Optimization Overview - Agilent Technologies 1260 User Manual

Optimization Overview

Table 27

Optimization Overview

Parameter
1 Selection of flow cell
• Choose flow cell according to used column, see
2 Connection of flow cell
• For flow rates from 0.5 ml/min connect column using the zero-dead-volume
fittings of the detector.
• For small column i.d. (e.g 1 mm) the inlet capillary of the micro flow cell can
be connected directly to the column.
3 Setting the peak width (response time)
• Use peak width according
• Set the peak-width close to the width of a narrow peak of interest in your
chromatogram.
4 Setting wavelength and bandwidth
• Sample wavelength:
• Never miss a peak by the use of a browser wavelength like 250 nm with
100 nm bandwidth.
• Select specific wavelength with reduced bandwidth if you need
selectivity, e.g. 250,10 nm and 360,100 nm as reference wavelength.
• Set the sample wavelength to a peak or valley in the spectrum to get
best linearity for high concentrations.
• Reference wavelength:
• Select the reference wavelength with broad bandwidth (30...100 nm)
wavelength range where your analytes have little or no absorbance (e.g.
sample at 254 nm, reference at 320 nm).
Agilent 1260 Infinity DAD and MWD User Manual
Figure 50
Figure 50 on page 118 as starting point.
How to optimize the Detector
Impact
• peak resolution versus sensitivity
on page 118.
• chromatographic resolution
• peak resolution versus sensitivity
versus disk space
• sensitivity versus selectivity
• sensitivity versus linearity
• baseline drift due to RI effects.
Optimization Overview
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