Exercise 3. Check The Current Ms Tune Values And Adjust If Necessary - Agilent Technologies 6100 Series Familiarization Manual

Exercise 3.
Check the current MS tune values and adjust if necessary
The MS is very stable and does not need to be tuned very often. You can
usually tune just once a month, or once a week at most. You can use the Check
Tune program described in this exercise to confirm that the MS is in
adjustment.
Steps
1 Switch to the MSD Tune view.
2 Select the tune file.
3 Run a Check Tune.
4 If Check Tune report suggests that
you adjust peak widths or mass axis,
then do that.
5 If the Check Tune report shows poor
sensitivity, which indicates that your
MS settings are significantly out of
adjustment, then run a full Autotune.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide

Exercise 3. Check the current MS tune values and adjust if necessary

Detailed Instructions
• In the view selection area in the
lower left, click MSD Tune.
a In the Select Tune File dialog box,
select ATUNES.TUN.
b Keep the default of Positive
Polarity (Standard).
c Click OK.
d In the status bar near the top of the
MSD Tune view, verify that you see
the following:
• Mode is API-ES
• Source is ESI (electrospray)
• From the Tune menu, select Check
Tune.
Note that Check Tune requires
values for comparison that are
determined from a previous
Autotune. Autotune is normally run
during installation.
a From the Tune menu, select Adjust
Mass Peak Width.
b From the Tune menu, select
Calibrate Mass Axis.
• From the Tune menu, select
Autotune > Positive Polarity.
Prepare for the Analysis
Comments
• Make sure that you use an
appropriate calibrant with an
appropriate source.
• Check Tune is normally all that you
need to do to confirm that the MS
settings are correct.
• If Check Tune indicates a problem
with your MS settings, then
proceed to step 4 and/or
• The exercises in this manual use
only the positive ion mode and
standard scan speeds, so it is not
necessary to tune for negative
polarity or fast scan.
1
step 5.
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