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6
Quantitative Data Analysis
Task 5. Set up the calibration curve
Steps
4 Add the second standard to the
calibration curve.
5 Add the remaining three standards to
the calibration table:
•
CAFCAL03.D: 5 ng/µL
•
CAFCAL04.D: 25 ng/µL
•
CAFCAL05.D: 50 ng/µL
70
Detailed Instructions
a Click the icon to Add new
level.
b In the dialog box, for
Default Amount, type 1 and click
OK.
c Verify that the calibration table now
has two entries, and the calibration
curve contains two points.
a Select File > Load Signal.
b Under File name, select the next
data file.
c Verify that the chromatogram is
properly integrated.
d Click the icon to Add new
level.
e In the dialog box, for
Default Amount, type the amount
shown in
step 5
and click OK.
f Verify that the calibration table and
the calibration curve contain the
new entry.
g Repeat
step a
through
you have added all the standards.
h Confirm that your calibration table
and calibration curve look like the
ones below.
Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
Comments
• If multiple peaks are integrated in a
chromatogram, retention time is
used to find the correct peak for the
calibration curve.
step f
until
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