, and contact QIAGEN Technical Services. Then reset the EZ1 Advanced by following the procedure given below. 1. Press “ESC” to display the main menu.

  • Page 107: Error Codes

    Motor for pipettor head movement (Z-axis) does not respond Motor for plunger/piercing unit movement (P-axis) does not respond Motor for magnet-unit movement (M-axis) does not respond Motor for worktable movement (Y-axis) does not respond during a protocol run EZ1 Advanced User Manual 05/2008...
  • Page 108 Door open when starting an operation Bottom sensor of pipettor head (Z-axis) activated during an operation Communication error between heating block and temperature controller, or card is not an EZ1 Advanced Card Communication error between motors and driver board – –...

  • Page 109: Glossary

    A card that contains one or more protocols for the EZ1 Card Advanced and is inserted into the instrument. EZ1 Advanced A slot at the front of the EZ1 Advanced that accepts an Card slot EZ1 Advanced Card. EZ1 Advanced...
  • Page 110 6 syringe pumps, each of which is connected to a tip adapter. Protocol A set of instructions for the EZ1 Advanced that allows the instrument to automate a nucleic acid purification procedure. Protocols are run using the control panel.
  • Page 111 Vacuum fluorescence display, a message-display screen using vacuum-tube technology. Worktable The surface of the EZ1 Advanced that contains racks and is where samples, reagent cartridges, and disposable labware are loaded. The worktable moves backwards and forwards to present different samples and reagents under the pipettor head.
  • Page 112 Glossary This page intentionally left blank EZ1 Advanced User Manual 05/2008...

  • Page 113: Technical Data

    Appendix A Appendix A Technical data QIAGEN reserves the right to change specifications at any time. Environmental conditions Operating conditions Power 100–120 V AC, 50/60 Hz, 240 VA (North America and Japan) 200–240 V AC, 50/60 Hz, 240 VA (Europe) Mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages.
  • Page 114 2K2 & 2M2 (IEC 60721-3-2) class Storage conditions Air temperature 5ºC to 40ºC (41ºF to 104ºF) if in manufacturer’s package Relative humidity Minimum 15% to maximum 75% (noncondensing) Environmental 1K2 & 1M2 (IEC 60721-3-1) class EZ1 Advanced User Manual 05/2008...
  • Page 115 Automated nucleic acid isolation using magnetic features particles Desktop instrument Protocols stored on EZ1 Advanced Cards To be used only with QIAGEN EZ1 Kits Aspirates and dispenses 6 samples or reagents simultaneously using a 6-channel pipettor head Separates magnetic particles using patented technology...
  • Page 116 Filter-tips Attach to the tip adapters of the pipettor head to allow liquid aspiration and dispensing. Capacity of 50–1000 μl. The EZ1 Advanced accommodates up to 12 tip holders, each containing a filter-tip, in the tip rack on the worktable.
  • Page 117 Purified nucleic acids are collected in 1.5 ml elution tubes. UV lamp Produces UV light with wavelength of 253.7 nm, corresponding to ultraviolet light type C. Capacity Up to 6 samples per run EZ1 Advanced User Manual 05/2008...

  • Page 118: Waste Electrical And Electronic Equipment (Weee

    QIAGEN, recycling can be provided upon request at additional cost. To recycle electronic equipment, contact your local QIAGEN sales office for the required return form. Once the form is submitted, you will be contacted by QIAGEN either to request follow-up information for scheduling collection of the electronic waste or to provide you with an individual quote.

  • Page 119: Ec Declaration Of Conformity

    Name and address of the company QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden Germany We herewith declare that the product EZ1 Advanced, cat. no. 9001410 – 11 meets all applicable requirements of the following European Directives Low Voltage Directive (LVD) 2006/95/EC Electromagnetic Compatibility...
  • Page 120 Appendix A This page intentionally left blank EZ1 Advanced User Manual 05/2008...

  • Page 121: Liability Clause

    Appendix B Appendix B Liability clause QIAGEN shall be released from all obligations under its warranty in the event repairs or modifications are made by persons other than its own personnel, except in cases where the Company has given its written consent to perform such repairs or modifications.
  • Page 122 Appendix B This page intentionally left blank EZ1 Advanced User Manual 05/2008...

  • Page 123: Index

    Reagent cartridges, 3-1, 3-9 loading, 5-10 Glossary, 8-1 Reagent wells, 3-10 Report file, 5-32 Heating positions, 3-10 Heating system, 3-1, 3-10 Safety biological, 1-4 chemical, 1-6 electrical, 1-3 Magnet, 3-11 heat hazard, 1-7 Magnetic particles, 3-1 EZ1 Advanced User Manual 05/2008 Index-1...
  • Page 124 5-29 serial port, 5-28 time, 5-27 VFD, 3-2, 3-3 Test, 5-20 axis, 5-21 Waste disposal, 1-6, A-6 heating block, 5-22 Worktable, 3-1, 3-6 serial port, 5-23 setting up, 5-9 version, 5-26 Tip adapters, 3-11 Index-2 EZ1 Advanced User Manual 05/2008...
  • Page 126 Australia Orders 03-9840-9800 Fax 03-9840-9888 Technical 1-800-243-066 Austria Orders 0800/28-10-10 Fax 0800/28-10-19 Technical 0800/28-10-11 Belgium Orders 0800-79612 Fax 0800-79611 Technical 0800-79556 Canada Orders 800-572-9613 Fax 800-713-5951 Technical 800-DNA-PREP (800-362-7737) China Orders 0086 21 3865 3865 Fax 0086 21 3865 3965...

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