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1
Prepare for the Analysis
Task 3. Prepare the column for the analyses
Steps
8 After the bubbles are gone and the
purge is complete, enter a flow of
1 mL/min and 100% B.
9 Purge a short while longer, and then
close the purge valve.
Task 3. Prepare the column for the analyses
In the exercises in the next chapters, you analyze a mixture of four
sulfonamide compounds. To perform the analyses in the following chapters,
you must first condition and equilibrate your column.
Steps
1 Disconnect the column from the
detector and MS.
2 Flush the column with 100%
methanol at 1 mL/min (5 to 10 min).
•
ZORBAX SB-C18, 2.1 mm
3.5 µm, p/n 873700-902
10
Detailed Instructions
a Right-click the pump icon.
b Select Method.
c Enter the new parameters in
and click OK.
a Continue to purge for a short while.
b Close the black valve.
Detailed Instructions
a Turn the pump off by clicking
the red button in the Binary
Pump pane.
b Disconnect the column from the
detector and MS.
c Place the open end of the tubing
that exits the column into the
beaker.
a Turn the pump on.
b Flow methanol through the column
30 mm,
under the conditions used in Task 2,
step
8.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Comments
step
8,
For more information on purging the
pump, see the reference manual that
you received with your pump.
Comments
• To prevent detector contamination,
allow the column effluent to go
directly to the waste beaker.
• The data sheet shipped with the
column cartridge recommends that
you flush with 20 to 30 column-
volumes of 100% methanol
(approximately 5 to 7.5 mL).
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