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3
Qualitative Data Analysis
Exercise 3. Integrate the chromatogram
Steps
5 Save the integration events to the
method in memory.
6 Use the integrated chromatogram as
the basis for a faster way to display
background-subtracted spectra.
40
Detailed Instructions
• Click the icon to exit and
save the integration results.
a Click the Spectrum
icon.
b Click the icon to display
the Spectral Options dialog
box.
c Click the MS Reference tab.
d Under Reference Spectrum, click
Automatic.
e Click OK.
f Click the icon to get a
mass spectrum at the peak
apex.
g In the chromatogram window, click
somewhere on the fourth peak to
get the spectrum.
h Verify that the spectrum is similar
to the one shown below.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Comments
• To save the events to the method
on disk, you also need to save the
method to disk, as described in
step 3
on
page
42.
• When you set Reference
Spectrum, to Automatic, the
software automatically takes the
reference spectra for each peak, as
described in the Spectral Options
dialog box.
• The icon to get the mass spectrum
at the peak apex is available only if
you have integrated the
chromatogram. No matter where
you click on the peak, it gets the
spectrum at the apex. With this
tool, you may not need to zoom in
on the chromatogram to achieve a
precise location for the spectrum.
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