Agilent Technologies 1260 User Manual page 120

6
How to optimize the Detector
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion
Figure 51
Traditionally LC analysis with UV detectors is based on comparing
measurements with internal or external standards. To check photometric
accuracy of the detector it is necessary to have more precise information on
path lengths of the flow cells.
The correct response is:
expected response * correction factor
Please find below the details of the flow cells:
Table 28 Correction factors for flow cells
Flow cell
Standard flow cell, 10 mm, 13 µL, 120 bar (12 MPa) (p/n
G1315-60022)
Semi-micro flow cell, 6 mm, 5 µL, 120 bar (12 MPa) (p/n
G1315-60025)
Micro flow cell, 3 mm, 2 µL, 120 bar (12 MPa) (p/n
G1315-60024)
Semi-nano flow cell kit, 10 mm, 500 nL, 5 MPa (p/n
G1315-68724)
Nano flow cell kit, 10 mm, 80 nL, 5 MPa (p/n G1315-68716)
120
Influence of Cell Path Length on Signal Height
Path length (actual) Correction factor
9.80 ±0.07 mm 10/9.8
5.80 ±0.07 mm 6/5.8
3.00 +0.05 mm/-0.07 3/3
mm
10.00 ±0.02 mm 10/10
6.00 ±0.02 mm 6/6
Agilent 1260 Infinity DAD and MWD User Manual