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2
Set Up and Run a Scan Method
Task 2. Enter MS acquisition parameters
Task 2. Enter MS acquisition parameters
Steps
1 Enter parameters for the quadrupole
mass spectrometer (MS):
•
Signal 1, scan mode, positive
polarity
•
Scan range: 100 to 500
•
Fragmentor:
100 V for the Agilent 6120;
125 V for the 6130 or 6150
•
Gain: 1.00
•
Threshold: 150
•
Stepsize: 0.10
•
Peakwidth: 0.05 min
•
Scan data storage: Condensed
•
Active signals: 1 only
20
Detailed Instructions
a Right-click the MSD
icon on the system
diagram and select Set
up MSD Signals.
b Enter the parameters described in
step 1
and shown in the figure
below. Take care to enter the
appropriate Fragmentor voltage for
your MS model.
c Click OK.
Set to 100 for 6120
Set to 125 for 6130 or 6150
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Comments
• To save disk space you usually
acquire line spectra (Scan Data
Storage = Condensed). However,
when you acquire spectra from
intact proteins or protein
digests/peptides, you must acquire
and deconvolute profile spectra.
(Scan Data Storage = Full).
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