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Steps
7 Get a single background-subtracted
spectrum for the first LC peak.
8 Get a average background-subtracted
spectrum for the first LC peak.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Detailed Instructions
a Click the icon to get a
mass spectrum at any time
point.
b In the chromatogram window, click
somewhere on the peak to get the
spectrum.
c If necessary for easier viewing,
adjust the size and location of the
window labeled MS Spectrum.
d Verify that the spectrum is similar
to the one shown below.
a Click the icon to get an
averaged mass spectrum.
b In the chromatogram
window, click and drag the mouse
across the peak, as shown below.
c View the average spectrum in the
window labeled MS Spectrum.
Qualitative Data Analysis
Exercise 2. Examine mass spectra
Comments
• Under the conditions used to
acquire the demo data file
(mssulfas.d), the compounds elute
in the following order:
Sulfamethizole, m/z = 271
Sulfachloropyridazine, m/z = 285
Sulfamethazine, m/z = 279
Sulfadimethoxine, m/z = 311
• Depending on the organic mobile
phase and the modifiers, the elution
order for the 279 and 285 may
change.
• When a chromatographic peak
consists of a single compound, an
average spectrum is usually more
accurate.
3
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