Task 2. Enter Ms Acquisition Parameters - Agilent Technologies 6100 Series Familiarization Manual

Task 2. Enter MS acquisition parameters

Steps
1 Enter the chromatographic peak
width for the SIM analysis.
2 Set up the first SIM ions using the
masses (to the nearest 0.1) that you
observed in the spectra from your
scan analysis:
• Sulfamethizole: Time 0,
SIM Ions 271 and 156.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Detailed Instructions
a Right-click the MSD
icon on the system
diagram and select Set
up MSD Signals.
b When the Set Up MSD Signals
dialog box is displayed, type 0.05
for Peakwidth.
a Under MSD Signal Settings, Signal
1, for Mode, select SIM.
b In the table, for Fragmentor, type
one of the following:
• 150 for the Agilent 6120
• 200 for the Agilent 6130 or 6150
c In the table, change Group 1 to
Sulfamethizole, and for SIM Ion,
refer to the spectrum on your
printout and type the mass (to the
nearest 0.1) for the 271 ion.
d Click Add Ion, and type the mass for
the sulfamethizole 156 ion.
Set Up and Run a SIM Method
Task 2. Enter MS acquisition parameters
Comments
• The peak width is an important
setting. It is used to calculate the
appropriate SIM dwell times to
deliver sufficient points across a
chromatographic peak to give good
quantitation.
• Peak width is defined as the full
width at half maximum (FWHM),
the width at 50% of the peak
height.
• In this example, each SIM group
includes a pseudo-molecular ion
and one fragment ion for
confirmation.
• Note that the figure below does not
show the fourth sulfa drug.
4
45