Exercise 2. Examine Mass Spectra - Agilent Technologies 6100 Series Familiarization Manual

3 Qualitative Data Analysis

Exercise 2. Examine mass spectra

Exercise 2. Examine mass spectra
In this exercise, you learn to display mass spectra. You choose background
(reference) spectra that you can later subtract from the spectra of the peaks of
interest. You learn how to display a single spectrum and an averaged spectrum
for a peak.
Steps
1 Zoom in on the first peak in the
chromatogram.
2 Display the Spectrum Toolset.
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Detailed Instructions
a Click the icon to zoom in.
b Use the mouse pointer to
draw a rectangle around
the peak. Take care to include the
chromatographic baseline.
c Check that your peak looks similar
to the one below.
d Note the width of the peak at half
height. You will need this
information to set up the SIM
analysis in Chapter 4.
a Click the Spectrum
icon, which is near
the middle of the window.
b If there is not room under your
chromatogram window to display
spectra, use your mouse pointer to
reduce the height of the
chromatogram window.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Comments
• If you want to try again, you can
zoom back out. Do one of the
following:
• Double-click the chromatogram
window.
• Click the icon to zoom
out.