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1
Prepare for the Analysis
Exercise 2. Prepare the samples for the analyses
Steps
1 Prepare a 1:10 dilution of the original
sample in a 1-mL autosampler vial.
•
Final concentration is 10 ng/µL
•
You will use this sample for the
scan analysis in
Chapter
the SIM analyses in
Chapter
5.
2 Prepare a 1:20 dilution of the original
sample in a 1-mL autosampler vial.
•
Final concentration is 5 ng/µL
•
You will use this sample for the
SIM analysis in
Chapter
3 Prepare a 1:100 dilution of the
original sample in a 1-mL
autosampler vial.
•
Final concentration is 1 ng/µL
•
You will use this sample for the
SIM analysis in
Chapter
4 Prepare a solvent blank in a 1-mL
autosampler vial.
•
You will use this sample for the
SIM analysis in
Chapter
14
Detailed Instructions
a Transfer 100 µL of the sulfa mixture
into the autosampler vial.
b Add 900 µL of 90:10 water:methanol
that contains 5 mM ammonium
2, and for
formate (NH
Chapter 4
and
c Cap the vial.
a Transfer 50 µL of the sulfa mixture
into the autosampler vial.
b Add 950 µL of 90:10 water:methanol
that contains 5 mM ammonium
5.
formate.
c Cap the vial.
a Transfer 10 µL of the sulfa mixture
into the autosampler vial.
b Add 990 µL of 90:10 water:methanol
that contains 5 mM ammonium
formate.
5.
c Cap the vial.
a Into the autosampler vial, transfer
990 µL of 90:10 water:methanol that
contains 5 mM ammonium formate.
5.
b Cap the vial.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
HCO
.
4
2)
Comments
• The original sulfa mixture is
dissolved in a solvent mixture of
70% water and 30% acetonitrile.
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