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Thermo Scientific Dionex Aquion RFIC Operator's Manual

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Thermo Scientific
Dionex Aquion RFIC Ion
Chromatography System
Operator's Manual
22176-97006 Revision 01 March 2019

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  • Page 1 Thermo Scientific Dionex Aquion RFIC Ion Chromatography System Operator’s Manual 22176-97006 Revision 01 March 2019...
  • Page 2 © 2019 Thermo Fisher Scientific Inc. All rights reserved. Chromeleon, ERS, and OnGuard are registered trademarks of Thermo Fisher Scientific Inc. in the United States. Microsoft and Windows are registered trademarks of Microsoft Corporation in the United States and other countries. KIMWIPES is a registered trademark of Kimberly-Clark Corporation in the United States and possibly other countries.

  • Page 3: Table Of Contents

    Introduction to Ion Chromatography (IC) ......1 Overview of the Dionex Aquion RFIC ....... . 3 Chapter 2 Description .
  • Page 4 Troubleshooting Error Messages ........51 Dionex Aquion RFIC Operator’s Manual...
  • Page 5 Controlling TTL and Relay Outputs....... . 135 Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 6 Index ............. . .151 Dionex Aquion RFIC Operator’s Manual...

  • Page 7: Preface

    In addition to this manual, the following documents are available on the Thermo Fisher Scientific website or from your local office: • Dionex Aquion RFIC Ion Chromatography System Installation Instructions (Document No. 22176-97005) • Manuals for consumable products (including columns, suppressors, and eluent generator cartridges) •...

  • Page 8: Safety Information

    The Dionex Aquion RFIC is designed for IC (ion chromatography) applications and should not be used for any other purpose. Operation of a Dionex Aquion RFIC in a manner not specified by Thermo Fisher Scientific may result in personal injury.

  • Page 9: Safety Symbols

    • CAN/CSA-C22.2 No. 61010-1-12 • EN 61326-1:2013 The CE mark on the Dionex Aquion RFIC model/data label indicates that the system is in compliance with the following European Community Directives as is evidenced by compliance to the associated standard where appropriate: •...

  • Page 10: Notice On Lifting And Handling Of Thermo Scientific Instruments

    • WEEE product class • Name of the manufacturer or distributor (where you purchased the product) • Number of product pieces, and the estimated total weight and volume viii Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 11 Richtlinie 2002/96/EC. Es ist mit dem folgenden Symbol gekennzeichnet: Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Weitere Informationen finden Sie unter www.thermoscientific.de/rohs. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 12: Deionized Water Requirements For Ic

    For Technical Support for Dionex products  In the U.S. and Canada, call 1-800-532-4752. Outside the U.S. and Canada, call the nearest Thermo Fisher Scientific office. For additional contact information  Go to www.thermofisher.com/us/en/home/technical-resources/contact-us.html. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 17: Chapter 1 Introduction

    Introduction Introduction to Ion Chromatography (IC) The Dionex Aquion RFIC performs ion analyses using suppressed or non-suppressed conductivity detection. An ion chromatography system typically consists of a liquid eluent, a high-pressure pump, a sample injector, a guard and separator column, a chemical suppressor, a conductivity cell, and a data collection system.
  • Page 18 6. Data Analysis • The conductivity cell transmits the signal to a data collection system. • The data collection system (for the Dionex Aquion RFIC, this is the Chromeleon 7 Chromatography Data System) identifies the ions based on retention time, and quantifies each analyte by integrating the peak area or peak height.

  • Page 19: Overview Of The Dionex Aquion Rfic

    If necessary, the Dionex Aquion RFIC can be configured with a column heater for temperature control of the column. Dionex Aquion RFIC operation is controlled remotely by a personal computer running the Microsoft®...
  • Page 20 Introduction Overview of the Dionex Aquion RFIC Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 21: Chapter 2 Description

    Description This chapter describes key features of the Dionex Aquion RFIC and introduces the Chromeleon user interface. Contents • Operating Features • Flow Schematics • Chromeleon Chromatography Data System • System Component Details Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 22: Operating Features

    For more information about sample injection, see “Operation Overview” page LEDs The Power LED indicates whether the Dionex Aquion RFIC power is on. Other LEDs indicate the status of various system functions. For details, see Table Dionex Aquion RFIC Operator’s Manual...
  • Page 23 Description Operating Features Figure 3. Dionex Aquion RFIC front panel LEDs Table 2. Dionex Aquion RFIC front panel LED states LED label If on (green) If flashing Load Injection valve is in Valve error detected Load position Inject Injection valve is in...

  • Page 24: Top Cover

    – The tubing chase under the connectors routes tubing from the eluent reservoir and Dionex EGC holder to the front of the Dionex Aquion RFIC. • Dionex EGC service area – The service area holds the Dionex EGC during installation and replacement.

  • Page 25: Component Panel

    Description Operating Features Component Panel Figure 5 shows the user-accessible components installed on the component panel behind the Dionex Aquion RFIC front door. Figure 5. Dionex Aquion RFIC component panel Tubing chase Mounting brackets for second suppressor Column heater (optional)
  • Page 26 Operating Features Pump Heads The Dionex Aquion RFIC includes a dual-piston serial pump. The flow rate can be set to 0.00 mL/min or to between 0.05 and 5.00 mL/min. For optimum performance, set the flow rate to between 0.40 and 2.00 mL/min. Setting the flow rate to 0.00 mL/min turns off the pump.

  • Page 27: Rear Panel

    Rear Panel Figure 6 shows the Dionex Aquion RFIC rear panel. Figure 6. Dionex Aquion RFIC rear panel Tubing clips Power switch...
  • Page 28 Operating Features Analog Output Connector The analog output connector outputs conductivity data (as a 0 to 1 V signal) to an integrator or recording device. For connection and setup information, refer to the Dionex Aquion RFIC installation instructions. USB Connectors The USB receptacle provides a connection to the Chromeleon computer.

  • Page 29: Flow Schematics

    The required components include a Dionex EGC 500 Eluent Generator Cartridge and aThermo Scientific™ Dionex™ EGC 500 Carbonate Mixer. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 30 Description Flow Schematics Figure 7. Dionex Aquion RFIC flow schematic: KOH, LiOH, NaOH, or MSA eluent generation Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 31: Generation

    • Regenerant waste from the suppressor is directed back to the Dionex CR-TC , and then to the Dionex EGC degas tubing , where any released hydrogen or oxygen gas is removed before it is sent to the gas separator assembly and then to waste Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 32 Description Flow Schematics Figure 8. Dionex Aquion RFIC flow schematic: carbonate eluent generation Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 33: Flow Description For Carbonate Eluent Generation

    • Regenerant waste from the suppressor is directed back to the Dionex EGC degas tubing , where any released hydrogen or oxygen gas is removed before it is sent to the gas separator assembly and then to waste Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 34: Chromeleon Chromatography Data System

    Chromeleon Chromatography Data System Chromeleon Chromatography Data System Chromeleon software is used to control the Dionex Aquion RFIC and to acquire and process data. There are two modes of software control: • With automated control, you create a list of control commands to be executed in chronological order.

  • Page 35: System Component Details

    Vacuum Degas Assembly (Optional) The vacuum degas assembly provides online eluent degassing at a user-specified time and duration. The assembly, which must be installed in the Dionex Aquion RFIC at the factory, consists of: • A single-channel degas chamber (with degas membranes) with internal capacity of 17 mL •...
  • Page 36 To select the degas operating mode  By default, the Dionex Aquion RFIC monitors the degas pressure reading and turns the degas pump on and off as required. (This is the Monitor mode.) Follow the instructions here to select a different operating mode.

  • Page 37: Eluent Valve

    Figure 12. Eluent valve Pump The Dionex Aquion RFIC pump is a microprocessor-based eluent delivery system. Its variable speed, dual-piston series design ensures pulse-free pumping for the most demanding applications. Primary Pump Head...
  • Page 38 Flow output from the pressure transducer continues to the pulse damper, which smooths minor pressure variations. From there, flow is directed to the injection valve and then to the remainder of the chromatography system. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 39: Eluent Generator

    Note The waste, gas separator tube (P/N 045460) should be connected to the system waste line during installation. For more information, refer to the Dionex Aquion RFIC installation instructions. Several eluent generator cartridge types are available for use with the Dionex Aquion RFIC (see Table 3).
  • Page 40 4). The cartridge holder also houses a high-pressure degas tubing assembly. Tubing and fittings for plumbing the cartridge, degas assembly, and Dionex CR-TC are included with the holder. For more information, refer to the Dionex Aquion RFIC installation instructions. Backpressure Coil (Optional) The Dionex EGC requires at least 14 MPa (2000 psi) of system backpressure for removal of electrolysis gas from the eluent produced by the cartridge.

  • Page 41: Injection Valve

    The valve has two operating positions: Load and Inject (see Figure 14). Figure 14. Injection valve flow schematics LOAD POSITION INJECT POSITION Sample In Sample In To Column To Column Sample Sample Loop Loop To Waste From Pump To Waste From Pump Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 42: Auxiliary Valve (Optional)

    • Matrix elimination (for example, the removal of high backgrounds of chloride or organic material) • Concentrator-based techniques • Conditional injections (large loop/small loop applications where the data system monitors sample concentration and reinjects the sample, using the smaller loop, if the concentration is too high) Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 43: Column Heater (Optional)

    65 °C, the column heater is shut off and an error message is displayed in the Chromeleon audit trail. For troubleshooting guidance, see “Column heater exceeds safe temperature” page The column heater can be installed at the factory or installed on-site by Thermo Fisher Scientific field service personnel. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 44: Suppressor

    Table 5 lists the suppressors that can be used with the Dionex Aquion RFIC. For details about these suppressors (or guidelines for selecting a suppressor for an application), refer to the suppressor manuals. Table 5. Thermo Scientific suppressors for the Dionex Aquion RFIC...
  • Page 45 Figure 5). The remaining cell components are mounted behind the component panel. The cell must be installed at the factory or ordered separately and installed on-site by Thermo Fisher Scientific field service personnel. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 46 Description System Component Details Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 47: Operation

    Note The instructions in this chapter assume that the initial setup of the Dionex Aquion RFIC (including configuring the system in a Chromeleon instrument) has been completed. If this is not the case, set up the system before proceeding. For more information, refer to the Dionex Aquion RFIC installation instructions. Contents •...

  • Page 48: Operation Overview

    Operation Operation Overview Operation Overview Figure 17 shows the basic steps for routine operation of the Dionex Aquion RFIC. Figure 17. System operation overview Power up the system Start Chromeleon Set up the reservoir (or RFIC-ER system) Check all connections...

  • Page 49: Sample Processing Overview

    Samples can be run manually (one at a time) or else grouped and run automatically in batches. Figure 18 shows the typical steps for each type of sample processing. Figure 18. Sample processing overview Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 50: Turning On The System Power

    Turning On the System Power To turn on the power  1. Press the main power switch on the Dionex Aquion RFIC rear panel (see Figure 19). 2. Turn on the power to the computer and the autosampler (if installed).

  • Page 51: Connecting To Chromeleon

    To display the ePanel Set  1. In the Console, click the Instruments Category Bar. 2. Select the name of the instrument in which the Dionex Aquion RFIC is configured. Chromeleon will connect to the instrument and display the ePanel Set (see Figure 20).

  • Page 52: Setting Up The Eluent Reservoir

    Filtering removes small particulates in the deionized water that may contaminate the pump check valves and cause erratic flow rates or loss of prime. 1. Locate the end-line filter (P/N 045987) provided in the Dionex Aquion RFIC Ship Kit (P/N 064375).

  • Page 53: Checking All Connections

    90) before starting this pump priming procedure. To prime the pump  1. Verify that the priming valve on the primary pump head is closed (turned all the way clockwise) (see Figure 22). Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 54: Setting System Operating Conditions

    1. Verify that the pump is on and that the flow rate setting is correct. 2. Verify that the suppressor current is on and that the setting is correct. 3. Verify that the Dionex EGC is on and that the eluent concentration setting is correct. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 55: Equilibrating The System And Verifying Operational Status

    • If the conductivity is too high, see “High Cell Output” page 66 troubleshooting guidance. • If there is baseline drift or excessive baseline “noise” (large fluctuations in readings), see “Baseline Noise or Drift” page 67 for troubleshooting guidance. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 56: Preparing Samples

    Before injection, pretreat samples that may contain high concentrations of interfering substances by putting them through Thermo Scientific™ Dionex™ OnGuard™ cartridges. For instructions, refer to the OnGuard cartridge manual. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 57: Diluting Samples

    Loading and Injecting Samples There are two techniques for loading samples into the sample loop: • With an autosampler • With a syringe or vacuum syringe via the injection port on the Dionex Aquion RFIC front door (see Figure For autosampler injections, the injection port tubing must be disconnected from the Dionex Aquion RFIC injection valve and replaced by the autosampler outlet tubing.
  • Page 58 2. Fill the 1-cc syringe (P/N 016388) provided in the Dionex Aquion RFIC Ship Kit (P/N 064375) with a calibration standard or sample. 3. Insert the syringe into the injection port on the Dionex Aquion RFIC front door (see Figure 4.

  • Page 59: Processing Samples

    “Equilibrating the System and Verifying Operational Status” page 39 to prepare the Dionex Aquion RFIC for operation and to prepare the sample for processing. 2. Load the sample, using a syringe, vacuum syringe, or autosampler (see“Loading and Injecting Samples”...

  • Page 60: Automated (Batch) Sample Processing

    1. Complete the instructions in “Turning On the System Power” page 34 through “Equilibrating the System and Verifying Operational Status” page 39 to prepare the Dionex Aquion RFIC for operation and to prepare the sample for processing. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 61 For detailed instructions, refer to the autosampler manual. If an autosampler is not installed: Load the sample into the injection valve sample loop through the sample port on the Dionex Aquion RFIC front door (see “To load samples with a syringe”...
  • Page 62 Operation Processing Samples Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 63: Chapter 4 Maintenance

    Maintenance This chapter describes routine maintenance procedures for the Dionex Aquion RFIC that users can perform. All other maintenance procedures must be performed by Thermo Fisher Scientific personnel. Daily Maintenance • Check the Dionex Aquion RFIC component panel (see Figure 5) for leaks or spills.

  • Page 64: Semiannual Maintenance

    • Thermo Fisher Scientific recommends performing preventive maintenance of the Dionex Aquion RFIC annually, as well as before any scheduled Performance Qualification tests. The Dionex Aquion RFIC Preventive Maintenance Kit (P/N 057954) includes replacement parts and instructions. • Rebuild the auxiliary valve, if installed (see “Rebuilding the Injection Valve or Auxiliary...

  • Page 65: Troubleshooting

    Set and select Pump_ECD Device Troubleshooting. Error Messages The instrument control firmware installed in the Dionex Aquion RFIC periodically checks the status of certain parameters. If a problem is detected, it is reported to Chromeleon and logged in the software audit trail. Each error message is preceded by an icon that identifies the...
  • Page 66 A message is displayed in the audit trail and the running queue is aborted. Table 8 lists the most frequently observed Dionex Aquion RFIC error message and their default severity levels. For troubleshooting guidance, refer to the page indicated in the table. Table 8. Chromeleon audit trail error messages...

  • Page 67: Troubleshooting Error Messages

    Column heater exceeds safe temperature This error occurs when the column heater temperature is higher than the maximum temperature allowed. This may occur, for example, if the Dionex Aquion RFIC is operating in an environment in which the temperature exceeds 40 C (104 °...
  • Page 68 Dionex CR-TC is turned off automatically to prevent it from being damaged. EGC board not present This error occurs when the Dionex Aquion RFIC receives a Dionex EGC-related command from Chromeleon but is unable to recognize the Dionex EGC controller board.
  • Page 69 2. Run the calibration again. EGC disconnected error This error occurs if the Dionex EGC is disconnected from the Dionex Aquion RFIC at the following times: • When Chromeleon is attempting to send a Dionex EGC-related command to the system.
  • Page 70 2. If the error message appears again, it may indicate a malfunction in the Dionex EGC control electronics. Contact Technical Support for Dionex products for assistance. Note Dionex Aquion RFIC electronics components cannot be serviced by the user. EGC over voltage This error occurs when the voltage applied to the Dionex EGC exceeds the maximum allowable voltage.
  • Page 71 2. Try to toggle the valve from LoadPosition to InjectPosition by clicking the down arrow next to Inject on the Home ePanel. 3. Turn off the Dionex Aquion RFIC power briefly, and then restart the system. 4. If the problem persists, repeat Step 5.
  • Page 72  Contact Technical Support for Dionex products for assistance. Note Dionex Aquion RFIC electronics components cannot be serviced by the user. Pump over pressure If the system pressure exceeds the set limit for at least 0.5 second, this error occurs and the pump stops.
  • Page 73 Chromeleon. To troubleshoot  Verify that the USB cable is connected correctly from the Dionex Aquion RFIC rear panel to the computer on which Chromeleon is installed. For details, refer to the Dionex Aquion RFIC installation instructions. Pump under pressure If the system pressure falls below the low pressure limit, this error occurs and the pump stops.
  • Page 74 Troubleshooting Troubleshooting Error Messages Suppressor not connected This error occurs if you turn on the suppressor but the Dionex Aquion RFIC cannot establish a connection with it. To troubleshoot  1. Check the suppressor cable connection (see “Replacing the Suppressor”...

  • Page 75: Troubleshooting System Component Symptoms

    In this situation, the suppressor is turned off automatically to prevent it from being damaged. Troubleshooting System Component Symptoms Table 9 lists symptoms related to problems with system components that may occur during operation of the Dionex Aquion RFIC. Table 9. System component symptoms Symptom category Symptom Leaks...

  • Page 76: Liquid Leaks

    Installation of Dionex Liquid Line Fittings (Document No. 031432). 2. If the leak persists, contact Technical Support for Dionex products for assistance. Note Dionex Aquion RFIC electronics components cannot be serviced by the user. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 77 2. Verify that the plumbing downstream from the cell is clear; a blockage may overpressurize the cell, causing a leak. 3. If the leak persists, contact Technical Support for Dionex products for assistance. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 78: Pump Difficult To Prime Or Loses Prime

    Liquid leaks at junction 1. Using the open-end wrench (P/N 014605) provided in between pump head and the Dionex Aquion RFIC Ship Kit (P/N 064375), tighten pump casting the two acorn nuts that attach the pump head to the pump housing (see Figure 28).

  • Page 79: Pump Does Not Start

    Procedure)” page 90). Broken pump piston Replace the piston (see “Replacing a Pump Piston” page 83). Eluent generator degas Replace the Dionex EGC (see “Replacing the Dionex EGC” tubing assembly ruptured page 93). Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 80: Erratic Flow And/Or Pressure Reading

    Clean the columns as instructed in the column manual or replace the guard column. Plugged injection valve or Rebuild the valve (see “Rebuilding the Injection Valve or auxiliary valve passages Auxiliary Valve” page 74). Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 81: Peak Ghosting

    2. Inspect the 1-cc syringe (P/N 016388) for damage. If necessary, replace the syringe. Pump not primed properly Prime the pump (see “Priming the Pump (Standard Procedure)” page 90). Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 82: Abnormal Retention Time Or Selectivity

    Verify that the cell front cover is flush against the component panel. If necessary, tighten the two mounting screws. (This ensures an electrical connection between the cell and the connector inside the Dionex Aquion RFIC.) No flow from pump This condition has several possible causes; for more information, see “Pump Does Not Start”...

  • Page 83: Baseline Noise Or Drift

    40 °C (40 and 104 °F). 2. Make sure air conditioning and heating vents are directed away from the Dionex Aquion RFIC, and that the front door of the system is closed. Insufficient system Allow a longer system equilibration time (up to 2 hours) equilibration after changes before starting operation.

  • Page 84: Hardware Not Present

     Contact Technical Support for Dionex products for assistance. Note Dionex Aquion RFIC electronics components cannot be serviced by the user. Leak Sensor Wet This error occurs when liquid accumulates in the drip tray at the bottom of the Dionex...
  • Page 85 Contact Technical Support for Dionex products for assistance. IMPORTANT Never select the Always On option for routine operation. The Always On option should be selected only when testing the vacuum degas assembly. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 86 Troubleshooting Troubleshooting System Component Symptoms Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 87: Chapter 6 Service

    Service This chapter describes Dionex Aquion RFIC service and repair procedures that users may perform. All procedures not included here, including electronics-related repair procedures, must be performed by Thermo Fisher Scientific personnel. For assistance, contact Technical Support for Dionex products. In the U.S. and Canada, call 1-800-532-4752. Outside the U.S.

  • Page 88: Isolating A Restriction In The Liquid Lines

    The connection at which the pressure drops abnormally indicates the point of restriction. IMPORTANT The numbers on the flow schematic indicate the order in which liquid flows through the system components. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 89: Replacing Tubing And Fittings

    (see “Replacing Tubing and Fittings” page 73). Replacing Tubing and Fittings The Dionex Aquion RFIC is plumbed with the tubing and tubing assemblies listed below. Tubing size and type Color Used for... 0.125-mm (0.005-in) ID PEEK...

  • Page 90: Rebuilding The Injection Valve Or Auxiliary Valve

     1. Turn off the pump from the Chromeleon ePanel. 2. Open the Dionex Aquion RFIC front door. 3. Disconnect each liquid line connected to the valve. 4. Follow the instructions provided in the Injection Valve Rebuild Kit to replace the rotor seal and stator face.

  • Page 91: Replacing The Auxiliary Valve Pod

     1. Turn off the pump flow from the Chromeleon ePanel. 2. Open the Dionex Aquion RFIC front door. 3. Disconnect each liquid line connected to the auxiliary valve. 4. Unscrew the black locking ring on the front of the valve (see...

  • Page 92: Cleaning And Replacing The Pump Check Valves

     1. Turn off the pump from the Chromeleon ePanel. 2. Turn off the Dionex Aquion RFIC main power switch. 3. To prevent contamination of pump parts, put on standard disposable laboratory rubber gloves (powder-free, particle-free, and oil-free) before disassembling the pump head.
  • Page 93 7. Install the inlet check valve assembly (P/N 045722) on the bottom of the primary pump head. 8. Install the outlet check valve assembly (P/N 045721) on the top of the primary pump head. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 94: Replacing A Pump Piston Seal And Piston Rinse Seal

    IMPORTANT Overtightening may damage the pump head and check valve housing and crush the check valve seats. 10. Reconnect the liquid lines. 11. Close the front door. 12. Turn on the Dionex Aquion RFIC main power switch. 13. Prime the pump (see “Priming the Pump (Standard Procedure)” page 90).
  • Page 95 3. Disconnect all tubing connections to the pump head. 4. Locate the open-end wrench (P/N 014605) provided in the Dionex Aquion RFIC Ship Kit (P/N 064375). Use the wrench to remove the two acorn nuts from the pump head...
  • Page 96 Hold the seal guide with the flat side facing up. b. To dislodge the piston rinse seal, gently insert the shaft of the piston through the small hole in the center of the seal guide (see Figure 32). Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 97 3. Using a squirt bottle or a plastic syringe, fill the head cavity with deionized water through the piston opening (see Figure 33). Use ASTM filtered, Type I (18 megohm-cm) deionized water that meets the specifications listed in “Deionized Water Requirements for IC” page Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 98 Note If necessary, lubricate the seal with a small amount of 0.2 micron filtered isopropyl alcohol to facilitate insertion. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 99: Replacing A Pump Piston

    3. Reinstall the head and piston assembly. Use a wrench to tighten the nuts evenly (12 in-lb torque). To complete the procedure  1. Reconnect all liquid lines to the pump head. 2. Turn on the Dionex Aquion RFIC main power switch. 3. Prime the pump (see “Priming the Pump (Standard Procedure)” page 90).
  • Page 100 Note It is normal to encounter resistance after several rotations of the knob, as the O-ring is being pushed into the cavity of the pump head. 7. Turn on the Dionex Aquion RFIC main power switch. 8. Prime the pump (see “Priming the Pump (Standard Procedure)”...

  • Page 101: Replacing The Suppressor

    To replace the suppressor  1. Turn off the pump from the Chromeleon ePanel. 2. Open the Dionex Aquion RFIC front door. 3. Disconnect the two eluent lines and two regenerant lines from the suppressor. 4. Remove the suppressor from the system: a.

  • Page 102: Replacing The Eluent Valve

    Replacing the Eluent Valve To replace the eluent valve  1. Turn off the Dionex Aquion RFIC main power switch. 2. Open the Dionex Aquion RFIC front door. 3. To prevent an eluent leak during the valve replacement procedure: Disconnect the eluent line from the reservoir cap before proceeding.
  • Page 103 Figure 40. Eluent valve cable Disconnect cables here Eluent valve Eluent valve cable 8. Remove the mounting plate on the old valve by unscrewing and removing the two screws shown in Figure 41. Save the screws. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 104: Replacing The Leak Sensor

    10. Attach the mounting plate to the new valve, using the screws removed in Step 11. Connect the cable from the new valve to the Dionex Aquion RFIC cable. Feed the cables back inside the Dionex Aquion RFIC. 12. Hold the new valve with the ports facing up and align the valve with the component panel.
  • Page 105 6. Connect the cable from the new leak sensor (P/N 058053) to the connector on the other cable. 7. Feed the cables back inside the Dionex Aquion RFIC. 8. Align the leak sensor with the component panel opening and finger-tighten the screw on the front of the sensor.

  • Page 106: Priming The Pump (Standard Procedure)

    Figure 44. Priming the eluent lines Secondary Pump Head Waste Valve (close) Port Primary Pump Head Priming Valve 10 mL Syringe (open) (P/N 079803) 3. Open the priming valve by turning it one-quarter to one-half turn counterclockwise. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 107 OK. The pump will begin pumping at about 3 mL/min. 6. Monitor the pump waste line for air bubbles. When no air bubbles are visible in the waste line, close the waste valve. Do not overtighten the waste valve. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 108: Priming The Pump With Isopropyl Alcohol

    10. Under Pump_ECD, click the Prime button to flush the heads with eluent. IMPORTANT Isopropyl alcohol may damage some columns. Thoroughly rinse the alcohol from the pump as described in Step 9 Step Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 109: Replacing The Dionex Egc

    Figure 47) from the cartridge by unscrewing the VENT luer lock from the luer adapter. Tip If necessary, use a wrench to hold the luer adapter in place while unscrewing the vent line. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 110 7. Turn the Dionex EGC upside down and set it in the cartridge service area on top of the Dionex Aquion RFIC (see Figure 48 Figure 49). Figure 48. Dionex EGC service area Service area Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 111  1. Remove the new Dionex EGC from the shipping box. 2. Orient the Dionex EGC with the fittings facing up and remove the plugs from the INLET ports (see Figure 51). OUTLET Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 112 Figure 51. Dionex EGC inlet and outlet port lines OUTLET INLET 3. Set the Dionex EGC in the service area on top of the Dionex Aquion RFIC. Orient the cartridge with the electrical cable toward the Dionex EGC holder (see Figure 52).
  • Page 113 The lines are designed to coil neatly inside the holder. 9. Remove the plug from the connector on the Dionex Aquion RFIC top cover. 10. Orient the blue 4-pin electrical cable connector for the Dionex EGC as shown in Figure 54.
  • Page 114 The serial number is printed on the Dionex EGC label. 1. Open the Instrument Configuration Manager. 2. Double-click the Aquion IC System icon under the instrument. 3. In the Properties dialog box, click the Eluent Generator tab (see Figure 55). Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 115 OUTLET container. 3. Select these operating conditions on the Chromeleon ePanel: a. Set the pump flow rate to 1.00 mL/min. b. Set the Dionex EGC concentration to 50 mM. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 116: Replacing A Carbonate Cartridge

    2. Disconnect the Dionex EGC electrical cable from the connector on the Dionex Aquion RFIC top cover (see Figure 3. Disconnect the Dionex CR-TC electrical cable from the connector on the top CR-TC cover. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 117 7. Turn the Dionex EGC upside down and set it in the cartridge service area on top of the Dionex Aquion RFIC (see Figure 57 Figure 58). Figure 57. Dionex EGC service area Service area Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 118  1. Remove the new Dionex EGC from the shipping box. 2. Orient the Dionex EGC with the fittings facing up and remove the plugs from the INLET ports (see Figure 60). OUTLET Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 119 Figure 60. Dionex EGC inlet and outlet port lines OUTLET INLET 3. Set the Dionex EGC in the service area on top of the Dionex Aquion RFIC. Orient the cartridge with the electrical cable toward the Dionex EGC holder (see Figure 61).
  • Page 120 The lines are designed to coil neatly inside the holder. Figure 62. Dionex EGC installed in the holder 8. Remove the plug from the connector on the Dionex Aquion RFIC top cover. 9. Orient the blue 4-pin electrical cable connector for the Dionex EGC as shown in Figure 63.
  • Page 121 3. In the Properties dialog box, click the Eluent Generator tab (see Figure 64). Figure 64. Properties dialog box: Eluent Generator tab page 4. Select the EGC-1 check box. 5. Enter the serial number of the Dionex EGC. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 122 Carbonate Mixer Kit, 4 mm). 2. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) and a 10-32 to 10-32 coupler (P/N 042627) in the Dionex Aquion RFIC Ship Kit (P/N 064375). Dionex Aquion RFIC Operator’s Manual...
  • Page 123 Turn on the Dionex EGC current. 4. Run at the selected settings (1.00 mL/min at 9 mM) for 30 minutes. 5. Turn off the Dionex EGC current. 6. Turn off the pump. 7. Remove the backpressure coil. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...

  • Page 124: Replacing The Dionex Cr-Tc

    6. Grasp the top of the Dionex CR-TC and lift up to disconnect it from the holder. 7. Disconnect the liquid lines from the four ports on the Dionex CR-TC (see Figure 67). Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 125 Dionex EGC holder. Continue pushing down until the Dionex CR-TC snaps into place (see Figure 68). Figure 68. Dionex CR-TC installed in Dionex EGC holder Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 126 ASTM filtered, Type 1 (18 megohm-cm) deionized water is pumped through the Dionex CR-TC for 10 minutes (bypassing the guard column, separator column, and suppressor). Figure 70 illustrates the liquid flow path through the Dionex CR-TC during the hydration procedure. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 127 Service Replacing the Dionex CR-TC Figure 70. Dionex CR-TC plumbing for hydration 1. Locate the 10-32 to 1/4-28 coupler (P/N 042806) provided in the Dionex Aquion RFIC Ship Kit (P/N 064375). 2. Use the coupler to connect the line labeled...

  • Page 128: Replacing The Dionex Egc 500 Carbonate Mixer

    During initial system installation, the carbonate mixer is attached directly to the inside of the front door (with double-sided tape) or mounted in two 3/4-in U style mounting clips. For details, refer to the Dionex Aquion RFIC installation instructions. To remove the old carbonate mixer ...
  • Page 129 Figure 72. Disconnecting the Dionex EGC inlet and pump outlet TO PUMP/DAMPER (Disconnect this line) Pump outlet line (green tubing) 3. Disconnect the line from the TO EGC CO3 MIXER IN TO INJ VALVE IN - P/ELUENT OUT line. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 130 • The carbonate mixer for 4-mm columns has a void volume of about 16 mL. At a flow rate of 1.0 mL/min, it takes about 16 minutes to fill the mixer. 11. Turn off the pump. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 131 Assembly 1. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) and a 10-32 to 10-32 coupler (P/N 042627) provided in the Dionex Aquion RFIC Ship Kit (P/N 064375). 2. Use the coupler to connect the...

  • Page 132: Replacing The Dionex Egc Holder And Degas Assembly

    Replacing the Dionex EGC Holder and Degas Assembly To remove the Dionex EGC from the system  1. Turn off the Dionex Aquion RFIC main power switch. 2. Disconnect the following Dionex EGC holder liquid lines: • The line labeled...
  • Page 133 6. Lift the cartridge straight up and out of the Dionex EGC holder. 7. Turn the cartridge upside down and set it in the cartridge service area on top of the Dionex Aquion RFIC (see Figure 76). Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 134 2. Grasp the top of the Dionex CR-TC and lift up to disconnect it from the holder. 3. Disconnect the liquid lines from the four ports on the Dionex CR-TC (see Figure 78). Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 135 6. After checking that none of the lines are caught under the Dionex CR-TC, begin pushing the column onto the metal stud inside the Dionex EGC holder. Continue pushing down until the Dionex CR-TC snaps into place (see Figure 80). Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 136 Figure 81. Dionex CR-TC electrical cable CR-TC electrical cable 8. Turn the Dionex EGC holder right-side up and set it on top of the Dionex Aquion RFIC. 9. Direct the line from the top of the Dionex Aquion RFIC to WASTE, GAS SEPARATOR the rear panel.
  • Page 137 1. Remove the new Dionex EGC holder (P/N 058069) from the shipping box. 1. Set the Dionex EGC holder in place on top of the Dionex Aquion RFIC. 2. Feed the following liquid lines through the tubing chase to the Dionex Aquion RFIC component panel: •...

  • Page 138: Changing The Main Power Fuses

    1. Turn off the Dionex Aquion RFIC main power switch. DANGER HIGH VOLTAGE—Disconnect the main power cord from its source and also from the rear panel of the Dionex Aquion RFIC. DANGER HAUTE TENSION—Débranchez le cordon d'alimentation principal de sa source et du panneau arrière du Dionex Aquion RFIC.
  • Page 139 Service Changing the Main Power Fuses 2. The fuse holder is part of the main power receptacle on the Dionex Aquion RFIC rear panel (see Figure 83). To remove the fuse holder, squeeze the tab on the top of the holder to release it and pull the holder straight out of its compartment.
  • Page 140 Service Changing the Main Power Fuses Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 141: Appendix A Specifications

    Upper limit of 0 to 35 MPa (0 to 5000 psi) in increments of one unit (MPa or psi); lower limit can be set to up to one unit (MPa or psi) lower than the upper limit Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 142 ±0.1 nS/cm when background conductivity is 0 to 150 μS/cm ±2 nS/cm when background conductivity is 151 to 3200 μS/cm Filter Rise times from 0 to 10 s; user-selectable Temperature compensation Fixed at 1.7% per 1 °C at cell temperature Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 143 Electrolytic suppression 2 mm and 4 mm anion and cation, membrane suppression bed types available Electrolytic suppression with 2 mm and 4 mm anion and cation, membrane External Water Mode suppression bed types available Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 144 Dionex ADRS 600 (4 mm): <50 μL Dionex AERS 500 Carbonate (2 mm): <15 μL Dionex AERS 500 Carbonate (4 mm): <50 μL Cations: Dionex CDRS 600 (2 mm): <15 μL Dionex CDRS 600 (4 mm): <50 μL Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 145 56.1 × 22.4 × 53.3 cm (22.1 × 8.8 × 21.0 in) Note: Dimensions do not include Dionex EGC or eluent reservoir. Weight 51 lbs (23 kg) net weight 64 lbs (29 kg) with boxes Decibel level <52 dBA Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 146 Specifications Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 147: Appendix B Ttl And Relay Control

    TTL and Relay Connections • Controlling TTL and Relay Outputs TTL and Relay Connections The 12-pin connector strip on the Dionex Aquion RFIC rear panel provides two relay outputs, two TTL outputs, and four TTL inputs (see Figure 84). Figure 84. TTL and relay connector on rear panel...
  • Page 148 IMPORTANT When attaching wires to the connector plug, do not allow stray strands of wire to short to the adjoining position on the connector. b. Plug the connector into the 12-pin connector on the Dionex Aquion RFIC rear panel. Dionex Aquion RFIC Operator’s Manual...
  • Page 149 TTL and Relay Control TTL and Relay Connections c. Connect the wires from the Dionex Aquion RFIC connector plug to the TTL or relay connector pins on the other module(s). Additional connector plugs are provided with other Dionex modules. Note Verify the polarity of each connection. Connect signal wires to signal (+) pins and ground wires to ground (-) pins.
  • Page 150 Normal Edge, is compatible with the output signals provided by the Dionex modules. If the device connected to the Dionex Aquion RFIC does not send a normal edge signal, select the appropriate control type. To select the correct control type, refer to both the documentation provided with the controlling device and the information below.

  • Page 151: Controlling Ttl And Relay Outputs

    Controlling TTL and Relay Outputs Controlling TTL and Relay Outputs The Dionex Aquion RFIC provides two TTL outputs and two relay contacts for control of functions in external devices, such as an integrator or autosampler. The relay outputs can be used to switch any low-voltage control.
  • Page 152 TTL and Relay Control Controlling TTL and Relay Outputs Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 153: Appendix C Reordering

    Dionex EGC III KOH Potassium Hydroxide Eluent Generator Cartridge 074534 Dionex EGC III LiOH Lithium Hydroxide Eluent Generator Cartridge 074533 Dionex EGC III NaOH Sodium Hydroxide Eluent Generator Cartridge 088453 Dionex EGC 500 K Potassium Carbonate Eluent Generator Cartridge Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 154 Dionex CDRS 600 Cation Dynamically Regenerated Suppressor (4 mm) 085028 Dionex AERS 500 Carbonate Electrolytically Regenerated Suppressor (2 mm) 085029 Dionex AERS 500 Carbonate Electrolytically Regenerated Suppressor (4 mm) 045460 Suppressor waste, gas separator tube Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 155 960779 USB cable, 5 m (16 ft) 060392 External USB hub Preventive Maintenance Kits 057954 Dionex Aquion RFIC Preventive Maintenance Kit 075000 Dionex AS-AP Preventive Maintenance Kit 60-065335 Dionex AS-DV Preventive Maintenance Kit Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 156 Reordering Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 157: Appendix D Faq

    For details, refer to the Chromeleon Help or user’s guide. When should I remake standards? Standards are used only for calibration and should always be made fresh (they have a lifetime of only one week). Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 158 When should I replace the eluent generator cartridge? The Dionex Aquion RFIC monitors Dionex EGC use. When it is time to replace the cartridge, a message is displayed in the Chromeleon audit trail. To view the remaining cartridge life, click EG Settings on the Chromeleon ePanel.
  • Page 159 How do I configure and operate the auxiliary valve? The auxiliary valve must be installed on-site by Thermo Fisher Scientific field service personnel. For details about valve operation, see “Auxiliary Valve (Optional)” page Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 160 Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 161: Appendix E Glossary

    Capacity factor is a dimensionless measure of retention which is independent of column length or eluent flow rate. It is calculated as follows: – -------------- k′ Where: t = retention time = retention time of unretained solute (column void volume) Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 162 Describes the relative separation of the band maxima between two adjacent peaks. Selectivity can be determined by the following: a = (t )/(t Where: t and t = retention time of components 1 and 2, respectively = retention time of unretained components (void volume) Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 163 Where: L = the column length (in mm) N = the number of theoretical plates Ion-Exchange Capacity The number of active ion exchange sites in a given weight or volume of resin; this is usually expressed in meq/g or meq/mL. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 164 R is proportional to the square root of efficiency (N). A value of R = 1.5 represents “baseline separation” of the two peaks. Retention Time The time from injection to peak maximum; the basis for identification of a species in chromatographic analysis. Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 165 The volume occupied by the eluent in a packed column. This volume includes the volume between the injection valve and the column and between the column and the conductivity cell. Unretained components are eluted in the void volume. Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 166 Glossary Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

  • Page 167: Index

    See also AS-AP Autosampler See also AS-DV Autosampler Autosampler specifications Automation Calibration curve Online filtration Definition Online sample degassing Capacity factor Sequential injections Definition Simultaneous injections Carbonate eluent Autozero Calibration standards Via Chromeleon Water dip Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 168 Column selectivity Degas tubing assembly (EGC) Definition Degassing eluents Columns Deionized water specifications Bed supports clogged Detection description Cleaning Detector specifications Contamination Electronics Damage from IPA Linearity Storing Range Command window (Chromeleon) Resolution Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 169 Suppressor stopped for zero flow rate Electromagnetic compatibility Eluent Aqueous Filter, end-line Delivery modes Bacterial contamination Filtering Dirty or clogged Monitoring the supply Installation Selecting the degas mode Fittings Eluent bottle Leaking See Reservoir, eluent Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 170 Manual sample processing Inverted edge TTL input control MSA eluent generation Inverted pulse TTL input control Flow schematic 13–15 Ion chromatography overview Ion exchange Ion-exchange capacity NaOH eluent generation Definition Flow schematic 13–15 Isopropyl alcohol Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 171 Power Main power cord Queue (Chromeleon) Receptacle Requirements Turning on Rear panel Power-up conditions Analog output connector Pressure limits Power receptacle High pressure limit triggered TTL and relay connector Low pressure limit triggered Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 172 25–26 Eluent generation Changing Front panel Injecting Injection valve Injection valve connections Suppressor Loading Vacuum degas assembly Reordering Standards Sample processing 43–44 When to remake Automated (batch) Sulfite in samples Manual Suppression description Dionex Aquion RFIC Operator’s Manual Thermo Scientific...
  • Page 173 Definition Valve Top cover See Auxiliary valve Electrical connectors See Eluent valve Eluent generator cartridge See Injection valve Eluent reservoir See Waste valve Tubing chase Void volume Troubleshooting Definition Baseline noise or drift Thermo Scientific Dionex Aquion RFIC Operator’s Manual...
  • Page 174 Index: W Warranty Waste container 116, 120–121 Waste lines Waste, gas separator tube Waste valve Opening O-ring replacement procedure Waste, gas separator tube Water dip Water samples WEEE compliance Weekly maintenance Weight Dionex Aquion RFIC Operator’s Manual Thermo Scientific...

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