Agilent Technologies 1260 User Manual page 85

Table 20 Choice of Priming Solvents for Different Purposes
Activity
After an installation
When switching between reverse
phase and normal phase (both times)
After an installation
Cleaning the system when using
buffers
After a solvent change
After the installation of normal phase
seals (P/N 0905-1420)
The pump should never be used for priming empty tubings (never let the pump run dry). Use
N O T E
a syringe to draw enough solvent to completely fill the tubings up to the pump inlet before
you continue priming with the pump.
1 Open the purge valve of your pump (by turning it counterclockwise) and set
2 Flush all tubes with at least 30 ml of solvent.
3 Set flow to required value of your application and close the purge valve.
Agilent 1260 Infinity DAD and MWD User Manual
Solvent
Isopropanol
Isopropanol
Ethanol or Methanol
Bidistilled water
Bidistilled water
Hexane + 5% Isopropanol
flow rate to 3-5 ml/min.
Pump for approximately 10 minutes before starting your application.
Using the Detector
Setting up an Analysis
Comments
Best solvent to flush air out of the
system
Best solvent to flush air out of the
system
Alternative to Isopropanol (second
choice) if no Isopropanol is available
Best solvent to re-dissolve buffer
crystals
Best solvent to re-dissolve buffer
crystals
Good wetting properties
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