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Steps
5 Enter pump parameters.
6 Set up the gradient timetable:
Line 1 Time 1:00, %B=12, Flow=0.4
Line 2 Time 3:00, %B=100, Flow=0.4
Line 3 Time 6:00, %B=100, Flow=0.4
Line 4 Time 7:00, %B=12, Flow=0.4
7 Enter a column compartment
o
temperature of 40
C.
8 Enter parameters for the diode-array
detector (DAD).
9 Select Data Acquisition only in the
Run Time Checklist.
10 Save the new parameters to the
method file, SULFA MS SCAN 1.M.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Detailed Instructions
a Click the Binary Pump tab on the
Setup Method dialog box.
b Set the parameters as follows:
Flow=0.400mL/min
StopTime=7.00 min
PostTime=3.00min
Solvent A=5mM ammonium
formate in water
Solvent B=5mM ammonium
formate in methanol
a Open the Timetable area in the
lower part of the tab, click Add, and
type the first line.
b Click Add and type the second line.
c Repeat
step b
for lines 3 and 4.
a Click the Column Comp. tab on the
Setup Method dialog box.
b Click the option button for
o
c Type 40.0 for
C.
a Click the DAD tab on the Setup
Method dialog box.
b Enter the parameters shown below:
•
Use Signal A: Wavelength 272nm,
Bandwidth16 nm
•
Reference Wavelength: 360 nm,
Reference Bandwidth 100 nm
•
Spectrum Store: All in peak
•
Peakwidth: > 0.10 min
c Click OK to close the Setup Method
dialog box with the new setpoints.
a Click Run Time Checklist on the
Method menu.
b Mark the Data Acquisition check
box.
c Click OK.
a Select File > Save > Method.
b In the box for Comment for method
history, type a comment.
c Click OK.
Set Up and Run a Scan Method
Task 1. Enter LC acquisition parameters
Comments
o
C.
• The DAD is used in this example,
but the variable wavelength
detector (VWD) may be used
analogously.
• While it is common to include Data
Analysis in the Run Time Checklist,
for these exercises, you will view
the results in
"Qualitative Data Analysis."
2
Chapter
3,
19
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