Wavelength Verification And Recalibration - Agilent Technologies 1260 User Manual

Wavelength Verification and Recalibration

The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of
the deuterium lamp for wavelength calibration. The sharp emission lines
enable more accurate calibration than is possible with holmium oxide. When
verification is started, the 1-nm slit is moved into the light path automatically,
and the gain is set to zero. To eliminate effects due to absorbing solvents, the
test should be done with bubble free degassed HPLC water in the flow cell.
If a deviation is found and displayed, it can be recalibrated by pressing Adjust.
The deviations are tracked in the Calibration History (diagnosis buffer in the
detector).
Figure 71
Wavelength calibration should be done
• after maintenance of the flow cell,
• lamp exchange, or
• after a major repair, like processor board or optical unit exchange, see also
After calibration, the holmium oxide test (see
verification of wavelength accuracy at three additional wavelengths.
Agilent 1260 Infinity DAD and MWD User Manual

Wavelength Verification and Recalibration

"Replacing the Module's Firmware"
Wavelength Verification and Recalibration
on page 229.
Figure 68
Test Functions
on page 175) provides
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