Reagent Prep; Protocol Set Up And Run - Agilent Technologies Magnis NGS Prep System Quick Reference Manual

Running Protocols
For instructions on manually decontaminating the surfaces of the instrument deck, consult the
full-length Magnis NGS Prep System User Guide (publication K1007-90000).

2) Reagent prep

Prepare the samples, target enrichment reagents, and other materials needed for the protocol run
according to the user manual for your specific Magnis Target Enrichment Kit. The user manual
contains details on the materials required to run a protocol and instructions on loading your DNA
samples into the Magnis Sample Input Strip.
The image below describes the orientation of the Magnis Sample Input Strips. Make sure to track
the locations of your samples as you load them into the strip.
Figure 6
C AU T I O N
3) Protocol set For detailed instructions on running a protocol for your particular sample type, refer to the user
up and run manual for your Magnis Target Enrichment Kit.
1 From the Home screen, press Run Protocol.
The system locks the instrument door and performs an IHC. At completion of the IHC, the
Enter Run Info step opens.
2 Complete the Run Setup steps and start the protocol run, as instructed in the user manual for
your Magnis Target Enrichment Kit.
The software walks you through the individual Run Setup steps
Enter Run Info step. Press the forward and back arrow buttons to navigate through the steps
(or, if using a USB-connected mouse, click the arrow buttons with the cursor). Because the
steps vary depending on the type or target enrichment you are running, you will need to refer to
the instructions and screen images provided in the user manual for your Magnis Target
Enrichment Kit.
After you start the run, the system's LED light turns green, indicating that a protocol run is in
progress. The LED changes from green to blue upon completion of a the run. Additionally, if your
account is set up to receive email alerts, the system sends you an email when the run is complete.
If the system encounters an error during the protocol, the LED light changes to red and, if your
account is configured for alerts, the system sends you an email notifying you of the error.
Magnis NGS Prep System Quick Reference Guide
Orientation of the Magnis Sample Input Strips
Do not add any writing or labels to the strips, plates, and other labware that
may obscure the barcodes.
(Figure 7), starting with the
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