Positioning the borosilicate glass capillary tip
Having obtained a signal, you must adjust the tip position to maximize it.
Using the three-axis manipulator, you can adjust the tip position up and
down, left and right, forward and backward. As a starting point, set the tip so
that it is on the center line of the sampling cone and at a distance between two
and three times the diameter of the cone aperture. Typically this distance is
approximately 2 mm.
Capillary tip position:
Cone aperture diameter
For tuning instructions, see the MassLynx, Xevo TQ-S online help, "Tuning
manually for NanoFlow operation".
Restarting a stalled borosilicate glass capillary electrospray
Should the spray stop, you can restart it. To do so, in the Tune window, set
Capillary to 0 kV. Then adjust the three-axis manipulator so that, viewed
under magnification, the capillary tip touches the sample cone, and a small
piece of the borosilicate glass capillary shears off.
If necessary, you can also apply some NanoFlow gas pressure, to force a drop
of liquid from the capillary. Apply as much as 1.4 bar (20 psi). Doing so
induces the drop's appearance unless the capillary is blocked.
d
3d
2d
NanoFlow ESI source
3-25