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Steps
3 Condition the column as follows,
using the solvents made up in Task 2,
step
6:
•
Flow rate – 0.4 mL/min
•
100% B for 1/2 hour
•
50% B for 1/2 hour
4 Equilibrate the column at the analysis
conditions:
12% B for 1/2 hour at 40 C
•
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Detailed Instructions
a Click Set up Instrument Method on
the Instrument menu to open the
Setup Method dialog box.
b Click the Binary Pump tab.
c Enter the flow rate in
step
d For Solvent B, type 100 and click
Apply.
e Wait 30 minutes.
f For Solvent B, type 50 and click
Apply.
g Wait 30 minutes.
a For Solvent B, type 12 and click
OK.
b Click the Column Comp. tab on the
Setup Method dialog box.
c For Temperature, type 40 and click
OK.
Prepare for the Analysis
Task 3. Prepare the column for the analyses
Comments
• At a flow rate of 0.4 mL/min, the
checkout column should produce
about 70 to 80 bar pressure
(measured without any fittings at
3.
the column exit).
• If, after you perform these steps,
the pump pressure through the
column is too high, order a
replacement SB-C18 column (p/n
873700-902).
• If your column is not new, you can
reduce the length of time that you
condition the column.
• While you condition and equilibrate
the column, you may complete
step 5
in this exercise and then
work on the rest of the exercises in
this chapter. Be sure to complete
step 6
before you go on to the next
chapter.
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