8
Test Functions
Wavelength Verification-Calibration
Wavelength Verification-Calibration
Wavelength calibration of the detector is done using the zero- order
position and 656 nm emission line position of the deuterium lamp. The
calibration procedure involves two steps. First the grating is calibrated on
the zero- order position. The stepper- motor step position where the
zero- order maximum is detected is stored in the detector. Next, the
grating is calibrated against the deuterium emission- line at 656 nm, and
the motor position at which the maximum occurs is stored in the detector.
In addition to the zero- order and 656 nm (alpha- emission line)
calibration, the beta- emission line at 486 nm and the three holmium lines
are used for the complete wavelength calibration process. These holmium
lines are at 360.8 nm, 418.5 nm and 536.4 nm.
The wavelength verification/calibration takes about 2.5 min and is disabled within the first
N O T E
10 min after ignition of the lamp because initial drift may distort the measurement.
When the lamp is turned ON, the 656 nm emission line position of the
deuterium lamp is checked automatically.
The Wavelength Verification/Calibration is available in
• Agilent Lab Advisor (preferred tool).
• Agilent Instant Pilot G4208A, via More-Diagnosis-VWD-Calibration.
When to Calibrate the Detector
The detector is calibrated at the factory, and under normal operating
conditions should not require recalibration. However, it is advisable to
recalibrate:
• after maintenance (flow cell or lamp),
• after repair of components in the optical unit,
• after exchange of the optical unit or VWM board,
• at a regular interval, at least once per year (for example, prior to an
• when chromatographic results indicate the detector may require
108
Operational Qualification/Performance Verification procedure), and
recalibration.
Agilent 1260 Infinity VWD User Manual