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•
Insufficient time for system equilibration after turning on lamp(s)
To achieve optimum results, allow 60 minutes for the lamp(s) to stabilize
before beginning operation.
•
Leaking flow cell
Tighten fittings. Also, check that the backpressure on the cell does not exceed
the specification (see
If the cell continues to leak, replace it (see
•
Lamp needs replacement
The light output of the lamp decreases over time. The lower light output may
increase noise above an acceptable level or cause baseline drift, especially if
the lamp has been in service for more than 2000 hours. If this occurs, replace
the lamp. For deuterium lamp (P/N 066347) installation instructions, see
Section
5.3. For tungsten lamp (P/N 066348) installation instructions, see
Section
5.4.
4.7
Peak Broadening
•
Tubing inner diameter too large
Refer to
Section 5.1
tubing. Replace tubing if needed.
•
Mobile phase or post-column reagent is degraded
Prepare all mobile phases and reagents with spectro-grade solvents, reagent-
grade chemicals, and ASTM Type I (18 megohm-cm) filtered, deionized
water that meets the specifications listed in
•
System-level problem
1. Check the system for blocked tubing.
2. The column may be overloaded. Reduce the sample size or dilute the
sample.
3. The column may be contaminated. Clean or replace the column.
4. The end-line filters on the mobile phase lines are clogged. Replace the
filters.
Doc. 065377-01 1/12
Section
A.5).
for tubing requirements for the cell inlet and outlet
4 • Troubleshooting
Section
5.6).
Table
B-1.
59
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