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Agilent Technologies 6820 Getting Started

Gas chromatograph
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沪 00000201号
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Agilent 6820 Gas
Chromatograph
Getting Started
Agilent Technologies

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  • Page 1 沪 00000201号 制 Agilent 6820 Gas Chromatograph Getting Started Agilent Technologies...
  • Page 2 Notices © Agilent Technologies, Inc. 2003, 2004, Warranty Safety Notices 2005 The material contained in this docu- No part of this manual may be reproduced in ment is provided “as is,” and is sub- C A U T I O N...

  • Page 3: Table Of Contents

    Contents The 6820 Gas Chromatograph Important Safety Warnings Many internal parts of the GC carry dangerous voltages Electrostatic discharge is a threat to GC electronics Many parts are dangerously hot Fibrous insulation Hydrogen Electron capture safety ECD warnings Safety precautions when handling ECDs...
  • Page 4 Two-Stage Pressure Regulators Traps Valve Actuator Air Installing the GC Gas Plumbing Step 1. Place the GC on the Bench and Open the Oven Door Step 2. Connect Carrier, Detector and Valve Actuator Gases and Check for External Leaks Install traps in the gas supply tubing Attach tubing to the inlet and detector fittings Check the gas fittings for leaks Attach valve actuator air (optional)
  • Page 5 Step 9. Connect the Appropriate Cables Other cables Other communications issues LAN cable RS-232 cable, G1530-60600 Agilent analog signal cable, G1530-60570 Agilent remote start/stop cable, 03396-61010 General purpose analog signal cable, G1530-60560 General purpose remote start/stop cable, 35900-60670 Agilent integrator signal inputs Step 10.
  • Page 6 Step 13. Compare Results to the Expected Chromatogram Typical FID checkout chromatograms Typical TCD checkout chromatograms Typical ECD checkout chromatograms Typical NPD checkout chromatogram Swagelok Connections Making Swagelok Connections Using a Swagelok Tee Factory Plumbing FID / FID TCD / TCD ECD / ECD NPD / NPD ECD/ FID...
  • Page 7 Your 6820 User Information Products Available Learning Products The learning products for the Agilent Technologies 6820 Gas Chromatograph (GC) consist of four manuals and a poster. Use the poster as a guide during installation of your new instrument. The five manuals are provided in a printable format...
  • Page 8 • How the GC provides feedback to you about its performance • How to program analysis settings • How to program the 6820 to automate tasks • How the GC components (inlet, detector, column oven, etc.) can impact your analysis •...
  • Page 9 When You Need More When you are ready to learn more about your instrument or if the instrument is in need of maintenance, refer to the following Information manuals as needed. The information in them is arranged so that you can learn at your own pace, reading only the information you need.

  • Page 10: The 6820 Gas Chromatograph

    In This Guide… This document is concerned with installing the 6820 Gas Chromatograph (the GC) in your laboratory, connecting it to your data recording or processing equipment, and verifying that it is operating correctly. The 6820 Gas Chromatograph A general description of the GC appears in this chapter. It includes general warnings (heat, shock, etc.) that apply to the...
  • Page 11 Safety and Regulatory Certifications Information Symbols Cleaning Recycling the Product The Agilent Technologies 6820 Gas Chromatograph is referred to as “the GC” throughout this manual. The GC is capable of up to two inlets and two detectors. Available inlets: •...

  • Page 12: Important Safety Warnings

    Important Safety Warnings Before moving on, there are several important safety notices that you should always keep in mind when using the 6820 GC. Many internal parts of the GC carry dangerous voltages If the GC is connected to a power source, even if the power switch is off, potentially dangerous voltages exist on: •...

  • Page 13: Many Parts Are Dangerously Hot

    The 6820 Gas Chromatograph Many parts are dangerously hot Many parts of the GC operate at temperatures high enough to cause serious burns. These parts include but are not limited to: • The inlets • The oven and its contents •...

  • Page 14: Hydrogen

    The 6820 Gas Chromatograph Hydrogen Hydrogen gas may be used as carrier gas, and/or as fuel for the FID. When mixed with air, hydrogen can form explosive mixtures. When using hydrogen (H ) as the carrier gas or fuel gas, be aware...

  • Page 15: Electron Capture Safety

    Electron capture safety ECD warnings Although beta particles at the energy level used in the 6820 ECD have little penetrating power —the surface layer of the skin or a few sheets of paper will stop most of them—they may be hazardous if the isotope is ingested or inhaled.

  • Page 16: Safety Precautions When Handling Ecds

    • Connect the ECD exhaust vent to a fume hood or vent it to the outside. Agilent Technologies recommends a vent line inside diameter of 6 mm (1/4-inch) or greater. With a line of this diameter, the length is not critical.

  • Page 17: Safety And Regulatory Certifications

    • AUS/NZ This ISM device complies with Canadian ICES-001. Cet appareil ISM est conforme a la norme NMB—001 du Canada. The 6820 GC is designed and manufactured under a quality system registered to ISO 9001. Information The Agilent Technologies 6820 Gas Chromatograph meets the...

  • Page 18: Symbols

    Failure to comply with these precautions violates safety standards of design and the intended use of the instrument. Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements. See accompanying instructions for more information.

  • Page 19: Cleaning

    The 6820 Gas Chromatograph Cleaning To clean the unit, disconnect the power and wipe down with a damp, lint-free cloth. Recycling the Product For recycling, contact your local Agilent sales office. Getting Started...
  • Page 20 The 6820 Gas Chromatograph Getting Started...
  • Page 21 Agilent 6820 Gas Chromatograph Getting Started Site Preparation Checklist Benchtop Space Requirements Venting toxic or noxious gases Electrical Requirements Operating Environment Gas Purity Carrier Gases Gas Plumbing Two-Stage Pressure Regulators Traps Valve Actuator Air This chapter describes the things you must do (or have done) before installing the GC in your laboratory.

  • Page 22: Site Preparation

    Site Preparation Checklist Use the checklist below to avoid missing anything. The page references on many of the items lead you to more detailed information. General Adequate bench space for GC and auxiliary equipment Appropriate operating environment (page Appropriate tools Appropriate supplies (page Correct electrical power...

  • Page 23: Benchtop Space Requirements

    Site Preparation Benchtop Space Requirements The GC is 68 cm (27 inches) wide, 50 cm (20 inches) high and 52 cm (21 inches) deep. You may need additional space for other instruments used with your GC. The GC is cooled by convection. Air enters vents in the side panels and underneath the instrument.

  • Page 24: Venting Toxic Or Noxious Gases

    Site Preparation Table 1 Dimensions, power, and weight Instrument Height Width Depth Maximum power Weight (VA) 6820 Gas 50 cm 68 cm 52 cm 2,950 for 200 V, 60 kg Chromatograph 20 inches 27 inches 21 inches 2,250 for all others 132 lb.

  • Page 25: Electrical Requirements

    Site Preparation Electrical Requirements Grounding A proper earth ground is required for GC operations. C A U T I O N To protect users, the metal instrument panels and cabinet are grounded through the three-conductor power line cord in accordance with International Electrotechnical Commission (IEC) requirements.
  • Page 26 Site Preparation Table 2 Line voltage requirements Voltage Maximum power Power line consumption (VA) requirement 120 V (±5%) 2,250 20-amp dedicated 200 V (±5%) 2,950 15-amp dedicated 220 V (±5%) 2,250 15-amp dedicated 230 V (±5%) 2,250 10-amp dedicated 240 V (±5%) 2,250 10-amp dedicated * Frequency range for all voltages is 48 to 66 Hz.

  • Page 27: Operating Environment

    Site Preparation Operating Environment Operating the GC within the recommended ranges (see Table ensures optimum instrument performance and lifetime. Table 3 Environmental limits Recommended temperature range Temperature range 20 to 27 °C 5 to 45 °C Recommended humidity range Humidity range 50% to 60% 5% to 90%, non-condensing Recommended altitude range...

  • Page 28: Gas Purity

    Site Preparation Gas Purity Some gas suppliers furnish “instrument” or “chromatographic” purity grades of gas that are specifically intended for chromatographic use. We recommend these grades for use with the GC. Generally, all gas supplies used should 99.995% to 99.9995% purity or better (Table 4).

  • Page 29: Carrier Gases

    Site Preparation Carrier Gases The carrier gas you use depends upon the type of detector and the performance requirements. Table 5 describes gases for both capillary and packed column use. Table 5 Carrier gas recommendations Detector Carrier gas Comments Thermal conductivity Helium General use Hydrogen...

  • Page 30: Gas Plumbing

    Site Preparation Gas Plumbing All compressed gas cylinders should be securely fastened to an immovable WA R N I N G structure or permanent wall. Compressed gases should be stored and handled in accordance with the relevant safety codes. Gas cylinders should not be located in the path of heated oven exhaust. To avoid possible eye injury, wear eye protection when using compressed gas.

  • Page 31: Two-Stage Pressure Regulators

    Site Preparation Two-Stage Pressure Regulators To eliminate pressure surges, use a two-stage regulator with each gas tank. Stainless steel, diaphragm-type regulators (Figure 1) are recommended. Figure 1 Two-stage pressure regulator The type of regulator you use depends upon gas type and supplier.

  • Page 32: Traps

    Site Preparation Traps For optimum sensitivity, install high-quality traps to remove traces of water and other contaminants. Moisture in carrier gas damages columns. Place a type 5A Molecular Sieve trap after the source regulator and before any other traps. Hydrocarbons create signal noise and ghost peaks. A hydrocarbon trap removes organics from gases.

  • Page 33: Valve Actuator Air

    Site Preparation Valve Actuator Air Some valves use pressurized air for actuation (others are electrically or manually driven). Actuator air must be free of oil, moisture, and particulates. It can be supplied from a dried regulated cylinder, although “house” air supplies or air from a compressor are acceptable.
  • Page 34 Site Preparation Getting Started...
  • Page 35 Agilent 6820 Gas Chromatograph Getting Started Installing the GC Gas Plumbing Step 1. Place the GC on the Bench and Open the Oven Door Step 2. Connect Carrier, Detector and Valve Actuator Gases and Check for External Leaks Install traps in the gas supply tubing...

  • Page 36: Installing The Gc

    If you are not sure how to make a Swagelok connection, see Appendix A, “Swagelok Connections” for instructions. Front and back views of the 6820 GC are shown in Figure 2 Figure Hydrogen is a flammable gas. If hydrogen or any other flammable WA R N I N G gas is used, periodic leak tests should be performed.
  • Page 37 Installing the GC Flow controls Display Front detector Back detector Front inlet Column oven door Keyboard Back inlet Figure 2 Front of the 6820 GC Oven exhaust ECD vents Detector gas inputs Communication connectors Carrier gas inputs Valve air Power...

  • Page 38: Step 1. Place The Gc On The Bench And Open The Oven Door

    Installing the GC Step 1. Place the GC on the Bench and Open the Oven Door Unpacking 1 Inspect the shipping containers for damage. If a container is damaged or shows signs of stress, notify both the carrier and your local Agilent office. Keep all shipping materials for inspection by the carrier.

  • Page 39: Step 2. Connect Carrier, Detector And Valve Actuator Gases And Check For External Leaks

    Installing the GC Step 2. Connect Carrier, Detector and Valve Actuator Gases and Check for External Leaks Materials needed • 1/8-inch preconditioned copper tubing • Tubing cutter • 1/8-inch Swagelok nuts, front and back ferrules • Two 7/16-inch wrenches Before starting, see Appendix B, “Factory Plumbing,”...

  • Page 40: Install Traps In The Gas Supply Tubing

    Installing the GC Install traps in the gas supply tubing Materials needed: • 1/8-inch preconditioned copper tubing • Tubing cutter • 1/8-inch fittings, nuts, and ferrules Swagelok • Two 7/16-inch wrenches and one 1/2-inch wrench • Traps 1 Determine where you will install the traps in your supply tubing line.

  • Page 41: Attach Tubing To The Inlet And Detector Fittings

    Installing the GC Attach tubing to the inlet and detector fittings Plumbing connections are made on the back of the pneumatics carrier on the left side of the GC. The internal plumbing in the GC, as delivered from the factory, depends on the kind of detector (or detectors) on your instrument.

  • Page 42: Attach Valve Actuator Air (Optional)

    Installing the GC Attach valve actuator air (optional) Valves are driven by air actuators. Valves should have a dedicated air source; they cannot share detector air supplies. Valve actuator air is supplied through 1/4-inch plastic tubing. If your GC was ordered with valves, the plastic tubing will already be attached to the actuators and will extend from the back of the GC.

  • Page 43: Attach Ecd Vent Tubing (If Ecds Present)

    Installing the GC Attach ECD vent tubing (if ECDs present) There are two fittings at the top of the gas connection panel. They are plumbed internally to the exit vents of the ECDs. Connect tubing from these fittings to an exhaust hood. Getting Started...

  • Page 44: Step 3. Set The Gas Pressures And Purge The System

    Installing the GC Step 3. Set the Gas Pressures and Purge the System Set source pressures The pressure set at a tank regulator depends on these factors: • The pressure needed to achieve the highest flow rate you intend to use. The pressure/flow relationship depends on the column or device involved.

  • Page 45: Set Internal Regulator Pressures

    Installing the GC Set internal regulator pressures Open the door on the left side of the GC. This exposes a panel with three single-stage pressure regulators. See Figure Top regulator Middle regulator Bottom regulator Figure 7 Internal pressure regulators Set the internal regulator pressures as shown in Table Table 8 Internal regulator settings...

  • Page 46: Purge The Gas Supply Systems

    Installing the GC Table 8 Internal regulator settings (continued) Regulator settings, MPa (psi) Detectors Middle Bottom TCD / ECD 0.27 (40) 0.20 (30) TCD / NPD 0.20 (30) 0.17 (25) 0.27 (40) NPD / FID 0.27 (40) 0.17 (25) 0.27 (40) Purge the gas supply systems In this procedure, you may have hydrogen gas flowing into the oven WA R N I N G...
  • Page 47 Agilent 6820 Gas Chromatograph Getting Started Preparing for Operation Step 4. Install the Column Hanger and the Checkout Column Step 5. Prepare the Checkout Column Step 6. Connect the Checkout Column to the Inlet Step 6a. Connect the column to a split/splitless inlet Step 6b.

  • Page 48: Preparing For Operation

    Preparing for Operation Step 4. Install the Column Hanger and the Checkout Column A Series 530 µm capillary column has been supplied with your GC. This column can be used with any inlet or detector, and is required for initial instrument checkout. Agilent capillary columns are wound on wire frames and mount on a pair of brackets that slip into slots at the top of the oven interior.

  • Page 49: Step 5. Prepare The Checkout Column

    Preparing for Operation Step 5. Prepare the Checkout Column You must prepare your capillary column before installation. Proper preparation assures that the column end has no burrs or jagged edges and is not contaminated with graphite or other material. Materials required Column nut and ferrule Capillary column Column cutter...
  • Page 50 Preparing for Operation 2 Score the column using a glass scribing tool. The score must be square to ensure a clean break (Figure 10). Glass scribing tool Figure 10 Scribing the column 3 Break off the column end by supporting it against the column cutter opposite the scribe (Figure 11).

  • Page 51: Step 6. Connect The Checkout Column To The Inlet

    Step 6. Connect the Checkout Column to the Inlet The following pages contain instructions for installing the checkout column in both of the inlets for the 6820 GC. Select the procedures that apply to your GC. Connect the column only to the inlet for conditioning. This avoids contaminating the detector with material driven off of the column.

  • Page 52: Step 6A. Connect The Column To A Split/Splitless Inlet

    Preparing for Operation Step 6a. Connect the column to a split/splitless inlet The checkout liner and insert have been installed in the inlet at the factory. Materials required Column nut and ferrule Column cutter 1/4-inch wrench Metric ruler 1 Prepare the column. See page 49 for instructions. 2 Position the column so it extends 4 to 6 mm above the end of the ferrule (Figure...
  • Page 53 Preparing for Operation 3 Insert the column in the inlet and slide the nut and ferrule up the column to the inlet base. Finger tighten the column nut until it starts to grip the column (Figure 15). Insulation cover Figure 15 Initial tightening 4 Adjust the column position so that the septum on the column is even with the bottom of the column nut...
  • Page 54 Preparing for Operation 5 Tighten the column nut an additional 1/4 to 1/2 turn so that the column cannot be pulled from the fitting with gentle pressure (Figure 17). 1/4 turn Figure 17 Securing the column Getting Started...

  • Page 55: Step 6B. Connect The Column To A Purged Packed Inlet

    Preparing for Operation Step 6b. Connect the column to a purged packed inlet The checkout liner and insert have been installed in the inlet at the factory. If your insulation cup is not installed, begin at line 1. Otherwise, begin at line 4. Materials required Column nut and ferrule Column cutter...
  • Page 56 Preparing for Operation 2 Install the insulation cup, if needed. Push the cup spring to the right. Slide the cup over the inlet fitting so that the insulation at the top of the cup is flush against the oven roof (Figure 19).
  • Page 57 Preparing for Operation 5 Position the column so it extends above the end of the column nut by 1 to 2 mm (Figure 21). Slide the septum up to the nut to mark this length. 1 to 2 mm Move septum here Figure 21 Marking the column 6 Push the column up 1 cm and guide it into the inlet liner.
  • Page 58 Preparing for Operation 7 Tighten the column nut an additional 1/4 to 1/2 turn so that the column cannot be pulled from the fitting when gentle pressure is applied (Figure 23). 1/4 turn Figure 23 Securing the column Getting Started...

  • Page 59: Step 7. Connect Power And Turn On The Gc

    Preparing for Operation Step 7. Connect Power and Turn On the GC 1 Verify that the power switch is in the off position (Figure 24). Power switch = Off = On Figure 24 Power switch location 2 Plug the power cord into the back of the GC (Figure 25) and to a power receptacle.
  • Page 60 Preparing for Operation 3 The self-test diagnostic tests run automatically. To see the pass/fail message, wait for the test to end and press [Oven] [Temp] [On] If the screen displays Power on OK, turn the GC off and continue with the installation procedure. If you do not see this message, turn the GC off and call Agilent service.

  • Page 61: Step 8. Condition The Column

    Preparing for Operation Step 8. Condition the Column The checkout column is now installed in an inlet. Do not connect it to a detector at this time. Volatile impurities will be driven off the column during the conditioning N O T E process.
  • Page 62 Preparing for Operation The exact amount of flow is not critical for conditioning. We suggest N O T E dipping the free end of the column in water and turning the control until a steady stream of bubbles occurs. The exact flow 2 Purge the column with carrier gas for at least 15 minutes.

  • Page 63: Step 9. Connect The Appropriate Cables

    Preparing for Operation Step 9. Connect the Appropriate Cables Power (Figure 25) and signal cables (Figure 27) connect on the back of the instrument. The other cables can be connected later. Signal 1 Signal 2 Remote RS-232 Figure 27 Cable connections on the back of the GC Two cables are supplied with every GC: •...

  • Page 64: Other Cables

    Preparing for Operation Other cables These cables must be ordered separately, if needed: • G1530-60570 Analog signal cable for Agilent 3395B/3396C integrators (one for each signal) • 03396-61010 Remote cable for Agilent 3395B/3396C integrators • G1530-60560 General purpose analog signal cable (one for each signal) •...

  • Page 65: Lan Cable

    Preparing for Operation LAN cable Connects a GC and a Cerity Chemical computer using LAN communications. See Figure 28 below. LAN hub/switch Crossover LAN cable 5183-4648 LAN cable LAN cable 8121-0940 8121-0940 Computer Computer Figure 28 Connecting the GC and computer with a hub/switch (shown at left) or a crossover cable (shown at right).
  • Page 66 IP address: 000.000.000.000< Gateway: 000.000.000.000 2 Enter the IP address for your 6820. Enter the numbers separated by dots (.) and press [Enter]. The GC displays a message instructing you to power cycle the instrument. Do not power cycle yet.

  • Page 67: Cable, G1530-60600

    Preparing for Operation RS-232 cable, G1530-60600 Connects a GC signal output to an Agilent Cerity Chemical data system using RS-232 communications. See Figure Figure 29 RS-232 cable Connect the RS-232 and adapter cables to the GC and computer as shown in Figure 30 below.

  • Page 68: Agilent Analog Signal Cable, G1530-60570

    Preparing for Operation Table 10 RS-232 communications parameters and default values Parameter Default value for use with Cerity Chemical Baud rate 19200 Handshake UART Parity None Data bits Stop bits End of command Agilent analog signal cable, G1530-60570 Connects both GC signal outputs to Agilent 3395B/3396C integrators and to Agilent 35900C/D/E Analog to Digital Interfaces.

  • Page 69: Agilent Remote Start/Stop Cable, 03396-61010

    Preparing for Operation Agilent remote start/stop cable, 03396-61010 Remote start/stop is used to synchronize two or more instruments. For example, you might connect an integrator and the GC so that the [Start]/[Stop] buttons on either instrument control both of them. You can synchronize a maximum of ten instruments using Remote cables.

  • Page 70: General Purpose Remote Start/Stop Cable, 35900-60670

    Preparing for Operation Table 11 Cable pinouts, general purpose analog signal cable Wire color Signal name Brown 0 to 1 mV(–) White 0 to 1 V, 0 to 10 V(–) 0 to 1 mV(+) Black 1 V(+) Blue 10 V(+) Orange Ground General purpose remote start/stop cable, 35900-60670...

  • Page 71: Agilent Integrator Signal Inputs

    Preparing for Operation Table 12 Cable pinouts, general purpose remote start/stop cable Wire color Signal name Black Ground White Prepare (low true) Start (low true) Green Start relay Brown Start relay Blue No connection Orange Ready (high true input) Yellow Stop (low true) Violet No connection...

  • Page 72: Step 10. Connect The Checkout Column To The Detector

    Preparing for Operation Step 10. Connect the Checkout Column to the Detector The instructions for connecting the column to a detector depend on the detector type. • For FID or NPD, see “Step 10a. Connect the column to an FID or NPD" on page 72.
  • Page 73 Preparing for Operation 2 See Figure 36 for the rest of the procedure. Gently insert the column as far as possible into the detector (about 40 mm) until it bottoms; do not attempt to force it further. Follow it with the ferrule and column nut. Detector base Ferrule Brass nut...

  • Page 74: Step 10B. Connect The Column To A Tcd

    Preparing for Operation Step 10b. Connect the column to a TCD 1 Assemble the capillary column adapter. See Figure 1/4-inch ferrule 1/4-inch nut Capillary column adapter Figure 37 Assembling the capillary column adapter 2 Install the adapter assembly in the detector fitting and tighten finger-tight.

  • Page 75: Step 10C. Connect The Column To An Ecd

    4 Pull the column out 1 mm. Use a wrench to tighten the nut an additional 1/4-turn. The column should not move. If the column ID is smaller than 0.2 mm, refer to the Maintenance and N O T E Troubleshooting manual on the 6820 User Information CD-ROM. Getting Started...
  • Page 76 Preparing for Operation Detector fitting Makeup gas adapter 19 ± 1 mm with liner Column nut Septum Column Withdraw column 1-2 mm Figure 39 Install the checkout column in an ECD 5 Leak-test the installation at the column nut. If necessary, tighten fitting(s) further only enough to stop leakage.
  • Page 77 Agilent 6820 Gas Chromatograph Getting Started Checking Performance Tools and Consumables Step 11. Set the Checkout Conditions Detector flows Lighting the FID flame Carrier gas flow Entering checkout method setpoints Working with Cerity Chemical Working with an integrator FID checkout conditions...

  • Page 78: Checking Performance

    Checking Performance Tools and Consumables You will need the following items to check your GC performance: • Checkout column: DB-1, 15 m x 0.53 mm x 0.5 µm, part no. 125-1017 • Checkout sample • For FID, 18710-60170 • For TCD, 18710-60170 •...

  • Page 79: Step 11. Set The Checkout Conditions

    You may want to check them using an electronic or bubble flow meter. See the Operation manual on the 6820 User Information CD for details of flow measurement. Figure 40 shows the front of the FID flow module.

  • Page 80: Carrier Gas Flow

    If using Cerity Chemical software, install it now. It will set most GC setpoints for you. Refer to the Operation manual on the 6820 User Information CD-ROM for information on: • How to use the GC with Cerity Chemical • How to set flows •...
  • Page 81 6 Move the cursor to the Init time line. 7 Type [1] [Enter]. 8 The three setpoints have been entered. See the 6820 Operation manual on the 6820 User Information CD-ROM for information on: • How to set flows • How to set program temperatures •...

  • Page 82: Fid Checkout Conditions

    Checking Performance FID checkout conditions Set up the GC as shown in Table 13. See page 79 for an example of setpoint entry. Table 13 FID checkout conditions Column DB-1, 15 m x 0.53 mm x 0.5 µm, part number 125-1017 Sample 1 µL of FID checkout, 18710-60170 Inlet...

  • Page 83: Tcd Checkout Conditions

    Checking Performance TCD checkout conditions Set up the GC as shown in Table 14. See page 79 for an example of setpoint entry. Table 14 TCD checkout conditions Column DB-1, 15 m x 0.53 mm x 0.5 µm, part number 125-1017 Sample 1 µL of FID checkout, 18710-60170 Inlet...

  • Page 84: Ecd Checkout Conditions

    Checking Performance ECD checkout conditions Set up the GC as shown in Table 15. See page 79 for an example of setpoint entry. Table 15 ECD checkout conditions Column DB-1, 15 m x 0.53 mm x 0.5 µm, part number 125-1017 Sample 1 µL of ECD checkout, 18713-60040 Inlet...

  • Page 85: Npd Checkout Conditions

    Checking Performance NPD checkout conditions Set up the GC as shown in Table 16. See page 79 for an example of setpoint entry. Table 16 NPD checkout conditions Column HP-5MS, 30 m x 0.32 mm x 0.25 µm, part number 19091S-413 Sample 1 µL of NPD checkout, 18789-60060 Inlet...

  • Page 86: Step 12. Analyze The Checkout Sample

    Checking Performance Step 12. Analyze the Checkout Sample 1 Enter the setpoints as described on the preceding pages. 2 Wait for the Not Ready light to go off. Prepare the sample 1 Score a sample vial at the neck and snap the top off. 2 Immediately transfer the sample to a screw-top or similar vial to retard evaporation.

  • Page 87: Step 13. Compare Results To The Expected Chromatogram

    Checking Performance Step 13. Compare Results to the Expected Chromatogram The chromatograms shown on these pages are typical for these detectors using the checkout column and the samples and conditions listed in this chapter. Your chromatogram will differ, particularly in the retention times, but the relative positions and peak sizes and shapes should resemble these examples.

  • Page 88: Typical Fid Checkout Chromatograms

    Checking Performance Typical FID checkout chromatograms Figure 41 FID, splitless injection Figure 42 FID, purged packed injection Getting Started...

  • Page 89: Typical Tcd Checkout Chromatograms

    Checking Performance Typical TCD checkout chromatograms Figure 43 TCD, splitless injection Figure 44 TCD, purged packed injection Getting Started...

  • Page 90: Typical Ecd Checkout Chromatograms

    Checking Performance Typical ECD checkout chromatograms R U N # J A N 2 8 , 2 0 0 3 1 3 : 0 2 : 1 5 S TA R T 4 . 9 8 3 6 . 5 9 9 S T O P Figure 45 ECD, splitless injection...

  • Page 91: Typical Npd Checkout Chromatogram

    Checking Performance Typical NPD checkout chromatogram Figure 47 NPD, splitless injection Getting Started...
  • Page 92 Checking Performance Getting Started...

  • Page 93: Swagelok Connections

    Agilent 6820 Gas Chromatograph Getting Started Swagelok Connections Making Swagelok Connections Using a Swagelok Tee The gas supply tubing is attached with fittings. If you Swagelok are not familiar with connections, review the following Swagelok procedures. Agilent Technologies...

  • Page 94: Making Swagelok Connections

    Swagelok Connections Making Swagelok Connections Objective To make a tubing connection that does not leak and that can be taken apart without damaging the fitting Materials needed: • 1/8-inch (or 1/4-inch, if used) preconditioned copper tubing • 1/8-inch (or 1/4-inch, if used) Swagelok nuts •...
  • Page 95 Swagelok Connections 3 Push the tubing into the stainless steel plug (Figure 49). 4 Make sure that the front ferrule is touching the plug. Slide the Swagelok nut over the ferrule and thread it onto the plug. Nut and ferrules Plug or fitting Tubing held in a vise...
  • Page 96 Swagelok Connections Pencil line Figure 51 Marking the fitting 8 For 1/8-inch Swagelok fittings, use a pair of 7/16-inch wrenches to tighten the fitting 3/4 of a turn (Figure 52). For 1/4-inch fittings, use a pair of 9/16-inch wrenches to tighten them 1-1/4 turn (Figure 52).
  • Page 97 Swagelok Connections 10 Both correctly- and incorrectly-swaged connections are shown in Figure 53. Note that the end of the tubing in a correctly-swaged fitting is not crushed and does not interfere with the action of the ferrules. Correct Figure 53 Completed fitting Getting Started...

  • Page 98: Using A Swagelok Tee

    Swagelok Connections Using a Swagelok Tee To supply gas from a single source to more than one input, use a Swagelok Tee. Do not combine valve actuator air with flame ionization air. The valve N O T E action will cause major upsets in the detector signal. Materials needed: •...

  • Page 99: Factory Plumbing

    Agilent 6820 Gas Chromatograph Getting Started Factory Plumbing FID / FID TCD / TCD ECD / ECD NPD / NPD ECD/ FID ECD/ NPD TCD / FID TCD / ECD TCD / NPD NPD / FID The gas plumbing connections on the back panel and inside the pneumatics carrier on the left side of the GC depend on the detector or detectors installed.
  • Page 100 Factory Plumbing Also note that: • The FID flow module contains an adjustable restrictor for the makeup (AUX) gases. • The TCD flow module contains adjustable restrictors for both the reference and makeup (AUX) gas. • The ECD flow module does not have adjustable restrictors. Flows are entirely pressure-controlled.

  • Page 101: Fid

    Factory Plumbing Figure 55 shows the factory configuration for one FID. Back panel Internal regulators Flow modules DETECTOR Front FID HYDROGEN HYDROGEN AUX GAS AUX GAS CARRIER FRONT Front inlet BACK Figure 55 Single flame ionization detector Installation notes • If using hydrogen as the carrier gas, you must supply nitrogen for the Aux Gas.

  • Page 102: Fid / Fid

    Factory Plumbing FID / FID Figure 56 shows the factory configuration for two FIDs. Back panel Internal regulators Flow modules DETECTOR Front FID HYDROGEN HYDROGEN AUX GAS AUX GAS Back FID CARRIER FRONT Front inlet HYDROGEN AUX GAS BACK Back inlet Figure 56 Dual flame ionization detector Installation notes...

  • Page 103: Tcd

    Factory Plumbing Figure 57 shows the factory configuration for one TCD. Back panel Internal regulators Flow modules DETECTOR Front TCD AUX GAS HYDROGEN AUX GAS CARRIER FRONT Front inlet BACK Figure 57 Single thermal conductivity detector Getting Started...

  • Page 104: Tcd / Tcd

    Factory Plumbing TCD / TCD Figure 58 shows the factory configuration for two TCDs. Back panel Internal regulators Flow modules DETECTOR Front TCD AUX GAS HYDROGEN AUX GAS Back TCD CARRIER AUX GAS FRONT Front inlet BACK Back inlet Figure 58 Dual thermal conductivity detectors Installation notes •...

  • Page 105: Ecd

    Factory Plumbing Figure 59 shows the factory configuration for one ECD. Back panel Internal regulators Flow modules DETECTOR Front ECD HYDROGEN ANODE PURGE AUX GAS AUX GAS CARRIER FRONT Front inlet BACK Figure 59 Single electron capture detector Installation notes •...

  • Page 106: Ecd / Ecd

    Factory Plumbing ECD / ECD Figure 60 shows the factory configuration for two ECDs. Back panel Internal regulators Flow modules DETECTOR Front ECD ANODE PURGE HYDROGEN AUX GAS AUX GAS Back ECD CARRIER ANODE PURGE FRONT Front inlet AUX GAS BACK Back inlet Figure 60...

  • Page 107: Npd

    Factory Plumbing Figure 61 shows the factory configuration for one NPD. Back panel Internal regulators Flow modules DETECTOR HYDROGEN HYDROGEN AUX GAS AUX GAS CARRIER FRONT Front inlet BACK Figure 61 Single nitrogen phosphorus detector Getting Started...

  • Page 108: Npd / Npd

    Factory Plumbing NPD / NPD Figure 62 shows the factory configuration for two NPDs. Back panel Internal regulators Flow modules DETECTOR Front NPD HYDROGEN HYDROGEN AUX GAS AUX GAS Back NPD CARRIER FRONT Front inlet HYDROGEN AUX GAS BACK Back inlet Figure 62 Dual nitrogen phosphorus detectors Getting Started...

  • Page 109: Ecd/ Fid

    Factory Plumbing ECD/ FID Figure 63 shows the factory configuration for one ECD and one FID. Back panel Internal regulators Flow modules DETECTOR Front ECD ANODE PURGE HYDROGEN AUX GAS AUX GAS Back FID CARRIER FRONT Front inlet HYDROGEN AUX GAS BACK Back inlet Figure 63...
  • Page 110 Factory Plumbing • If using nitrogen carrier for both inlets connect the source to AUX GAS and to the two carrier bulkhead fittings using two external tees. • If using helium or hydrogen for both carriers use external tee fittings for both carrier gas connections. •...

  • Page 111: Ecd/ Npd

    Factory Plumbing ECD/ NPD Figure 64 shows the factory configuration for one ECD and one NPD. Back panel Internal regulators Flow modules DETECTOR Front ECD ANODE PURGE HYDROGEN AUX GAS AUX GAS Back NPD CARRIER FRONT Front inlet HYDROGEN AUX GAS BACK Back inlet Figure 64...

  • Page 112: Tcd / Fid

    Factory Plumbing TCD / FID Figure 65 shows the factory configuration for one TCD and one FID. Back panel Internal regulators Flow modules DETECTOR Front TCD AUX GAS HYDROGEN AUX GAS Back FID CARRIER FRONT Front inlet HYDROGEN AUX GAS BACK Back inlet Figure 65...

  • Page 113: Tcd / Ecd

    Factory Plumbing TCD / ECD Figure 66 shows the factory configuration for one TCD and one ECD. Back panel Internal regulators Flow modules DETECTOR Front TCD AUX GAS HYDROGEN AUX GAS Back ECD CARRIER FRONT ANODE PURGE Front inlet BACK AUX GAS Back inlet Figure 66...

  • Page 114: Tcd / Npd

    Factory Plumbing TCD / NPD Figure 67 shows the factory configuration for one TCD and one NPD. Back panel Internal regulators Flow modules DETECTOR Front TCD AUX GAS HYDROGEN AUX GAS Back NPD CARRIER FRONT Front inlet BACK HYDROGEN AUX GAS Back inlet Figure 67 Thermal conductivity and nitrogen phosphorus detectors...

  • Page 115: Npd / Fid

    Factory Plumbing NPD / FID Figure 68 shows the factory configuration for one NPD and one FID. Back panel Internal regulators Flow modules DETECTOR Front NPD HYDROGEN HYDROGEN AUX GAS AUX GAS Back FID CARRIER FRONT Front inlet HYDROGEN AUX GAS BACK Back inlet Figure 68...
  • Page 116 Factory Plumbing Getting Started...
  • Page 118 Agilent Technologies © Agilent Technologies, Inc. Printed in China, April 2005 G1176-90010...

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