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4
Set Up and Run a SIM Method
Task 2. Enter MS acquisition parameters
Steps
3 Set up the remaining SIM ions, using
the masses (to the nearest 0.1) that
you observed in the spectra from your
scan analysis:
•
Sulfachloropyridazine: Time 1.3,
SIM Ions 285, 287, and 156.
•
Sulfamethazine: Time 2.3,
SIM Ions 279 and 186.
•
Sulfadimethoxine: Time 3.3,
SIM Ions 311 and 156.
46
Detailed Instructions
a Click Add Grp, and type the name,
start time and mass (approximately
285) for sulfachloropyridazine.
b Click Add Ion, and type the mass for
the sulfachloropyridazine 156 ion.
c Click Add Ion, and type the mass for
the sulfachloropyridazine 287 ion.
d Repeat these steps until you have
entered two or three ions for each
of the remaining compounds.
e Click OK to close the Set Up MSD
Signals dialog box.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Comments
• Alternatively, instead of making
separate groups for each
compound as described here, all of
the SIM ions could be entered into
"Group 1", which could be
re-named "Sulfonamides". The first
SIM group can contain up to 100
ions.
• You may need to adjust the start
time for each SIM group. Refer to
your printout from
Chapter 3
determine a start time so that each
group change occurs about midway
between the chromatographic
peaks.
• If the retention time difference
between sulfachloropyridazine and
sulfamethazine is less than 0.3
minutes, merge these ions into one
group.
• The sulfachloropyridazine
additionally includes the chlorine
isotope at m/z 287.
to
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