Table of Contents
Advertisement
1
Prepare for the Analysis
Task 3. Prepare the column for the analyses
Steps
5 While the column equilibrates, set
parameters for the MS spray
chamber so it can heat and
equilibrate as well.
•
Drying gas flow: 8 L/min
•
Nebulizer pressure: 35 psig
•
Drying gas temperature: 300
•
Capillary voltage: 3000 V
For 6150 with Agilent Jet Stream
technology:
•
Sheath Gas Flow: 12 L/min
•
Sheath Gas Temp: 360°C
•
Nozzle Voltage: 0 V
6 Reconnect the column to the DAD
and MS.
12
Detailed Instructions
a Right-click the MSD
icon on the system
diagram and select
Spray Chamber.
b Enter the parameters described in
step
5.
o
C
c Click OK.
d Wait 10 minutes before you tune
the MS.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Comments
• You can complete
"Exercise 3.
Check the current MS tune values
and adjust if necessary"
or without the column connected to
the DAD and MS, but you do need
to reconnect prior to the exercises
in
Chapter
2, "Set Up and Run a
Scan Method."
either with
Advertisement
Table of Contents
