Prepare For The Analysis - Agilent Technologies 6100 Series Familiarization Manual

Agilent 6100 Series Quadrupole LC/MS System
Familiarization Guide
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Prepare for the Analysis

Exercise 1. Prepare the LC to run the sample
Task 1. Start up ChemStation
Task 2. Purge the pump
Task 3. Prepare the column for the analyses
Exercise 2. Prepare the samples for the analyses
Exercise 3. Check the current MS tune values and adjust if necessary
This chapter presents exercises to help you learn how to:
• Prepare the LC and column for an analysis
• Prepare the samples that you analyze in these exercises
• Check the tune settings of the MS and adjust if necessary.
Before you start
• Order the sample: Agilent Electrospray LC Demo Sample, p/n 59987-20033.
• Order the column: Agilent ZORBAX SB-C18, 2.1 mm x 30 mm, 3.5 μm, p/n
873700-902.
• You may use another similar column, but you may need to adjust the
HPLC conditions to obtain good separation.
• Make sure that the electrospray source is installed.
• Read the Agilent 6100 Series Quadrupole LC/MS Systems Quick Start
Guide and Chapter 2 of the Agilent 6100 Series Quadrupole LC/MS Systems
Concepts Guide.
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