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2
Set Up and Run a Scan Method
Task 2. Acquire the data
Steps
3 Monitor the total ion chromatogram
and the UV chromatogram during
data acquisition.
4 Save the signals for the Online Plot
window.
5 When the analysis is done, view the
results.
26
Detailed Instructions
a From the Online Plot window, click
the Change button.
b In the list of Available Signals,
select DAD A: Signal=272,16
Reference=360,100 and click Add.
c In the list of available signals,
select MSD: Signal 1 and click Add.
d Monitor the MS signal to ensure a
stable baseline.
a In the Edit Signal Plot dialog box,
click the Apply to Method button.
b Save the method.
• To view the results, go to the next
exercise.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Comments
• If the baseline fluctuation for the
MS signal is greater than 10%, the
nebulizer and source chamber may
require maintenance. See the
Agilent 6100 Series Single Quad
LC/MS System Maintenance
Guide.
• The C18 column may require one
or two injections of the sample to
be fully conditioned. During these
initial injections, everything may be
eluted from the column in the void
volume. Repeat the process and
separation will occur.
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