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Agilent 6100 Series Quadrupole LC/MS System
Familiarization Guide
4
Set Up and Run a SIM Method
Exercise 1. Set up a SIM acquisition method
Task 1. Load the scan method you created previously
Task 2. Enter MS acquisition parameters
Exercise 2. Acquire data with the SIM method
Task 1. Enter sample information
Task 2. Acquire the data
These exercises show you how to set up a data acquisition method that uses
selected ion monitoring (SIM). You set up the method for the demonstration
sample (sulfa mix) and then run the sample with that method.
To set up the SIM method, you modify the scan method that you created in
Chapter
2. To set up the SIM acquisition, you need the following for each of the
four sulfa compounds:
• The LC retention time
• The masses of ions in the spectrum
You get that information from the report you generated in
Before you start
• Complete the previous exercises in this manual.
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