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Agilent Technologies 1260 Infinity User Manual

Agilent Technologies 1260 Infinity User Manual

Infinity evaporative light scattering detector
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  • Page 1 Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual...
  • Page 2 Use, duplication or disclo- sure of Software is subject to Agilent Tech- nologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as Agilent 1260 Infinity ELSD User Manual...
  • Page 3 G4218A Agilent Evaporative Light Scattering Detector. It includes: Introduction Site Requirements and Specifications Site requirements and specifications of the Agilent 1260 Infinity Evaporative Light Scattering Detector Installing the Detector Installation of the detector. Using the System This chapter describes how to operate the Low Temperature Evaporative Light Scattering Detector.
  • Page 4 Detailed illustrations and lists for identification of parts and materials for repair The Control Panel This chapter describes the role of the various controls and displays on the detector. Appendix This chapter contains safety information. Agilent 1260 Infinity ELSD User Manual...

  • Page 5: Table Of Contents

    Location of the Detector in the Laboratory Environment Specifications Installing the Detector Lifting and Carrying the Module Unpacking the System Installing the Detector Gas Supply Vent the Exhaust Line to a Fume Hood Electrical Connections Additional Options Agilent 1260 Infinity ELSD User Manual...
  • Page 6 Column Treatment Optimizing the Filter Troubleshooting and Diagnostics Basics of Troubleshooting Initial Troubleshooting Activities If there is no response from the system Perform the Noise Tests Specific Detector Troubleshooting Noise test procedures Preliminary Activities Agilent 1260 Infinity ELSD User Manual...
  • Page 7 Decontaminating the detector Removing the nebulization chamber and nebulizer Cleaning the Nebulizer Adjusting stray light settings Parts for Repair Evaporative Light Scattering Detector Parts The Control Panel The Control Panel The Digital Display The User Interface Agilent 1260 Infinity ELSD User Manual...
  • Page 8 Contents Appendix Safety Information Solvent Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Lithium Batteries Information Agilent Technologies on Internet Index Agilent 1260 Infinity ELSD User Manual...

  • Page 9: Introduction

    Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual Introduction The Low Temperature Evaporative Light Scattering Detector Principle of Operation Agilent Technologies...

  • Page 10: The Low Temperature Evaporative Light Scattering Detector

    Introduction The Low Temperature Evaporative Light Scattering Detector The Low Temperature Evaporative Light Scattering Detector The Agilent 1260 Infinity Evaporative Light Scattering Detector (Figure 1) is designed to detect compounds in the eluent from high performance liquid chromatography (HPLC), micro-HPLC, gel permeation chromatography (GPC) or counter current chromatography (CCC).
  • Page 11 RS-232 port. The output can be sent to a recorder or data station. The detector includes a nebulizer, evaporation tube and detector head. The evaporation tube is located in an oven to assist in the evaporation of the solvent. Agilent 1260 Infinity ELSD User Manual...

  • Page 12: Principle Of Operation

    (PM). The degree of light scattering is related to the concentration of the compound of interest in the sample. A cross sectional view of the system is presented in Figure Agilent 1260 Infinity ELSD User Manual...
  • Page 13 Introduction Principle of Operation Figure 3 Schematic display of principle measurement steps in the ELSD of the Detector Agilent 1260 Infinity ELSD User Manual...

  • Page 14: Nebulization

    (the optimal range for each nebulizer is indicated Table 9). Additional nebulizers are available from Agilent Technologies and can be easily installed, see “Installing the Nebulizer and Nebulization Chamber Assembly”...
  • Page 15 Introduction Principle of Operation Peek inlet Colored Seal Ring Gas inlet Figure 4 Design of the Nebulizer Agilent 1260 Infinity ELSD User Manual...
  • Page 16 Introduction Principle of Operation Colored seal Nebulizer To HPLC system Nebulization chamber Inlet for nitrogen To evaporator and detection To waste Siphon Figure 5 The Nebulizer Agilent 1260 Infinity ELSD User Manual...

  • Page 17: Evaporation Of The Solvent

    COOH, CF COOH and HNO Detection The carrier gas transports the microparticles from the heating tube into the detection chamber (Figure Detection chamber Photomultiplier Light trap Addtitional gas Heating tube Figure 6 The Detection Chamber Agilent 1260 Infinity ELSD User Manual...
  • Page 18 A gas inlet before the detector chamber provides a concentric shield for the carrier gas. This serves to eliminate diffusion of the carrier gas and eliminates contamination of the detector cell. Agilent 1260 Infinity ELSD User Manual...

  • Page 19: Site Requirements And Specifications

    Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual Site Requirements and Specifications Site Requirements Specifications Agilent Technologies...

  • Page 20: Site Requirements

    WA R N I N G Shock hazard or damage of your instrumentation can result, if the devices are connected to a line voltage higher than specified. ⇒ Connect your instrument to the specified line voltage. Agilent 1260 Infinity ELSD User Manual...

  • Page 21: Gas Considerations

    => Never operate your instrumentation from a power outlet that has no ground connection. => Never use a power cord other than the power cord designed for your region Agilent 1260 Infinity ELSD User Manual...

  • Page 22: Exhaust Venting And Drain Requirements

    Use of unsupplied cables WA R N I N G The use of cables which haven’t been supplied by Agilent Technologies can lead to damage of the electronic components or personal injury. => Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

  • Page 23: Location Of The Detector In The Laboratory

    • Maximum humidity of 80% for temperatures under 31°C, with linear decrease to 50% at 40°C • Maximum variations for main power voltage: 10% from nominal voltage. • Transitory overvoltage of class II • Pollution degree: 2 Agilent 1260 Infinity ELSD User Manual...

  • Page 24: Specifications

    Signal Drift Less than 1 mV/h Signal Noise < ± 1 mV Signal Output 0-1000 mV (Analog) RS-232 (Digital) Inputs Remote Autozero (Contact Closure or TTL Signal) Remote Powerdown Mode (Contact Closure or TTL Signal) Agilent 1260 Infinity ELSD User Manual...
  • Page 25 Nebulizer Gas: Nitrogen, pressure 3.5 bar Gain: 12 Noise: measured as mean value for 10 segments of 1 minute. For each segment the difference of the highest minus the lowest peak is calculated and divided by 2. Agilent 1260 Infinity ELSD User Manual...
  • Page 26 Site Requirements and Specifications Specifications Agilent 1260 Infinity ELSD User Manual...

  • Page 27: Installing The Detector

    Installing the Nebulizer and Nebulization Chamber Assembly This chapter describes how the laboratory should be prepared to optimize the performance of the Agilent 1260 Infinity Evaporative Light Scattering Detector and indicates how the unit is interfaced to other devices such as the column and the data recording device.

  • Page 28: Lifting And Carrying The Module

    Once the module is unpacked, ensure that no cables or tubing are connected when you carry the module. The module should be lifted by the bottom (e.g. place your hands under the module). Two persons are needed to ensure easy transport and avoid bodily injury (Figure Agilent 1260 Infinity ELSD User Manual...
  • Page 29 Installing the Detector Lifting and Carrying the Module Do NOT Lift Here Do NOT Lift Here Carry Here Carry Here Figure 7 Carrying the System Agilent 1260 Infinity ELSD User Manual...

  • Page 30: Unpacking The System

    => It is recommended that the shipping carton be retained as it can be used if it should become necessary to transport the system. Table 2 Components Shipped with the Agilent 1260 Infinity Evaporative Light Scattering Detector Quantity Part Number...
  • Page 31 Installing the Detector Unpacking the System Table 2 Components Shipped with the Agilent 1260 Infinity Evaporative Light Scattering Detector Quantity Part Number Description 1 (500 cm) 0890-1727 Tubing 5m 5061-3378 Cable, remote (APG) 1 (optional) G4218-60100 Gas regulator with filter 0.01 µm and...

  • Page 32: Installing The Detector

    Accessory Kit consists of: 1 Power cable 1 Auto-zero cable 1 Signal cable 1 RS-232 cable 1 External event cable 6 mm O.D. gas tubing (2 meters + 1 meter sets) 1 set of replacement fuses Agilent 1260 Infinity ELSD User Manual...

  • Page 33: Gas Supply

    The unit is connected to the gas supply via the 6 mm plastic tubing (supplied) using the fitting on the upper left corner of the supply panel on the back of the detector (Figure Figure 8 Supply Panel Agilent 1260 Infinity ELSD User Manual...
  • Page 34 2 Cut the tube square. fitting as shown, after removing Parafilm™ from detector gas inlet. 3 Insert the tube into the fitting until it bottoms. 4 Pull the tube to check engagement of the grab Agilent 1260 Infinity ELSD User Manual...
  • Page 35 Make sure that no tube damage or inappropriate installation could allow a gas leak in laboratory. Agilent 1260 Infinity ELSD User Manual...
  • Page 36 Removing the Gas Inlet Tube To remove the gas inlet tube (if necessary) 1 dissengage the grab ring teeth by a simple manual pressure on the push sleeve and withdraw the tube from the fitting. Agilent 1260 Infinity ELSD User Manual...

  • Page 37: Vent The Exhaust Line To A Fume Hood

    Avoid long tube installations in upward direction creating condensation dropping back into the detector. Avoid loops or bends in the tubing which form condensation traps causing bad measurement results. Agilent 1260 Infinity ELSD User Manual...

  • Page 38: Electrical Connections

    All components of the chromatographic system should be connected to a common ground. If a two wire outlet is used, make sure that an adapter is used to connect the third wire to ground. Agilent 1260 Infinity ELSD User Manual...
  • Page 39 Installing the Detector Electrical Connections Figure 9 Supply panel Agilent 1260 Infinity ELSD User Manual...

  • Page 40: Additional Options

    If the external autozero function shall be used, plug the cable that is supplied into the Ext Autozero socket on the detector (Figure 10) and to the appropriate socket on the controlling device Use a contact closure or TTL signal for auto-zeroing the detector. Agilent 1260 Infinity ELSD User Manual...
  • Page 41 • RS-232 Port If a personal computer is used with the detector, the detector should be connected to the computer via the RS-232 port using the supplied cable. Agilent 1260 Infinity ELSD User Manual...
  • Page 42 All components of the chromatographic system should be connected to a common ground. If a two wire outlet is used, make sure that an adapter is used to connect the third wire to ground. Agilent 1260 Infinity ELSD User Manual...

  • Page 43: Installing The Nebulizer And Nebulization Chamber Assembly

    The installed Nebulizer/Nebulization chamber assembly is shown in Figure To Column Bulkhead Nebulizer Gas inlet Fitting Nebulization chamber Bracket Siphon Overflow Figure 11 Installing the nebulizer/nebulization chamber assembly Agilent 1260 Infinity ELSD User Manual...
  • Page 44 Teflon tubing with an outer green sleeve. For proper operation, the Teflon tubing must extend less than 2 mm past the end of the green sleeve (Figure 13). Figure 13 Nebulizer Inlet Fitting Agilent 1260 Infinity ELSD User Manual...
  • Page 45 If you are using a highly volatile solvent like hexane or CH2Cl2, use water to fill the overflow. The liquid should fill the bent part of the siphon, but should not pool in the bottom of the condenser tube. Agilent 1260 Infinity ELSD User Manual...

  • Page 46: Connecting The Siphon Overflow

    => Please observe appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the solvent vendor, especially when toxic or hazardous solvents are used. Agilent 1260 Infinity ELSD User Manual...

  • Page 47: Connecting The Nebulization Gas To The Nebulizer

    (which should be 0 or very close to it), the temperature (which should be the ambient temperature), the pressure (which should be zero or very close to it) and the gain. Refer to Chapter 4 for preparing the unit for routine operation. Agilent 1260 Infinity ELSD User Manual...
  • Page 48 Installing the Detector Installing the Nebulizer and Nebulization Chamber Assembly Agilent 1260 Infinity ELSD User Manual...

  • Page 49: Using The System

    Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual Using the System Preparing the System for Operation Auto-zeroing the Detector Routine Operation of the System Installation Test Procedure Powering Down and Shutting Down the System This chapter describes the operations that should be performed on a routine basis when you want to collect chromatographic data using the Agilent 1260 Infinity Evaporative Light Scattering Detector.

  • Page 50: Preparing The System For Operation

    5 Start the mobile phase flow through the system and allow the overall system to operate for at least 15 minutes to ensure that all components are equilibrated and a stable baseline is obtained. Please refer to Chapter 5 for choosing temperature settings, solvents and columns. Agilent 1260 Infinity ELSD User Manual...

  • Page 51: Auto-Zeroing The Detector

    If desired, the auto-zero command can be initiated by an external device such as the HPLC system controller. To employ this feature, a cable from the external device is plugged into the EXT AUTO ZERO socket on the rear panel. “Additional Options” on page 40. Agilent 1260 Infinity ELSD User Manual...

  • Page 52: Routine Operation Of The System

    Ensure that the siphon is filled with liquid at all times. The overflow from the siphon should be collected in a suitable waste container by using the drain tube. ✔ Never exceed a gas pressure of 4.5 bar (67 psi). Agilent 1260 Infinity ELSD User Manual...
  • Page 53 Avoid the use of solvent or samples that could corrode the detector. The mobile phase is in contact with glass and Teflon tubing and the evaporation tube is constructed from stainless steel. See “Solvent Information” page 112. Agilent 1260 Infinity ELSD User Manual...

  • Page 54: Installation Test Procedure

    ELSD pressure 3.5 bar (51 psi) ELSD gain System Agilent 1260 Infinity Standard LC System with Standard Degasser, Binary Pump, High Performance Autosampler, Thermostatted Column Compartment and Diode Array Detector After turning on the detector, allow 15 minutes for equilibration. Monitor the temperature using the Data System online signal display or the instrument display.
  • Page 55 A chromatogram similar to the one in the following figure can be expected. Please note that retention time, peak area and peak shape can vary and depend on the individual HPLC system configuration. Figure 14 Typical chromatogram for caffeine, see measurement conditions in text Agilent 1260 Infinity ELSD User Manual...

  • Page 56: Powering Down And Shutting Down The System

    If the LC system shall be used without the ELSD, it must be switched off and removed from the software configuration for avoiding problems due to a missing instrument (power failure). Agilent 1260 Infinity ELSD User Manual...
  • Page 57 3 Stop the mobile phase flow but let gas flow to dry to avoid particles deposit. 4 Stop the gas flow. 5 Shut down the detector. The time required for each step depends on the application, used solvents, type and concentration of samples and should be determined practically. Agilent 1260 Infinity ELSD User Manual...
  • Page 58 Using the System Powering Down and Shutting Down the System Agilent 1260 Infinity ELSD User Manual...

  • Page 59: Optimizing Performance

    Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual Optimizing Performance Selecting the Optimum Temperature Optimizing the Mobile Phase Optimizing the Filter Agilent Technologies...

  • Page 60: Selecting The Optimum Temperature

    35 °C and 60 °C (see “Installation Test Procedure” on page 54, Table 4). It is clear that the use of a low temperature provides significantly better sensitivity for volatile and thermally sensitive compounds. Agilent 1260 Infinity ELSD User Manual...
  • Page 61 Optimizing Performance Selecting the Optimum Temperature Figure 15 Chromatogram of Caffeine at Various Temperatures The minimum temperature that can be used depends on the flow rate and the nature of the mobile phase. Agilent 1260 Infinity ELSD User Manual...

  • Page 62: Optimizing The Mobile Phase

    (51psi) may have to be adjusted in order to optimize the background noise and the signal-to-noise ratio. Sample Pre-Treatment If the sample contains insoluble particles, it should be filtered through a 0.2 µm or 0.45 µm filter before injection. Agilent 1260 Infinity ELSD User Manual...

  • Page 63: Column Treatment

    This will flush out micro-particles that may reside in the column from the manufacturing or shipment process. It is suggested that you perform the Column Noise test (see “Column Noise Test” on page 77) to obtain the baseline signal value for the column. Agilent 1260 Infinity ELSD User Manual...

  • Page 64: Optimizing The Filter

    Signal to noise ratio is multiplied by 3 when choosing Filter 1S without any peak broadening effect. If Signal to Noise ratio is more important than resolution, a Filter 2S or higher can be set to improve sensitivity even more. Agilent 1260 Infinity ELSD User Manual...

  • Page 65: Troubleshooting And Diagnostics

    Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual Troubleshooting and Diagnostics Basics of Troubleshooting Specific Detector Troubleshooting Noise Test Procedures Troubleshooting Based on Diagnostic Tests Troubleshooting Based on Instrument Signals This chapter describes troubleshooting activities which are useful for determining the cause of wrong or unexpected.

  • Page 66: Basics Of Troubleshooting

    Troubleshooting and Diagnostics Basics of Troubleshooting Basics of Troubleshooting The Agilent 1260 Infinity Evaporative Light Scattering Detector is designed to be used with a liquid chromatography system. For troubleshooting, it is important to distinguish between detector issues and issues with other parts of the system, which might affect detector output.

  • Page 67: Initial Troubleshooting Activities

    4.5 bar maximum) and is stable. The gas filter should be clean and in place. ✔ Ensure that the flow rate of the pump is constant and check that there are no leaks in the chromatographic system. Agilent 1260 Infinity ELSD User Manual...

  • Page 68: If There Is No Response From The System

    Pry the plastic cover off the fuse holder to access the fuses and inspect them. If a fuse(s) is blown, replace it with one of the same rating (T3.15 AL/230V, part number G4218-68005 Fuses Figure 16 Control Panel Agilent 1260 Infinity ELSD User Manual...

  • Page 69: Perform The Noise Tests

    77) fails or is significantly different from values obtained for comparable measurement conditions (same system setup, solvent column), it is likely that the problem is due to the column (e.g. highly retained compounds are being eluted). Agilent 1260 Infinity ELSD User Manual...

  • Page 70: Specific Detector Troubleshooting

    In this situation, increase the temperature to 90 C and pump solvent at the rate of 2 ml/min, using a gas pressure of 3.5 bar (51 psi). Agilent 1260 Infinity ELSD User Manual...

  • Page 71: Noise Test Procedures

    The solvent noise tests measures the signal created by pure solvent flowing through the detector while no column is installed ✔ The column noise test measures the signal created by pure solvent flowing through the column and the detector. Agilent 1260 Infinity ELSD User Manual...

  • Page 72: Preliminary Activities

    Replacement filter cartridges are available as part number G4218-40150. 5 Press the button. The signal should read close to zero and remain constant. 6 Set the noise filtering to 1S (see “The Noise Filter/Pressure Unit Screen” page 101). Agilent 1260 Infinity ELSD User Manual...

  • Page 73: Electronic Noise Test

    5 At gain 12, monitor the signal for a period of 5 min. The variation in the signal should be less than +/- 2 mV (there may be some spiking of the signal). 6 Record the level and autozero the detector again. Agilent 1260 Infinity ELSD User Manual...

  • Page 74: Background Noise (Stray Light) Test

    6 Set the offset to 0 mV. 7 Wait 15 minutes for stabilization and record the signal level. 8 Increase the gain to 12 and monitor the signal. The expected level is typically 100 mV to 150 mV. Agilent 1260 Infinity ELSD User Manual...

  • Page 75: Solvent Noise Test

    Residue after Evaporation; this parameter should be less than 1 ppm to maximize the sensitivity of the detector. Mass spectroscopy grade solvents have a very low dry residue. Agilent 1260 Infinity ELSD User Manual...
  • Page 76 If changing the solvent source does not solve the problem, it may be necessary to decontaminate the system as described in “General Inspection” on page 82 or clean the nebulizer as described in “Cleaning the Detector” on page 83. Agilent 1260 Infinity ELSD User Manual...

  • Page 77: Column Noise Test

    HPLC system for 12 h before starting to analyze unknown samples. This wash should be performed after the column noise test is completed, but does not need not be performed after each analysis. Agilent 1260 Infinity ELSD User Manual...

  • Page 78: Troubleshooting Based On Diagnostic Tests

    (see “Cleaning the Nebulizer” on page 86), make sure you are using a fresh sample and consider running the test using a backpressure loop instead of a column. Agilent 1260 Infinity ELSD User Manual...

  • Page 79: Troubleshooting Based On Instrument Signals

    If the stray light test passes and the gas flow is on, the nebulizer is probably blocked. Clean the nebulizer as described in “Cleaning the Nebulizer” page 86. ✔ If cleaning the nebulizer does not solve the problem, replace the nebulizer. Agilent 1260 Infinity ELSD User Manual...

  • Page 80: Decrease In Sensitivity

    Then: 1 Do a general inspection as described in “General Inspection” on page 82 2 Clean the nebulizer as described in “Cleaning the Nebulizer” on page 86. 3 Exchange the nebulizer. Agilent 1260 Infinity ELSD User Manual...

  • Page 81: Maintenance

    Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual Maintenance General Inspection Cleaning and Decontamination Adjusting Stray Light Settings This chapter describes: • The maintenance procedures that should be performed by the operator on a routine basis (see “General Inspection”...

  • Page 82: General Inspection

    Maintenance General Inspection General Inspection The Agilent 1260 Infinity Evaporative Light Scattering Detector is designed for a low level of maintenance activities. Maintenance activities are normally the responsibilities of the user. If preventive maintenance activities are followed, the system provides high sensitivity measurements without intervention of the operator.

  • Page 83: Cleaning And Decontamination

    They could cause toxications and other health risks. => If you have any doubt about the cleanliness of a detector, treat the detector as if it contained a potentially harmful substance and decontaminate it before working on it. Agilent 1260 Infinity ELSD User Manual...

  • Page 84: Removing The Nebulization Chamber And Nebulizer

    “Powering Down and Shutting Down the System” on page 56. 1 Turn off the LC pump or any other mobile phase flow. 2 Turn off the ELS detector. 3 Stop the gas flow using the external pressure regulator. Agilent 1260 Infinity ELSD User Manual...
  • Page 85 For subsequent steps, see Figure 4 Remove the capillary connection to the mobile phase 5 Remove the connection to pressurized gas by pushing on the white inlet (see arrow). 6 Remove the drain tube. Agilent 1260 Infinity ELSD User Manual...

  • Page 86: Cleaning The Nebulizer

    To remove the nebulizer from the system: 1 Turn off the HPLC system. 2 Disconnect the nebulizer liquid inlet from the column. 3 Disconnect the gas inlet from the nebulizer. Agilent 1260 Infinity ELSD User Manual...
  • Page 87 In most cases, ethanol is a satisfactory solvent. 2 Place the nebulizer vertically in the solvent bath. The nebulizer outlet should be placed at the bottom. Agilent 1260 Infinity ELSD User Manual...
  • Page 88 2 Fix the nebulizer on the nebulization chamber. 3 Test the nebulizer to ensure that it is working properly. If the performance cannot be improved by cleaning the nebulizer, a new nebulizer might be required. Agilent 1260 Infinity ELSD User Manual...

  • Page 89: Adjusting Stray Light Settings

    Maintenance Adjusting Stray Light Settings Adjusting Stray Light Settings You may set the desired stray light using the instrument keyboard. Agilent 1260 Infinity ELSD User Manual...
  • Page 90 Maintenance Adjusting Stray Light Settings Agilent 1260 Infinity ELSD User Manual...

  • Page 91: Parts For Repair

    Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual Parts for Repair Evaporative Light Scattering Detector Parts Agilent Technologies...

  • Page 92: Evaporative Light Scattering Detector Parts

    Parts for Repair Evaporative Light Scattering Detector Parts Evaporative Light Scattering Detector Parts Figure 18 Evaporative Light Scattering Detector Parts Agilent 1260 Infinity ELSD User Manual...
  • Page 93 G4218-40140 Bulkhead Fitting [8] G4218-40130 Front panel shield window G4218-40400 Autozero Cable G4218-81101 Signal Cable G4218-81100 RS 232 Cable G4218-81103 External Event Cable G4218-81102 Cable Remote 5061-3378 Caffeine Standard 250 µg/ml in water G4218-85000 Agilent 1260 Infinity ELSD User Manual...
  • Page 94 0.2 ml/min – 0.4 ml/min – White Seal G4218-20004 Nebulizer 1.4 ml/min 1.0 ml/min 1 Ring * The optimum flow range provides highest sensitivity and repeatability † 14 bar at 1.4 ml/min with water/acetonitrile, 50:50) Agilent 1260 Infinity ELSD User Manual...

  • Page 95: The Control Panel

    Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual The Control Panel The Control Panel This chapter describes the role of the controls and the digital display on the control panel Agilent Technologies...

  • Page 96: The Control Panel

    The Control Panel The Control Panel The Control Panel The Control Panel (Figure 19) includes a digital display and a number of buttons that are used to enter data. Figure 19 The Control Panel Agilent 1260 Infinity ELSD User Manual...

  • Page 97: The Digital Display

    (e.g. the offset) by 1 unit. If you keep the key pressed, the rate of change of the parameter will increase. sets the value of the parameter that you have edited. Agilent 1260 Infinity ELSD User Manual...

  • Page 98: The User Interface

    22) and the pressure units can be selected via the Noise Filter/Pressure Unit screen (Figure 24). The temperature value blinks if desired temperature is not reached and stable. The pressure value blinks if the gas pressure is lower than 3.0 bar. Agilent 1260 Infinity ELSD User Manual...
  • Page 99 A wait time of 15 to 30 minutes is recommended for achieving good temperature stabilization.. The stabilization time for detector temperatures close to the ambient temperature is longer than for high temperatures. Agilent 1260 Infinity ELSD User Manual...
  • Page 100 This setting can be changed with the buttons and validated by the button. After you have set the desired autozero offset, press the button for navigating to the Noise Filter/Pressure Unit screen (Figure 24). Agilent 1260 Infinity ELSD User Manual...
  • Page 101 LED screen When you press the (Figure 25) will be shown. Agilent 1260 Infinity ELSD User Manual...
  • Page 102 Value : 100 % Figure 26 The Light Source Normalization Screen The intensity of the light source and consequently the measured signals will decrease over time. After replacing the light source, the signal intensity may also change. Agilent 1260 Infinity ELSD User Manual...
  • Page 103 28) is used to indicate which features should be shut down upon receipt of a power down signal from an external source (e.g. a personal computer or an HPLC pump) or from the menu. Agilent 1260 Infinity ELSD User Manual...
  • Page 104 To leave the power down mode, release the contact closure if power down has been N O T E activated by external event or press any key if power down has been activated from the Power down screen. Agilent 1260 Infinity ELSD User Manual...
  • Page 105 31) will be presented. The Serial Number Screen Serial Number 0380001A Figure 31 The Serial Number Screen The Serial Number screen cannot be edited. When you press the button, the Firmware screen (Figure 32) will be presented. Agilent 1260 Infinity ELSD User Manual...
  • Page 106 The Factory Method Code Screen Factory Method Code ______ Authorized persons only Figure 33 The Factory Method Code Screen The Factory Method Code screen is used by the service engineer to access procedures required for the instrument service. Agilent 1260 Infinity ELSD User Manual...

  • Page 107: Appendix

    Agilent 1260 Infinity Evaporative Light Scattering Detector User Manual Appendix Safety Information Solvent Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Lithium Batteries Information Agilent Technologies on Internet Agilent Technologies...

  • Page 108: Safety Information

    The Agilent 1260 Infinity modules are designed and certified as a general purpose laboratory instrument for research and routine application only. It is not certified for in-vitro or medical applications.
  • Page 109 The gas pressure should not exceed 4.5 bar (67 psi). Make sure that the gas flow is maintained while the mobile phase flows through the system. If the gas flow is interrupted for extended periods of time, organic solvents could possibly damage the pressure sensor and/or the photosensor. Agilent 1260 Infinity ELSD User Manual...
  • Page 110 Electric shock risk The apparatus is marked with this symbol when the user should refer to the instruction manual in order to prevent risk of harm to the operator and to protect the apparatus against damage. Agilent 1260 Infinity ELSD User Manual...
  • Page 111 A caution alerts you to situations that could cause a possible loss of data. C A U T I O N ⇒ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 1260 Infinity ELSD User Manual...

  • Page 112: Solvent Information

    For example, a 1-% solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. Agilent 1260 Infinity ELSD User Manual...

  • Page 113: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)

    Annex I, this product is classed as a “Monitoring and Control instrumentation” product. Do not dispose off in domestic household waste! To return unwanted products, contact your local Agilent office, or www.agilent.com for more information. Agilent 1260 Infinity ELSD User Manual...

  • Page 114: Lithium Batteries Information

    WA R N I N G av apparatfabrikanten. Brukt batteri returneres appararleverandoren. Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien N O T E maar inleveren als KCA. Agilent 1260 Infinity ELSD User Manual...

  • Page 115: Agilent Technologies On Internet

    For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products > Chemical Analysis It will provide also the latest firmware of the Agilent 1260 Infinity modules for download. Agilent 1260 Infinity ELSD User Manual...

  • Page 116: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent 1260 Infinity ELSD User Manual...

  • Page 117: Index

    Factory Method Code nebulizer environmental conditions Firmware cleaning evaporation of the solvent Gas Valve exhaust requirements installation external auto-zero Noise Filter/Pressure Unit Noise Filter/Pressure Unit Offset noise tests Serial Number Agilent 1260 Infinity ELSD User Manual...
  • Page 118 Status Temperature/Gain Total Lifetime Elapsed Serial Number screen shutting down siphon overflow (installing) solvent noise solvent noise test start-up procedure Status screen stray light test temperature (optimizing) Temperature/Gain Screen Temperature/Gain screen tests background (stray light) noise Total Lifetime Elapsed screen troubleshooting unpacking user interface...
  • Page 120 • Installation of the Detector • Start-Up Procedure • Operating the System • Maintenance and Troubleshooting • Specifications • Spare Parts List • Electrical connections • Safety Information © Agilent Technologies 2008, 2009, 2010 Printed in Germany 06/2010 *G4218-90002* *G4218-90002* G4218-90002 Agilent Technologies...

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