Agilent Technologies 1260 User Manual page 118

6
How to optimize the Detector
Optimization Overview
Table 27 Optimization Overview
Parameter
5 Setting the slit width
• Use 4 nm slit for normal applications.
• Use narrow slit (e.g 1 nm) if your analytes have narrow absorbance bands
and for high concentrations.
• Use a wide slit (e.g. 16 nm) to detect very low concentrations.
• Optimizing spectral acquisition (DAD only)
• Select spectra acquisition mode according to your needs (see
page 109).
• Set the spectral wavelength range (for colorless samples 190...400 nm is
sufficient).
• Set step to 4 nm for normal use; set small step (and slit width) if high
resolution of spectra with fine structure is wanted.
Figure 50
118
Choosing a Flow Cell in HPLC
Impact
• spectral resolution, sensitivity and
linearity.
Table 25 on
Agilent 1260 Infinity DAD and MWD User Manual