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Dionex VWD Operator's Manual
Gardez à l'esprit que les circuits hydrauliques du détecteur peuvent
être remplis de solvants toxiques. Par conséquent, purgez le
détecteur avec un solvant approprié et portez des vêtements de
protection avant de commencer les opérations de maintenance ou de
réparation.
Bedenken Sie, dass die fluidischen Bauteile des Detektors eventuell
mit giftigen Lösungsmitteln gefüllt sind. Spülen Sie daher vor Beginn
der
Lösungsmittel und tragen Sie Schutzkleidung
5.2
Wavelength Calibration and Verification
Wavelength calibration is performed automatically at the following times:
•
When a detector lamp is turned on for the first time after the detector power is
turned on.
•
After the detector resumes operation from standby mode.
Calibration starts shortly after the lamp is ignited. The 0-order position of the
grating is established; this is the point where the grating is positioned to reflect all
light from the lamps without diffraction. It is used as the "home" position for the
grating.
In addition, the wavelength accuracy can be verified via the holmium oxide filter
installed in the beam path of the lamp. The maxima are determined from the
resulting transmission spectrum and compared to the holmium oxide values stored
in the detector firmware.
At a minimum, wavelength verification should be performed after changing the
flow cell. Perform wavelength verification more often if your application is very
sensitive to wavelength variations.
Before verifying wavelengths, observe the following precautions:
•
Allow enough time (typically 15 minutes) to ensure that each lamp has
reached the operating temperature. A lamp's spectrum changes significantly
during the first few minutes after the lamp is turned on.
•
Make sure the baseline is stable. The baseline may become unstable if the
solvent composition has been modified, or if air bubbles exist in the light
path.
64
Wartungsarbeiten
den
Detektor
mit
einem
geeigneten
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