Task 2. Set Up The Signal For Quantification - Agilent Technologies 6100 Series Familiarization Manual

Task 2. Set up the signal for quantification

In this exercise, you add an extracted ion chromatogram (EIC) to the list of
available signals for the method. Then you add this EIC to the Signal Details,
so you can automatically load and integrate signals for the rest of the caffeine
standards.
Steps
1 Open the data file CAFCAL01.D,
located in the MSDEMO folder.
2 Extract the major ion of caffeine.
3 Display the Calibration Toolset.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Detailed Instructions
a Select File > Load Signal.
b Navigate to the folder:
C:\CHEM32\1\DATA\MSDEMO.
c Select the data file CAFCAL01.D.
d If necessary, clear the check box for
Load using Signal Details.
e In the Signals box, click the signal
that begins with MSD1 TIC.
f Click OK.
a Select File > Extract Ions.
b For Ion 1, type 195.1.
c For Ion 2, type 195.1.
d Click OK.
• Click the Calibration
icon, which is near
the middle of the window.
Quantitative Data Analysis

Task 2. Set up the signal for quantification

Comments
• For other ways to load signals, see
the chapter on Data Analysis in the
Concepts Guide.
• The 195 ion is the (M+H)
6
+
ion.
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