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Task 2. Purge the pump
Use these instructions with the binary and quaternary pumps. See the
ChemStation online Help for instructions for the capillary and nanoflow
pumps.
Steps
1 Display the Method and Run Control
view.
2 Place the pump in standby mode.
3 Prepare solvents used in these
familiarization exercises.
•
A – 5 mM ammonium formate in
water
•
B – 5 mM ammonium formate in
methanol
4 Replace the solvent bottles with the
ones you just prepared.
5 Open the purge valve.
6 Enter a flow of 5 mL/min and
50% B, using water in channel A and
methanol in channel B.
7 Turn the pump on and monitor the
tubing for bubbles.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Detailed Instructions
• In the view selection area in the
lower left, click Method and Run
Control.
a Click More Binary Pump > Control
on the Instrument menu to open
the Pump Control dialog box.
b Select Standby and click OK.
a Into a 1-liter reservoir of
HPLC-grade water, add 1 mL of 5 M
ammonium formate.
b Into a 1-liter reservoir of
HPLC-grade methanol, add 1 mL of
5 M ammonium formate.
• Replace the bottles for channels A
and B.
a Turn the black purge valve on the
front of the pump
counter-clockwise two turns.
b Place the tubing that exits the
pump into a 250-mL or larger
beaker.
a Right-click the pump icon.
b Select Method.
c Enter the parameters in
click OK.
a To turn the pump on, click the
green button in the Binary
Pump pane.
b Monitor for bubbles.
Prepare for the Analysis
Task 2. Purge the pump
Comments
Alternate method:
• Select Standby from the Pump
shortcut menu.
• The part number for ammonium
formate is G1946-85021.
• Each ampoule contains 2.2 mL of
ammonium formate solution.
• Be sure to use HPLC-grade
solvents.
step 6
and
• Purge for about 3 minutes to pass
3X the volume for the binary pump.
• If you wish, you may purge each
channel individually first, to ensure
that neither is air-locked.
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