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5
Set Up and Run a Sequence
Exercise 2. Run the sequence
Exercise 2. Run the sequence
Now you are ready to acquire data with the sequence you just created.
Steps
1 Confirm that your sequence includes
four samples.
2 Place the samples you prepared in
Chapter 1
into the appropriate
positions in the autosampler.
3 Inject the samples.
4 (Optional) For the first blank analysis,
monitor the total ion chromatogram
and the UV chromatogram during
data acquisition.
5 When the sequence is done, view the
Sequence Summary Report.
6 When the sequence is finished, view
the results.
60
Detailed Instructions
• Verify that the Autosampler Tray
diagram shows four samples.
• Click the Sequence
start button on the
Run Control Bar.
a Activate the Online Plot window.
b Monitor the MS signal to ensure a
stable baseline.
a Retrieve the report from the printer.
b Examine the report to confirm that
all the samples ran.
a Display Data Analysis view.
b Load the first data file you just
created.
c Examine the DAD and MS
chromatograms.
d Repeat
step b
and
step c
other data files.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Comments
You prepared the samples in
2. Prepare the samples for the
analyses"
on page 13.
This button is only available if you
have selected Sequence mode on the
main toolbar.
As the sequence progresses, the
Autosampler Tray diagram is
color-coded as follows:
Gray - samples that have been
analyzed.
White - samples not yet analyzed.
Blue - the current sample.
• If you need help, follow the general
procedure in
and manipulate
chromatograms"
Chapter
3.
for the
• When you analyze your own
samples, you can set up your
method to automatically generate a
data analysis report for each
sample in the sequence.
"Exercise
"Exercise 1. Display
on page 28 in
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